Cell Recordings with a CMOS High-density Microelectrode Array

Recordings have been performed with a CMOS-based microelectrode array (MEA) featuring 11'016 metal electrodes and 126 channels, each of which comprises recording and stimulation electronics for extracellular, bidirectional communication with electrogenic cells. The important features of the device include (i) high spatial resolution at (sub)cellular level with 3'200 electrodes per mm2 (diameter 7 mum, pitch 18 mum), (ii) a reconfigurable routing of the electrodes to the 126 channels, and (iii) low noise levels. Recordings from neonatal rat cardiomyocytes forming confluent layers and microtissues are shown. Moreover, signals from dissociated rat hippocampal neurons and from neurons in an acute cerebellar slice preparation are presented.

[1]  A. Aertsen,et al.  Two-dimensional monitoring of spiking networks in acute brain slices , 2001, Experimental Brain Research.

[2]  Steve M. Potter,et al.  An extremely rich repertoire of bursting patterns during the development of cortical cultures , 2006, BMC Neuroscience.

[3]  K. Wise,et al.  An implantable multielectrode array with on-chip signal processing , 1986 .

[4]  G. Gross,et al.  The use of neuronal networks on multielectrode arrays as biosensors. , 1995, Biosensors & bioelectronics.

[5]  B. Eversmann,et al.  A 128 × 128 CMOS bio-sensor array for extracellular recording of neural activity , 2003 .

[6]  Martin Fussenegger,et al.  Design of artificial myocardial microtissues. , 2004, Tissue engineering.

[7]  W. Rutten.  Selective electrical interfaces with the nervous system. , 2002, Annual review of biomedical engineering.

[8]  O. Guenat,et al.  High-density electrode array for imaging in vitro electrophysiological activity. , 2005, Biosensors & bioelectronics.

[9]  Ulrich Egert,et al.  Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Estimation of Action Potential Changes from Field Potential Recordings in Multicellular Mouse Cardiac Myocyte Cultures Key Words Microelectrode Array @bullet Action Potential Upstroke @bullet Action P , 2022 .

[10]  D. Schmitt-Landsiedel,et al.  A 128 /spl times/ 128 CMOS bio-sensor array for extracellular recording of neural activity , 2003, 2003 IEEE International Solid-State Circuits Conference, 2003. Digest of Technical Papers. ISSCC..

[11]  T. Berger,et al.  Custom-designed high-density conformal planar multielectrode arrays for brain slice electrophysiology , 2006, Journal of Neuroscience Methods.

[12]  J. Pine.  Recording action potentials from cultured neurons with extracellular microcircuit electrodes , 1980, Journal of Neuroscience Methods.

[13]  Martin Fussenegger,et al.  Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. , 2003, Biotechnology and bioengineering.

[14]  U. Frey,et al.  Single-chip microelectronic system to interface with living cells. , 2007, Biosensors & bioelectronics.

[15]  S. Hafizovic,et al.  CMOS microelectrode array for bidirectional interaction with neuronal networks , 2006, IEEE Journal of Solid-State Circuits.

[16]  S. Hafizovic,et al.  11’000 Electrode-, 126 channel-CMOS microelectrode array for electrogenic cells , 2007, 2007 IEEE 20th International Conference on Micro Electro Mechanical Systems (MEMS).

[17]  Steven P Jones,et al.  Functional Integration of Electrically Active Cardiac Derivatives From Genetically Engineered Human Embryonic Stem Cells With Quiescent Recipient Ventricular Cardiomyocytes: Insights Into the Development of Cell-Based Pacemakers , 2005, Circulation.

[18]  Andreas Hierlemann,et al.  An 11k-Electrode 126-Channel High-Density Microelectrode Array to Interact with Electrogenic Cells , 2007, 2007 IEEE International Solid-State Circuits Conference. Digest of Technical Papers.