Hydrodynamic spinning of hydrogel fibers.

Hydrogel scaffolds are highly hydrated polymer networks that allow cells to adhere, proliferate and differentiate in the treatment of diseased or injured tissues and organs. Using hydrodynamic shaping and in situ cross-linking of hydrogel precursors, we have developed a highly efficient "hydrodynamic spinning" approach for synthesizing hydrogel fibers of different diameters in a multiphase coaxial flow. A triple-orifice spinneret has been created, and three different types of hydrogel precursors have been examined. Without changing the spinning head, hollow and solid hydrogel fibers with different diameters have been spun by simply manipulating the ratio of input flow rates. Together with the ability of simultaneous cell-seeding in the hydrogel matrix, hydrodynamic spinning can be broadly applied to many hydrogel materials, providing a powerful technique in the preparation of fiber-like and tubule-like hydrogel constructs for tissue engineering.

[1]  Andreas Lendlein,et al.  Preparation of highly asymmetric hollow fiber membranes from poly(ether imide) by a modified dry–wet phase inversion technique using a triple spinneret , 2005 .

[2]  Robert Langer,et al.  Biodegradable Polymer Scaffolds for Tissue Engineering , 1994, Bio/Technology.

[3]  J. Vacanti,et al.  Tissue engineering. , 1993, Science.

[4]  Y. Nakayama.  Introduction to fluid mechanics , 1997 .

[5]  Glenn D Prestwich,et al.  Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds. , 2006, Biomaterials.

[6]  E Bell,et al.  A blood vessel model constructed from collagen and cultured vascular cells. , 1986, Science.

[7]  J. Lewis,et al.  Microperiodic structures: Direct writing of three-dimensional webs , 2004, Nature.

[8]  Sangeeta N Bhatia,et al.  Three-dimensional tissue fabrication. , 2004, Advanced drug delivery reviews.

[9]  Yuan Ji,et al.  Dual-syringe reactive electrospinning of cross-linked hyaluronic acid hydrogel nanofibers for tissue engineering applications. , 2006, Macromolecular bioscience.

[10]  Sidney Loeb,et al.  Sea Water Demineralization by Means of an Osmotic Membrane , 1963 .

[11]  Molly S. Shoichet,et al.  Three-dimensional Chemical Patterning of Transparent Hydrogels , 2008 .

[12]  S. Petit-Zeman,et al.  Regenerative medicine , 2001, Nature Biotechnology.

[13]  Buddy D Ratner,et al.  Photo-patterning of porous hydrogels for tissue engineering. , 2007, Biomaterials.

[14]  Rivelino Montenegro,et al.  Chitin-based tubes for tissue engineering in the nervous system. , 2005, Biomaterials.

[15]  Ali Khademhosseini,et al.  Micromolding of shape-controlled, harvestable cell-laden hydrogels. , 2006, Biomaterials.

[16]  Xiaofeng Cui,et al.  Application of inkjet printing to tissue engineering , 2006, Biotechnology journal.

[17]  Wouter Olthuis,et al.  An efficient method for the fabrication of temperature-sensitive hydrogel microactuators. , 2004, Lab on a chip.

[18]  J. Ying,et al.  Controlled formation of biological tubule systems in extracellular matrix gels in vitro. , 2008, Kidney international.

[19]  J. Ying,et al.  Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers. , 2009, Biomaterials.

[20]  Hongkai Wu,et al.  Generation of alginate microfibers with a roller-assisted microfluidic system. , 2009, Lab on a chip.

[21]  D. Mooney,et al.  Hydrogels for tissue engineering: scaffold design variables and applications. , 2003, Biomaterials.

[22]  Thomas Boland,et al.  Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography. , 2004, Tissue engineering.

[23]  K. Nelson,et al.  Release of bovine serum albumin from a hydrogel‐cored biodegradable polymer fiber , 2006, Biopolymers.

[24]  Ying Luo,et al.  A photolabile hydrogel for guided three-dimensional cell growth and migration , 2004, Nature materials.

[25]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[26]  W. Hennink,et al.  Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. , 2007, Tissue engineering.

[27]  Y. Tabata,et al.  Acceleration of Aneurysm Healing by Hollow Fiber Enabling the Controlled Release of Basic Fibroblast Growth Factor , 2006, Neurosurgery.

[28]  Anthony S Weiss,et al.  Electrospun protein fibers as matrices for tissue engineering. , 2005, Biomaterials.

[29]  Ali Khademhosseini,et al.  Microengineered hydrogels for tissue engineering. , 2007, Biomaterials.

[30]  M. Shoichet,et al.  Creating porous tubes by centrifugal forces for soft tissue application. , 2001, Biomaterials.

[31]  J. Fisher,et al.  Photoinitiated Polymerization of Biomaterials , 2001 .

[32]  Christine E Schmidt,et al.  Neural tissue engineering: strategies for repair and regeneration. , 2003, Annual review of biomedical engineering.

[33]  Nuno M Neves,et al.  Electrospun nanostructured scaffolds for tissue engineering applications. , 2007, Nanomedicine.

[34]  A. Atala.  Engineering tissues, organs and cells , 2007, Journal of tissue engineering and regenerative medicine.

[35]  David J Mooney,et al.  Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution. , 2005, Biomaterials.

[36]  A. Hoffman.  Hydrogels for Biomedical Applications , 2001, Advanced drug delivery reviews.

[37]  Takehisa Matsuda,et al.  Preparation of vinylated polysaccharides and photofabrication of tubular scaffolds as potential use in tissue engineering. , 2002, Biomacromolecules.

[38]  G. Whitesides,et al.  Experimental and theoretical scaling laws for transverse diffusive broadening in two-phase laminar flows in microchannels , 2000 .

[39]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. , 2002, Tissue engineering.

[40]  Robert T Tranquillo.  The Tissue‐Engineered Small‐Diameter Artery , 2002, Annals of the New York Academy of Sciences.

[41]  P. Vos,et al.  Cell encapsulation: Promise and progress , 2003, Nature Medicine.

[42]  David J Beebe,et al.  Hydrodynamic microfabrication via"on the fly" photopolymerization of microscale fibers and tubes. , 2004, Lab on a chip.

[43]  Robert F. Shepherd,et al.  Direct‐Write Assembly of 3D Hydrogel Scaffolds for Guided Cell Growth , 2009 .

[44]  Su-Jung Shin,et al.  "On the fly" continuous generation of alginate fibers using a microfluidic device. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[45]  K. Anseth,et al.  Hydrogel Cell Cultures , 2007, Science.