Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome

BackgroundCentrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.AimDetermine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses.MethodsNuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.ResultsBesides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.ConclusionAlready minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.

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