Green Chemistry Glucose Biosensor Development using Etlingera elatior Extract

Glucose biosensor development is one of the important strategies for early detection of diabetes mellitus disease. This study was aimed to explore the flower extract of Etlingera elatior for a green-analysis method of glucose biosensor. Flowers were extracted using ethanol: HCl and tested its performances as an indicator of glucose biosensor using glucose oxidase enzyme. The glucose oxidase react with glucose resulted hydrogen peroxide that would change the color of the flower extract. Furthermore, the extract was also studied including their stability to pH, oxidizing and reducing, temperature, and storage. The results showed that the Etlingera elatior extract had high correlation between color change and glucose concentration with regression equation of y = −0.0005x + 0.4724 and R2 of 0.9965. The studied biosensor showed a wide linear range to detect glucose sample of 0 to 500 mM. The extract characterization showed a more stable in low pH (acid), reducing agent addition, heating treatment and storage.

[1]  S. Wild,et al.  Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. , 2004, Diabetes care.

[2]  K. Khunti,et al.  Waiting for diabetes: perceptions of people with pre-diabetes: a qualitative study. , 2008, Patient education and counseling.

[3]  F. Finger,et al.  Effect of pH and temperature on peroxidase and polyphenoloxidase activities of litchi pericarp , 2010 .

[4]  M. Maaza,et al.  Physical properties of CdO nanoparticles synthesized by green chemistry via Hibiscus Sabdariffa flower extract , 2016 .

[5]  M. J. Cocero,et al.  Thermal degradation of grape marc polyphenols. , 2014, Food chemistry.

[6]  D. Hermawan,et al.  Alginate cryogel based glucose biosensor , 2016 .

[7]  J. Simon,et al.  Recent Advances in Anthocyanin Analysis and Characterization. , 2008, Current analytical chemistry.

[8]  M. D. Anggraeni,et al.  Simple colorimetric glucose biosensor using chitosan cryogel supporting material , 2016 .

[9]  M. Heinonen,et al.  Anthocyanin color behavior and stability during storage: effect of intermolecular copigmentation. , 2002, Journal of agricultural and food chemistry.

[10]  Marek Tobiszewski,et al.  Green analytical chemistry in sample preparation for determination of trace organic pollutants , 2009 .

[11]  A. M. Brett,et al.  Redox Behavior of Anthocyanins Present in Vitis vinifera L. , 2007 .

[12]  Venkatasubramanian Sivakumar,et al.  Ultrasound assisted enhancement in natural dye extraction from beetroot for industrial applications and natural dyeing of leather. , 2009, Ultrasonics sonochemistry.

[13]  P. Kanatharana,et al.  A highly stable oxygen-independent glucose biosensor based on a chitosan-albumin cryogel incorporated with carbon nanotubes and ferrocene , 2013 .

[14]  Sergio Riva,et al.  Laccases: blue enzymes for green chemistry. , 2006, Trends in biotechnology.

[15]  Jacek Namieśnik,et al.  The 12 principles of green analytical chemistry and the SIGNIFICANCE mnemonic of green analytical practices , 2013 .

[16]  G. Ren,et al.  Identification of anthocyanins isolated from black rice (Oryza sativa L.) and their degradation kinetics , 2013 .

[17]  J. Harborne.  Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis , 1973 .

[18]  S. Moh,et al.  The effects of temperature and pH on stability of anthocyanins from red sorghum (Sorghum bicolor) bran , 2012 .