Chronic quercetin ingestion and exercise-induced oxidative damage and inflammation.

Quercetin is a flavonoid compound that has been demonstrated to be a potent antioxidant in vitro. The objective of this study was to evaluate if quercetin ingestion would increase plasma antioxidant measures and attenuate increases in exercise-induced oxidative damage. Forty athletes were recruited and randomized to quercetin or placebo. Subjects consumed 1000 mg quercetin or placebo each day for 6 weeks before and during 3 d of cycling at 57% work maximum for 3 h. Blood was collected before and immediately after exercise each day, and analyzed for F2-isoprostanes, nitrite, ferric-reducing ability of plasma, trolox equivalent antioxidant capacity, and C-reactive protein. Statistical analyses involved a 2 (treatment) x 6 (times) repeated measures analysis of variance to test main effects. F2-isoprostanes, nitrite, ferric-reducing ability of plasma, trolox equivalent antioxidant capacity, and C-reactive protein were significantly elevated as a result of exercise, but no group effects were found. Despite previous data demonstrating potent antioxidant actions of quercetin in vitro, this study indicates that this effect is absent in vivo and that chronic quercetin ingestion does not exert protection from exercise-induced oxidative stress and inflammation.

[1]  C. Rice-Evans,et al.  Cellular uptake and metabolism of flavonoids and their metabolites: implications for their bioactivity. , 2004, Archives of biochemistry and biophysics.

[2]  J. Morrow,et al.  Mass spectrometric quantification of F2-isoprostanes in biological fluids and tissues as measure of oxidant stress. , 1999, Methods in enzymology.

[3]  J. Morrow,et al.  Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise. , 2005, The Journal of nutritional biochemistry.

[4]  R. Prior,et al.  Comparison of different analytical methods for assessing total antioxidant capacity of human serum. , 1998, Clinical chemistry.

[5]  O. Texier,et al.  Quercetin is recovered in human plasma as conjugated derivatives which retain antioxidant properties , 1998, FEBS letters.

[6]  J. Morrow,et al.  Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. , 2004, Free radical biology & medicine.

[7]  D. Nieman,et al.  Effects of quercetin and EGCG on mitochondrial biogenesis and immunity. , 2009, Medicine and science in sports and exercise.

[8]  J. Morrow,et al.  Influence of Carbohydrate Ingestion on Oxidative Stress and Plasma Antioxidant Potential Following a 3 h Run , 2003, Free radical research.

[9]  L. Goya,et al.  Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. , 2006, Toxicology and applied pharmacology.

[10]  H. Yanai,et al.  Effect of ascorbate on serum lipids and urate metabolism during exhaustive training. , 2004, Clinical science.

[11]  S. Powers,et al.  Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. , 1993, The American journal of physiology.

[12]  U. Takahama,et al.  Quercetin-dependent inhibition of nitration induced by peroxidase/H2O2/nitrite systems in human saliva and characterization of an oxidation product of quercetin formed during the inhibition. , 2005, Journal of agricultural and food chemistry.

[13]  S. Hannum.  Potential Impact of Strawberries on Human Health: A Review of the Science , 2004, Critical reviews in food science and nutrition.

[14]  C. Rice-Evans,et al.  Inhibition of peroxynitrite-mediated tyrosine nitration by catechin polyphenols. , 1997, Biochemical and biophysical research communications.

[15]  Peter Johnson,et al.  Antioxidant enzyme expression in health and disease: effects of exercise and hypertension. , 2002, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[16]  T. Sanders,et al.  Effect of dietary quercetin on oxidative DNA damage in healthy human subjects. , 2000, The British journal of nutrition.

[17]  S. Powers,et al.  Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review. , 1999, Medicine and science in sports and exercise.

[18]  A. Agrawal,et al.  Effect of wine phenolics on cytokine‐induced C‐reactive protein expression , 2007, Journal of thrombosis and haemostasis : JTH.

[19]  Ľ. Heilerová,et al.  Evaluation of the redox properties and anti/pro-oxidant effects of selected flavonoids by means of a DNA-based electrochemical biosensor , 2003, Analytical and bioanalytical chemistry.

[20]  J. Kleinjans,et al.  Protection by quercetin and quercetin-rich fruit juice against induction of oxidative DNA damage and formation of BPDE-DNA adducts in human lymphocytes. , 2005, Mutation research.

[21]  A. Nanji,et al.  NF-κB ACTIVATION IS ASSOCIATED WITH FREE RADICAL GENERATION AND ENDOTOXEMIA AND PRECEDES PATHOLOGICAL LIVER INJURY IN EXPERIMENTAL ALCOHOLIC LIVER DISEASE , 2001 .

[22]  R. Tyrrell,et al.  Exercise-induced expression of heme oxygenase-1 in human lymphocytes , 2005, Free radical research.

[23]  V. Dobson,et al.  Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study , 2000, European Journal of Clinical Nutrition.

[24]  Gary Williamson,et al.  Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. , 2005, The American journal of clinical nutrition.

[25]  Gary Williamson,et al.  Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention studies. , 2005, The American journal of clinical nutrition.

[26]  S. Bischoff.  Quercetin: potentials in the prevention and therapy of disease , 2008, Current opinion in clinical nutrition and metabolic care.

[27]  Ana M. Troncoso,et al.  Comparison of antioxidant activity of wine phenolic compounds and metabolites in vitro , 2005 .

[28]  D. Costill,et al.  Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. , 1974, Journal of applied physiology.

[29]  Joseph Rafter,et al.  Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not? , 2005, The American journal of clinical nutrition.

[30]  A. Bast,et al.  Masking of antioxidant capacity by the interaction of flavonoids with protein. , 2001, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[31]  S. Phinney,et al.  A dietary supplement attenuates IL-6 and CRP after eccentric exercise in untrained males. , 2003, Medicine and science in sports and exercise.

[32]  S. Basu,et al.  Factors regulating isoprostane formation in vivo. , 2005, Antioxidants & redox signaling.

[33]  R. Griffiths,et al.  Contractile activity-induced oxidative stress: cellular origin and adaptive responses. , 2001, American journal of physiology. Cell physiology.

[34]  C. Rice-Evans,et al.  Flavonoids: antioxidants or signalling molecules? , 2004, Free radical biology & medicine.

[35]  J. González‐Gallego,et al.  Changes in oxidative stress markers and NF-κB activation induced by sprint exercise , 2005, Free radical research.

[36]  P. Hollman,et al.  Absorption, metabolism and health effects of dietary flavonoids in man. , 1997, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[37]  M. Ackland,et al.  Synergistic antiproliferative action of the flavonols quercetin and kaempferol in cultured human cancer cell lines. , 2005, In vivo.

[38]  J. González‐Gallego,et al.  Quercetin Attenuates Nuclear Factor-κB Activation and Nitric Oxide Production in Interleukin-1β–Activated Rat Hepatocytes , 2005 .

[39]  J. Tur,et al.  Antioxidant response to oxidative stress induced by exhaustive exercise , 2005, Physiology & Behavior.

[40]  J. González‐Gallego,et al.  The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2 and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang Liver cells. , 2007, European journal of pharmacology.

[41]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[42]  M. Aviram,et al.  Wine Flavonoids Protect against LDL Oxidation and Atherosclerosis , 2002, Annals of the New York Academy of Sciences.

[43]  A. Rousseau,et al.  Does physical exercise modify antioxidant requirements? , 2008, Nutrition Research Reviews.

[44]  Kelly E Heim,et al.  Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. , 2002, The Journal of nutritional biochemistry.

[45]  O. Texier,et al.  Bioavailability of rutin and quercetin in rats , 1997, FEBS letters.

[46]  A. L'Abbate,et al.  Determination of Nitrite Plus Nitrate and Malondialdehyde in Human Plasma: Analytical Performance and the Effect of Smoking and Exercise , 2002, Clinical chemistry and laboratory medicine.

[47]  H. Macrae,et al.  Dietary antioxidant supplementation combined with quercetin improves cycling time trial performance. , 2006, International journal of sport nutrition and exercise metabolism.

[48]  A. Utter,et al.  Quercetin's effect on cycling efficiency and substrate utilization. , 2009, Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme.

[49]  J. Morrow.  Quantification of Isoprostanes as Indices of Oxidant Stress and the Risk of Atherosclerosis in Humans , 2004, Arteriosclerosis, thrombosis, and vascular biology.