Electron microscopy

Publisher Summary This chapter provides a detailed methodology of some of the basic electron microscopic (EM) techniques currently used in virology. The chapter is subdivided into four sections: a general introduction, negative staining procedures, thin sectioning procedures, and briefly scanning EM. Following a brief discussion of the principles, each technique is given in the chapter in sufficient detail to be followed as a recipe. Modifications are included within the methodology. The size, equipment, and organization of an EM unit naturally vary according to the service requirements. There are some basic requirements that need to be considered. Immune electron microscopy is the visualization of the reaction between an antigen, usually a virus, and an antibody and is used to detect either component of this reaction. Thus, the technique has been used to detect serological responses to such viruses as Norwalk virus. The technique has assisted in the identification of rubella virus and been diagnostically used to identify Hepatitis B virus infection and viruses associated with diarrhea.

[1]  M. Boussoukaya,et al.  High quantum yield from photofield emitters , 1989 .

[2]  V. P. Spiridonov,et al.  A stroboscopical gas-electron diffraction method for the investigation of short-lived molecular species , 1983 .

[3]  W. Tornow,et al.  Time Resolved Tem of Laser-Induced Phase Transitions in a-Ce And a-Si/Al-Films , 1986 .

[4]  M. Dizdaroglu.  Measurement of radiation-induced damage to DNA at the molecular level. , 1992, International journal of radiation biology.

[5]  O. Bostanjoglo,et al.  Tracing fast phase transitions by electron microscopy , 1980 .

[6]  Alexander W. Chao,et al.  Physics Of Collective Beam Instabilities In High Energy Accelerators , 1993 .

[7]  Richard Henderson,et al.  Cryo-protection of protein crystals against radiation damage in electron and X-ray diffraction , 1990, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[8]  R. Becker,et al.  Low-Energy Electron Diffraction during Pulsed Laser Annealing: A Time- Resolved Surface Structural Study , 1984 .

[9]  P. Michelato.  Photocathodes for RF photoinjectors , 1997 .

[10]  O. Bostanjoglo,et al.  High-speed electron microscopy of nanocrystallization in AlNi films by nanosecond laser pulses† , 1995 .

[11]  Martin Reiser,et al.  Theory and Design of Charged Particle Beams , 1994 .

[12]  Ahmed H. Zewail,et al.  Ultrafast Electron Diffraction. 5. Experimental Time Resolution and Applications , 1994 .

[13]  H. Michaelson.  The work function of the elements and its periodicity , 1977 .

[14]  W. Tornow,et al.  Nanosecond transmission electron microscopy and diffraction , 1987 .

[15]  O. Bostanjoglo,et al.  PULSED PHOTOELECTRON MICROSCOPE FOR IMAGING LASER-INDUCED NANOSECOND PROCESSES , 1997 .

[16]  J. Harper,et al.  Photoemission from thin-film lanthanum hexaboride , 1990 .

[17]  J. W. Herman,et al.  Picosecond time‐resolved surface‐lattice temperature probe , 1990 .

[18]  O. Bostanjoglo,et al.  Producing high-current nanosecond electron pulses with a standard tungsten hairpin gun , 1987 .

[19]  O. Bostanjoglo,et al.  Impulse stimulated crystallization of Sb films investigated by time resolved TEM , 1981 .

[20]  J. L. Adamski,et al.  Results of the Boeing pulse compression and energy recovery experiments , 1997 .

[21]  A. R. Faruqi,et al.  Cooled CCD camera with tapered fibre optics for electron microscopy , 1997 .

[22]  Anthony P. Rood,et al.  Combined flash-photolysis and gas-phase electron-diffraction studies of small molecules , 1984 .

[23]  R. Tornow,et al.  Nanosecond double-frame electron microscopy of fast phase transitions , 1989 .

[24]  O. Bostanjoglo,et al.  Liquid motion in laser pulsed Al, Co and Au films , 1997 .

[25]  Wiedemann,et al.  Generation and measurement of 50-fs(rms) electron pulses. , 1994, Physical review letters.

[26]  O. Bostanjoglo,et al.  Ultrasonically Induced Magnetic Reversals Observed by Stroboscopic Electron Microscopy , 1977 .

[27]  O. Bostanjoglo,et al.  High speed transmission electron microscopy of laser quenching , 1993 .

[28]  Herman,et al.  Superheating of Pb(111). , 1992, Physical review letters.

[29]  O. Bostanjoglo,et al.  Pulsed TEM: a new method to detect transient structures in fast phase transitions , 1983 .

[30]  G. Hoffmann,et al.  Time-Resolved TEM of Transient Effects in Pulse Annealing of Ge and Ge–Te Films , 1982 .

[31]  Lothar Schäfer,et al.  Real‐time data acquisition for gas electron diffraction , 1984 .

[32]  G. H. Jansen.  Coulomb Interactions in Particle Beams , 1988 .

[33]  Ciaran Lewis,et al.  Picosecond X-Ray Chronoscopy , 1975, Other Conferences.

[34]  C. Travier.  An introduction to photo-injector design , 1994 .

[35]  B. Schäfer,et al.  Laser driven thermionic electron gun , 1992 .

[36]  J. Fryer.  The effect of dose rate on imaging aromatic organic crystals , 1987 .

[37]  O. Bostanjoglo,et al.  Resonance oscillations of magnetic domain walls and bloch lines observed by stroboscopic electron microscopy , 1980 .

[38]  O. Bostanjoglo.  Time-Resolved TEM of Pulsed Crystallization of Amorphous Si and Ge Films , 1982, April 16.

[39]  D. Knight,et al.  Staining methods for sectioned material , 1977 .

[40]  Lothar Schäfer,et al.  Time-resolved gas electron diffraction study of the 193-nm photolysis of 1,2-dichloroethenes , 1993 .

[41]  O. Bostanjoglo,et al.  Ablation of metal films by picosecond laser pulses imaged with high‐speed electron microscopy , 1994 .

[42]  J. Spence,et al.  Brightness measurements of nanometer-sized field-emission-electron sources , 1993 .

[43]  John J. Bozzola,et al.  Electron microscopy : principles and techniques for biologists , 1992 .

[44]  G. Mourou,et al.  Time-Resolved Laser-Induced Phase Transformation in Aluminum , 1984, Topical Meeting on Ultrafast Phenomena.

[45]  D. Neuffer.  Longitudinal Motion in High Current Ion Beams - A Self-Consistent Phase Space Distribution with an Envelope Equation , 1979, IEEE Transactions on Nuclear Science.

[46]  J. Derbyshire.  ELECTRON MICROSCOPY IN DIAGNOSTIC VIROLOGY. A PRACTICAL GUIDE AND ATLAS , 1988 .

[47]  H. G. Hereward,et al.  Effects of Space Charge and Reactive Wall Impedance on Bunched Beams , 1975, IEEE Transactions on Nuclear Science.

[48]  R. Egerton.  Electron Energy-Loss Spectroscopy in the Electron Microscope , 1995, Springer US.

[49]  Walter F. Kosonocky,et al.  360/spl times/360 element three-phase very high frame rate burst image sensor: design, operation and performance , 1997 .

[50]  D. Edwards,et al.  An Introduction to the Physics of High Energy Accelerators , 1992 .

[51]  F. G. Zaki.  Principles and Techniques of Electron Microscopy , 1975 .

[52]  O. Bostanjoglo,et al.  Kinetics of Laser‐Induced Crystallization of Amorphous Germanium Films , 1985 .

[53]  O. Bostanjoglo,et al.  Hydrodynamic instabilities in laser pulse‐produced melts of metal films , 1996 .

[54]  M. Uesaka,et al.  Generation of high-current (1kA) subpicosecond electron single pulse , 1997 .

[55]  L. Serafini.  Micro-bunch production with radio frequency photoinjectors , 1996 .

[56]  Enhancement of photoelectric emission sensitivity of tungsten by potassium ion implantation , 1995 .

[57]  C. Joshi,et al.  Photoemission from diamond and fullerene films for advanced accelerator applications , 1996 .

[58]  Picosecond electron diffraction , 1982 .

[59]  Ahmed H. Zewail,et al.  Clocking transient chemical changes by ultrafast electron diffraction , 1997, Nature.

[60]  Glyn Colebrooke.  Virus morphology , 1989, Journal of Hospital Infection.

[61]  W. Tornow,et al.  Nanosecond-exposure electron microscopy of laser-induced phase transformations , 1987 .