Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells

Statement of the Problem: The stimulation of odontogenic activity is considered an essential property for biomaterials used in vital pulp therapy. Purpose: The present study aimed to evaluate the effect of the incorporation of zeolite containing silver-zinc nanoparticles (Ze-Ag-Zn) into Angelous mineral trioxide aggregate (AMTA) on the odontogenic activity of human dental pulp stem cells (HDPSCs). Materials and Method: In this in vitro study, HDPSCs were treated with 2% wt of synthesized Ze-Ag-Zn particles+AMTA, AMTA and Ze-Ag-Zn disks. The negative control cells did not receive any treatment. Then, cell viability was measured using the MTT assay after 7 and 14 days of the treatment course. The alkaline phosphatase (ALP) activity and calcium ion level were also measured in the supernatant culture media using auto-analyzer kits. The obtained data were analyzed using one-way ANOVA and Student t-test where appropriate. The level of the statistical significance was set at p< 0.05. Results: The results indicated that HDPSCs treated with AMTA and AMTA+Ze-Ag-Zn particles did not show any significant cell death compared with the control cells after 14 days of the treatment course while the ALP activity and calcium ion levels were significantly (p< 0.05) elevated. Also, the addition of AMTA particles to the cell culture media resulted in increased ALP activity and calcium ion level compared with HDPSCs treated with AMTA+ Ze-Ag-Zn particles on day 7 of the treatment course (p< 0.05). Conclusion: It seems that the incorporation of Ze-Ag-Zn particles into AMTA did not have any significant positive effect on the biomineralization properties of AMTA.

[1]  M. Samiei,et al.  Effect of the of Zeolite Containing Silver-Zinc Nanoparticles on the Push out Bond Strength of Mineral Trioxide Aggregate in Simulated Furcation Perforation , 2019, Journal of dentistry.

[2]  R. Rahbarghazi,et al.  In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components , 2019, Journal of dental research, dental clinics, dental prospects.

[3]  Sanjeet Kumar,et al.  Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method. , 2018, Microbial pathogenesis.

[4]  Ivan Jirka,et al.  Applications of zeolites in biotechnology and medicine - a review. , 2018, Biomaterials science.

[5]  M. Samiei,et al.  Zeolite-silver-zinc nanoparticles: Biocompatibility and their effect on the compressive strength of mineral trioxide aggregate , 2017, Journal of clinical and experimental dentistry.

[6]  M. Samiei,et al.  Effect of the TiO2 nanoparticles on the selected physical properties of mineral trioxide aggregate , 2017, Journal of clinical and experimental dentistry.

[7]  M. Iino,et al.  Eluted zinc ions stimulate osteoblast differentiation and mineralization in human dental pulp stem cells for bone tissue engineering. , 2016, Archives of oral biology.

[8]  V. Zand,et al.  Tissue Reaction and Biocompatibility of Implanted Mineral Trioxide Aggregate with Silver Nanoparticles in a Rat Model , 2015, Iranian endodontic journal.

[9]  M. Samiei,et al.  Nanoparticles for antimicrobial purposes in Endodontics: A systematic review of in vitro studies. , 2016, Materials science & engineering. C, Materials for biological applications.

[10]  K. Yeung,et al.  Silver nanoparticles promote osteogenesis of mesenchymal stem cells and improve bone fracture healing in osteogenesis mechanism mouse model. , 2015, Nanomedicine : nanotechnology, biology, and medicine.

[11]  Anushre Prasad,et al.  A comparative evaluation of the effect of various additives on selected physical properties of white mineral trioxide aggregate , 2015, Journal of conservative dentistry : JCD.

[12]  Foroutan Tahereh.  THE EFFECTS OF ZINC OXIDE NANOPARTICLES ON DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS TO OSTEOBLAST , 2014 .

[13]  Christian Hannig,et al.  Dentin-like tissue formation and biomineralization by multicellular human pulp cell spheres in vitro , 2014, Head & Face Medicine.

[14]  M. Gürel,et al.  The effects of incorporation of silver-zeolite on selected properties of mineral trioxide aggregate. , 2013, Dental materials journal.

[15]  T. Srikhirin,et al.  Antimicrobial effects of silver zeolite, silver zirconium phosphate silicate and silver zirconium phosphate against oral microorganisms. , 2013, Asian Pacific journal of tropical biomedicine.

[16]  H. Yücel,et al.  Short-term antimicrobial properties of mineral trioxide aggregate with incorporated silver-zeolite. , 2011, Dental traumatology : official publication of International Association for Dental Traumatology.

[17]  M. Torabinejad,et al.  Mineral trioxide aggregate: a comprehensive literature review--Part III: Clinical applications, drawbacks, and mechanism of action. , 2010, Journal of endodontics.

[18]  M. Torabinejad,et al.  Mineral trioxide aggregate: a comprehensive literature review--part II: leakage and biocompatibility investigations. , 2010, Journal of endodontics.

[19]  Deepthy Menon,et al.  Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells , 2009, Journal of materials science. Materials in medicine.

[20]  M. Irani,et al.  Preparation and characterization of nanosized ZSM-5 zeolite using kaolin and investigation of kaolin content, crystallization time and temperature changes on the size and crystallinity of products , 2009 .

[21]  Thomas J Webster,et al.  Increased osteoblast and decreased Staphylococcus epidermidis functions on nanophase ZnO and TiO2. , 2006, Journal of biomedical materials research. Part A.

[22]  S. Friedman,et al.  The hemolytic and cytotoxic properties of a zeolite-containing root filling material in vitro. , 2003, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[23]  A. Boyde,et al.  Stem Cell Properties of Human Dental Pulp Stem Cells , 2002, Journal of dental research.