Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering.

Electrospun fiber matrices composed of scaffolds of varying fiber diameters were investigated for potential application of severe skin loss. Few systematic studies have been performed to examine the effect of varying fiber diameter electrospun fiber matrices for skin regeneration. The present study reports the fabrication of poly[lactic acid-co-glycolic acid] (PLAGA) matrices with fiber diameters of 150-225, 200-300, 250-467, 500-900, 600-1,200, 2,500-3,000 and 3,250-6,000 nm via electrospinning. All fiber matrices found to have a tensile modulus from 39.23+/-8.15 to 79.21+/-13.71 MPa which falls in the range for normal human skin. Further, the porous fiber matrices have porosity between 38 to 60% and average pore diameters between 10 to 14 microm. We evaluated the efficacy of these biodegradable fiber matrices as skin substitutes by seeding them with human skin fibroblasts (hSF). Human skin fibroblasts acquired a well spread morphology and showed significant progressive growth on fiber matrices in the 350-1,100 nm diameter range. Collagen type III gene expression was significantly up-regulated in hSF seeded on matrices with fiber diameters in the range of 350-1,100 nm. Based on the need, the proposed fiber skin substitutes can be successfully fabricated and optimized for skin fibroblast attachment and growth.

[1]  Matthew J Dalby,et al.  Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands. , 2002, Experimental cell research.

[2]  Thomas Zimmerman,et al.  Prevention of Postsurgery-Induced Abdominal Adhesions by Electrospun Bioabsorbable Nanofibrous Poly(lactide-co-glycolide)-Based Membranes , 2004, Annals of surgery.

[3]  D. Brunette,et al.  Effects of substratum surface topography on the organization of cells and collagen fibers in collagen gel cultures. , 2002, Journal of biomedical materials research.

[4]  A F von Recum,et al.  Texturing of polymer surfaces at the cellular level. , 1991, Biomaterials.

[5]  P. Bongrand,et al.  Is there a predictable relationship between surface physical-chemical properties and cell behaviour at the interface? , 2004, European cells & materials.

[6]  S. Ramakrishna,et al.  Collagen-blended biodegradable polymer nanofibers: potential substrates for wound healing in skin tissue engineering , 2007 .

[7]  C. Lim,et al.  Recent development of polymer nanofibers for biomedical and biotechnological applications , 2005, Journal of materials science. Materials in medicine.

[8]  T J Sims,et al.  Presence of type III collagen in guinea-pig dermal scar. , 1976, The Biochemical journal.

[9]  D. Katti,et al.  Synthetic Biomedical Polymers for Tissue Engineering and Drug Delivery , 2003 .

[10]  Cato T Laurencin,et al.  Polymeric nanofibers as novel carriers for the delivery of therapeutic molecules. , 2006, Journal of nanoscience and nanotechnology.

[11]  I. K. Cohen,et al.  Quantitation of Collagen Types I and III during Wound Healing in Rat Skin , 1979, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[12]  Myung-Seob Khil,et al.  Electrospun nanofibrous polyurethane membrane as wound dressing. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[13]  G. Palmese,et al.  Synthesis of polymer–polymer nanocomposites using radiation grafting techniques , 2005 .

[14]  K. Rodgers,et al.  Comparative efficacy of nonsteroidal anti-inflammatory drugs and anti-thromboxane agents in a rabbit adhesion-prevention model. , 1995, Journal of investigative surgery : the official journal of the Academy of Surgical Research.

[15]  Burak Erman,et al.  Electrospinning of polyurethane fibers , 2002 .

[16]  D. Menzies.  Peritoneal adhesions. Incidence, cause, and prevention. , 1992, Surgery annual.

[17]  Shanta Raj Bhattarai,et al.  Hydrophilic nanofibrous structure of polylactide; fabrication and cell affinity. , 2006, Journal of biomedical materials research. Part A.

[18]  D. Brunette,et al.  Effects of titanium substratum and grooved surface topography on metalloproteinase-2 expression in human fibroblasts. , 1998, Journal of biomedical materials research.

[19]  C. Laurencin,et al.  A preliminary report on a novel electrospray technique for nanoparticle based biomedical implants coating: precision electrospraying. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[20]  C. Laurencin,et al.  Development of novel tissue engineering scaffolds via electrospinning , 2004, Expert opinion on biological therapy.

[21]  C. Compton,et al.  Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting. A light, electron microscopic and immunohistochemical study. , 1989, Laboratory investigation; a journal of technical methods and pathology.

[22]  Chi-Hwa Wang,et al.  Electrospun Micro- and Nanofibers for Sustained Delivery of Paclitaxel to Treat C6 Glioma in Vitro , 2006, Pharmaceutical Research.

[23]  P. Cristoforoni,et al.  Endogenous versus exogenous IL-10 in postoperative intraperitoneal adhesion formation in a murine model. , 1997, The Journal of surgical research.

[24]  Thomas J Webster,et al.  Mechanism(s) of increased vascular cell adhesion on nanostructured poly(lactic-co-glycolic acid) films. , 2005, Journal of biomedical materials research. Part A.

[25]  S. Ramakrishna,et al.  Applications of polymer nanofibers in biomedicine and biotechnology , 2005, Applied biochemistry and biotechnology.

[26]  Sheila MacNeil,et al.  Progress and opportunities for tissue-engineered skin , 2007, Nature.

[27]  H B Lee,et al.  Interaction of fibroblast cells on poly(lactide-co-glycolide) surface with wettability chemogradient. , 1999, Bio-medical materials and engineering.

[28]  L. Forrest.  Current concepts in soft connective tissue wound healing , 1983, The British journal of surgery.

[29]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[30]  D. Armstrong,et al.  The potential benefits of advanced therapeutic modalities in the treatment of diabetic foot wounds. , 2000, Journal of the American Podiatric Medical Association.

[31]  Christopher J Murphy,et al.  Biological length scale topography enhances cell-substratum adhesion of human corneal epithelial cells , 2004, Journal of Cell Science.

[32]  S. Golder,et al.  The effectiveness and cost-effectiveness of prophylactic removal of wisdom teeth. , 2000, Health technology assessment.

[33]  P. Baumgarten,et al.  Electrostatic spinning of acrylic microfibers , 1971 .

[34]  Cato T Laurencin,et al.  Electrospun nanofibrous structure: a novel scaffold for tissue engineering. , 2002, Journal of biomedical materials research.

[35]  T. Sheldon,et al.  Systematic reviews of wound care management: (3) antimicrobial agents for chronic wounds; (4) diabetic foot ulceration. , 2001, Health technology assessment.

[36]  K. Harding,et al.  Clinical review Science , medicine , and the future Healing chronic wounds , 2005 .

[37]  A. Goldstein,et al.  Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates. , 2006, Biomaterials.

[38]  S. Levenson,et al.  The Healing of Rat Skin Wounds , 1965, Annals of surgery.

[39]  A. Salgado,et al.  Nano- and micro-fiber combined scaffolds: A new architecture for bone tissue engineering , 2005, Journal of materials science. Materials in medicine.

[40]  W. Stolz,et al.  Localization of collagen alpha 1(I) gene expression during wound healing by in situ hybridization. , 1989, The Journal of investigative dermatology.