Reconfigurable FET Biosensor for a Wide Detection Range and Electrostatically Tunable Sensing Response

The nanoscale field effect transistors have emerged as promising candidates for highly sensitive bio/chemical detection. Despite the massive progress, current FET biosensing technologies have limitations in terms of adaptability, versatility and throughput. Herein, we present an approach to dynamically tune the sensing performance of a FET biosensor by local electrostatic gating of the metal-semiconductor junctions. Depending upon the bias applied at the local gates, the device switches its operation configurations to deliver functionalities with markedly different sensing characteristics. Such reconfigurability at the runtime allows application suitability on the go, a feature non-existent in the state-of-art FET biosensors. The results of numerical simulations, based on framework validated against the experimental results, reveal promising sensing performance in terms of a high detection sensitivity (~105) with 104-fold tunable sensitivity window and a wide overall dynamic range spanning >4 orders of magnitude using a single device (a ~100-fold improvement over a conventional FET biosensor). The wide dynamic range is also established through a simple analytical approach based on 1:1 binding kinetics for antibody-antigen system. Taken together, the results strongly suggest that the proposed sensor design, representing the first approach of purely electrostatic phenomenon based tuning of sensing performance, has a considerable potential to enable the future development of more compact, multifunctional and adaptive biosensing platforms.

[1]  Mark A. Reed,et al.  Silicon Nanowire Field-Effect Transistors—A Versatile Class of Potentiometric Nanobiosensors , 2015, IEEE Access.

[2]  M. J. Kumar,et al.  Thin Capacitively-Coupled Thyristor as an Ultrasensitive Label-Free Nanogap Biosensor: Proposal and Investigation , 2017, IEEE Sensors Letters.

[3]  David R. Liu,et al.  Analytical Devices Based on Direct Synthesis of DNA on Paper. , 2016, Analytical chemistry.

[4]  Diego A. Oyarzún,et al.  Fundamental Design Principles for Transcription-Factor-Based Metabolite Biosensors. , 2017, ACS synthetic biology.

[5]  Yang‐Kyu Choi,et al.  A pH sensor with a double-gate silicon nanowire field-effect transistor , 2013 .

[6]  Gaetano Scamarcio,et al.  Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor , 2019, Analytical and Bioanalytical Chemistry.

[7]  Stefan Slesazeck,et al.  Reconfigurable silicon nanowire transistors. , 2012, Nano letters.

[8]  G. De Micheli,et al.  Polarity control in double-gate, gate-all-around vertically stacked silicon nanowire FETs , 2012, 2012 International Electron Devices Meeting.

[9]  S. Vitusevich,et al.  Origin of noise in liquid-gated Si nanowire troponin biosensors , 2018, Nanotechnology.

[10]  Yuelin Wang,et al.  Robust ultrasensitive tunneling-FET biosensor for point-of-care diagnostics , 2016, Scientific Reports.

[11]  V. Hytönen,et al.  Neutralized chimeric avidin binding at a reference biosensor surface. , 2015, Langmuir : the ACS journal of surfaces and colloids.

[12]  A. Ionescu,et al.  Electrical characterization of high performance, liquid gated vertically stacked SiNW-based 3D FET biosensors , 2014 .

[13]  M. Meyyappan,et al.  Comparative Study of Field Effect Transistor Based Biosensors , 2016, IEEE Transactions on Nanotechnology.

[14]  C. Lieber,et al.  Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species , 2001, Science.

[15]  M. J. Kumar,et al.  Dielectric modulated overlapping gate-on-drain tunnel-FET as a label-free biosensor , 2015 .

[16]  M. Jagadesh Kumar,et al.  Dielectric-Modulated Impact-Ionization MOS Transistor as a Label-Free Biosensor , 2013, IEEE Electron Device Letters.

[17]  Yang‐Kyu Choi,et al.  Surface engineering for enhancement of sensitivity in an underlap-FET biosensor by control of wettability. , 2013, Biosensors & bioelectronics.

[18]  M. D. Rooij,et al.  Electrochemical Methods: Fundamentals and Applications , 2003 .

[19]  S. Saurabh,et al.  Fundamentals of Tunnel Field-Effect Transistors , 2016 .

[20]  P. Sheehan,et al.  Detection limits for nanoscale biosensors. , 2005, Nano letters.

[21]  M. Meyyappan,et al.  Silicon nanowire biosensors for detection of cardiac troponin I (cTnI) with high sensitivity. , 2016, Biosensors & bioelectronics.

[22]  Fred J Sigworth,et al.  Importance of the Debye screening length on nanowire field effect transistor sensors. , 2007, Nano letters.

[23]  D. Janes,et al.  Low-Frequency Noise Contributions From Channel and Contacts in InAs Nanowire Transistors , 2013, IEEE Transactions on Electron Devices.

[24]  Jörg Opitz,et al.  Schottky barrier-based silicon nanowire pH sensor with live sensitivity control , 2014, Nano Research.

[25]  C. O. Chui,et al.  Channel length dependent sensitivity of Schottky contacted silicon nanowire field-effect transistor sensors , 2012 .

[26]  J. Colinge.  Silicon-on-Insulator Technology: Materials to VLSI , 1991 .

[27]  S. Hammer,et al.  Antiretroviral therapy for HIV infection in 1997. Updated recommendations of the International AIDS Society-USA panel. , 1998, JAMA.

[28]  O. Andersson,et al.  A multiple-ligand approach to extending the dynamic range of analyte quantification in protein microarrays. , 2009, Biosensors & bioelectronics.

[29]  G. Shalev,et al.  Electrostatic Limit of Detection of Nanowire-Based Sensors. , 2015, Small.

[30]  Sung-Jin Choi,et al.  TCAD-Based Simulation Method for the Electrolyte–Insulator–Semiconductor Field-Effect Transistor , 2015, IEEE Transactions on Electron Devices.

[31]  Ashley J. Driscoll,et al.  Numerical modeling of analyte diffusion and adsorption behavior in microparticle and nanoparticle based biosensors , 2018 .

[32]  Kevin W Plaxco,et al.  Engineering biosensors with extended, narrowed, or arbitrarily edited dynamic range. , 2012, Journal of the American Chemical Society.

[33]  M. J. Deen,et al.  Model for the field effect from layers of biological macromolecules on the gates of metal-oxide-semiconductor transistors , 2005 .

[34]  M. Heller,et al.  Active microelectronic chip devices which utilize controlled electrophoretic fields for multiplex DNA hybridization and other genomic applications , 2000, Electrophoresis.

[35]  Electrical detection of the biological interaction of a charged peptide via gallium arsenide junction-field-effect transistors. , 2008, Journal of applied physics.

[36]  S. Li,et al.  Electrical Characterization of Silicon-On-Insulator Materials and Devices , 1995 .

[37]  Charles M. Lieber,et al.  Subthreshold regime has the optimal sensitivity for nanowire FET biosensors. , 2010, Nano letters.

[38]  Clemens Heitzinger,et al.  Optimal design of nanowire field-effect troponin sensors , 2017, Comput. Biol. Medicine.

[39]  Sung Min Seo,et al.  Simulation study on discrete charge effects of SiNW biosensors according to bound target position using a 3D TCAD simulator , 2012, Nanotechnology.

[40]  Muhammad A. Alam,et al.  Screening-limited response of nanobiosensors. , 2007, Nano letters.

[41]  Pratyush Pandey,et al.  Tunnel field-effect transistors (TFET) : modelling and simulation , 2016 .

[42]  M. Reed,et al.  Predictive simulations and optimization of nanowire field-effect PSA sensors including screening , 2013, Nanotechnology.

[43]  A. Dhawan,et al.  Schottky Barrier FET Biosensor for Dual Polarity Detection: A Simulation Study , 2017, IEEE Electron Device Letters.

[44]  P. J. Conroy,et al.  Cardiac troponin I: a case study in rational antibody design for human diagnostics. , 2012, Protein engineering, design & selection : PEDS.

[45]  Anuj Dhawan,et al.  Dielectric-Modulated Field Effect Transistors for DNA Detection: Impact of DNA Orientation , 2016, IEEE Electron Device Letters.

[46]  S. McLaughlin.  Electrostatic Potentials at Membrane-Solution Interfaces , 1977 .

[47]  Dual signal amplification strategy for high-sensitivity detection of copper species in bio-samples with a tunable dynamic range. , 2018, Chemical communications.

[48]  Luca Selmi,et al.  Models for the use of commercial TCAD in the analysis of silicon-based integrated biosensors , 2014 .

[49]  Chi On Chui,et al.  Optimization of the Sensitivity of FET-Based Biosensors via Biasing and Surface Charge Engineering , 2012, IEEE Transactions on Electron Devices.

[50]  M. Jamal Deen,et al.  Study of the electrolyte-insulator-semiconductor field-effect transistor (EISFET) with applications in biosensor design , 2007, Microelectron. Reliab..

[51]  Lidan You,et al.  Effect of nanowire number, diameter, and doping density on nano-FET biosensor sensitivity. , 2011, ACS nano.

[52]  Yi Lu,et al.  Abasic site-containing DNAzyme and aptamer for label-free fluorescent detection of Pb(2+) and adenosine with high sensitivity, selectivity, and tunable dynamic range. , 2009, Journal of the American Chemical Society.

[53]  Yang‐Kyu Choi,et al.  CRP detection from serum for chip-based point-of-care testing system. , 2013, Biosensors & bioelectronics.