Structured Illumination Microscopy

Structured illumination microscopy (SIM) is a fluorescence light microscopy method that relies on modulation of the excitation light by spatial patterning to break the diffraction barrier. SIM provides a resolution gain of only a factor of two, yet has the least stringent requirement for fluorophores selection and readily accommodates multiple color labeling. These properties make SIM the method of choice in super-resolution microscopy when imaging several structures simultaneously and with high-throughput, due to its flexibility and relative ease of sample optimization. In this article, we provide an introduction to the principles of SIM, detail its impact in cell biology, and present the most recent developments of this imaging technology.

[1]  Ulrike Engel,et al.  Structured illumination superresolution imaging of the cytoskeleton. , 2014, Methods in cell biology.

[2]  Ilan Davis,et al.  A cell biologist's guide to high resolution imaging. , 2012, Methods in enzymology.

[3]  Thomas Cremer,et al.  The potential of 3D‐FISH and super‐resolution structured illumination microscopy for studies of 3D nuclear architecture , 2012, BioEssays : news and reviews in molecular, cellular and developmental biology.

[4]  Andrew G. York,et al.  Instant super-resolution imaging in live cells and embryos via analog image processing , 2013, Nature Methods.

[5]  Hari Shroff,et al.  Faster fluorescence microscopy: advances in high speed biological imaging. , 2014, Current opinion in chemical biology.

[6]  Jiri Bartek,et al.  Dynamics and Organization of Cortical Microtubules as Revealed by Superresolution Structured Illumination Microscopy1[W] , 2014, Plant Physiology.

[7]  Kerry Bloom,et al.  Bending the Rules: Widefield Microscopy and the Abbe Limit of Resolution , 2014, Journal of cellular physiology.

[8]  L. V. van Vliet,et al.  Three-dimensional structured illumination microscopy using Lukosz bound apodization reduces pixel negativity at no resolution cost. , 2014, Optics express.

[9]  M. Davidson,et al.  Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination , 2012, Proceedings of the National Academy of Sciences.

[10]  M. Gustafsson,et al.  Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. , 2008, Biophysical journal.

[11]  Natalie Elia,et al.  Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission , 2011, Proceedings of the National Academy of Sciences.

[12]  Lothar Schermelleh,et al.  RecA bundles mediate homology pairing between distant sisters during DNA break repair , 2013, Nature.

[13]  K. Ramamurthi,et al.  Cellular Architecture Mediates DivIVA Ultrastructure and Regulates Min Activity in Bacillus subtilis , 2011, mBio.

[14]  M. Gustafsson,et al.  Super-resolution 3D microscopy of live whole cells using structured illumination , 2011, Nature Methods.

[15]  S. Hell.  Far-Field Optical Nanoscopy , 2007, Science.

[16]  B. Alberts.  The Cell as a Collection of Protein Machines: Preparing the Next Generation of Molecular Biologists , 1998, Cell.

[17]  Jason R Swedlow,et al.  Quantitative fluorescence microscopy and image deconvolution. , 2007, Methods in cell biology.

[18]  Bryant B. Chhun,et al.  Super-Resolution Video Microscopy of Live Cells by Structured Illumination , 2009, Nature Methods.

[19]  Thomas Müller-Reichert,et al.  Cortical Constriction During Abscission Involves Helices of ESCRT-III–Dependent Filaments , 2011, Science.

[20]  Xiumin Yan,et al.  The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis , 2013, Nature Cell Biology.

[21]  J. Swedlow,et al.  A workingperson's guide to deconvolution in light microscopy. , 2001, BioTechniques.

[22]  G. C. Rogers,et al.  Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization , 2012, Nature Cell Biology.

[23]  J. Lippincott-Schwartz,et al.  Incisive Imaging and Computation for Cellular Mysteries: Lessons from Abscission , 2013, Cell.

[24]  Hari Shroff,et al.  Resolution Doubling in Live, Multicellular Organisms via Multifocal Structured Illumination Microscopy , 2012, Nature Methods.

[25]  E. Betzig,et al.  Nonmuscle Myosin II Isoforms Coassemble in Living Cells , 2014, Current Biology.

[26]  Rainer Heintzmann,et al.  Breaking the resolution limit in light microscopy. , 2006, Briefings in functional genomics & proteomics.

[27]  I. Young,et al.  Image filtering in structured illumination microscopy using the Lukosz bound. , 2013, Optics express.

[28]  Leigh G. Monahan,et al.  3D-SIM Super Resolution Microscopy Reveals a Bead-Like Arrangement for FtsZ and the Division Machinery: Implications for Triggering Cytokinesis , 2012, PLoS biology.

[29]  Nathalie Spassky,et al.  Centriole amplification by mother and daughter centrioles differs in multiciliated cells , 2014, Nature.

[30]  R. Heintzmann,et al.  Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport? , 2013, Biophysical journal.

[31]  E. Nigg,et al.  Human Cep192 and Cep152 cooperate in Plk4 recruitment and centriole duplication , 2013, Journal of Cell Science.

[32]  M. Neil,et al.  Remodelling of Cortical Actin Where Lytic Granules Dock at Natural Killer Cell Immune Synapses Revealed by Super-Resolution Microscopy , 2011, PLoS biology.

[33]  Jerome Mertz,et al.  Optical sectioning microscopy with planar or structured illumination , 2011, Nature Methods.

[34]  J. Löwe,et al.  FtsA forms actin‐like protofilaments , 2012, The EMBO journal.

[35]  S. Hell,et al.  Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[36]  Jean-Michel Morel,et al.  A Review of Image Denoising Algorithms, with a New One , 2005, Multiscale Model. Simul..

[37]  H. Ewers,et al.  A simple, versatile method for GFP-based super-resolution microscopy via nanobodies , 2012, Nature Methods.

[38]  Laurence Pelletier,et al.  Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material , 2012, Nature Cell Biology.

[39]  M. Gustafsson,et al.  Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy , 2008, Science.

[40]  Liang Gao,et al.  3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy , 2014, Nature Protocols.

[41]  J. J. Macklin,et al.  Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution , 2011, Proceedings of the National Academy of Sciences.

[42]  L. Schermelleh,et al.  Functional nuclear organization of transcription and DNA replication: a topographical marriage between chromatin domains and the interchromatin compartment. , 2010, Cold Spring Harbor symposia on quantitative biology.

[43]  R. Heintzmann,et al.  Saturated patterned excitation microscopy--a concept for optical resolution improvement. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[44]  Eric A. Vitriol,et al.  Instantaneous inactivation of cofilin reveals its function of F-actin disassembly in lamellipodia , 2013, Molecular biology of the cell.

[45]  Bo Huang,et al.  Super-resolution optical microscopy: multiple choices. , 2010, Current opinion in chemical biology.

[46]  S. Hell.  Microscopy and its focal switch , 2008, Nature Methods.

[47]  Reto Fiolka,et al.  Seeing more with structured illumination microscopy. , 2014, Methods in cell biology.

[48]  P. Carlton,et al.  Three-dimensional structured illumination microscopy and its application to chromosome structure , 2008, Chromosome Research.

[49]  D. Agard,et al.  Amorphous no more: subdiffraction view of the pericentriolar material architecture. , 2014, Trends in cell biology.

[50]  M. Gustafsson.  Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy , 2000, Journal of microscopy.

[51]  Bernhard Goetze,et al.  Structure brings clarity: Structured illumination microscopy in cell biology , 2009, Biotechnology journal.

[52]  John W Sedat,et al.  OMX: a new platform for multimodal, multichannel wide-field imaging. , 2011, Cold Spring Harbor protocols.

[53]  D. Glover,et al.  Structured illumination of the interface between centriole and peri-centriolar material , 2012, Open Biology.

[54]  Mark Bates,et al.  Super-resolution fluorescence microscopy. , 2009, Annual review of biochemistry.

[55]  M. Davidson,et al.  Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens , 2012, Cell.

[56]  Carla Coltharp,et al.  Superresolution microscopy for microbiology , 2012, Cellular microbiology.

[57]  H. Leonhardt,et al.  A guide to super-resolution fluorescence microscopy , 2010, The Journal of cell biology.

[58]  U. Birk,et al.  Measurement of replication structures at the nanometer scale using super-resolution light microscopy , 2009, Nucleic acids research.

[59]  J. Yates,et al.  Cell-cycle regulation of formin-mediated actin cable assembly , 2013, Proceedings of the National Academy of Sciences.