论文信息 - Experimental techniques of two-dimensional correlated spectroscopy

Experimental techniques of two-dimensional correlated spectroscopy

Abstract Two-dimensional (2D) homonuclear correlated spectra manifest connectivities between spin-coupled nuclei and can thus provide assignments of individual spin systems in complex 1 H NMR spectra. Two experimental techniques discussed in this paper, spin-echo correlated spectroscopy and foldover-corrected correlated spectroscopy, are particularly suitable versions for handling the large data matrices encountered in work with biological macromolecules. This paper explains the fundamental aspects of these two techniques and the relations with the conventional 2D correlated spectroscopy technique.

[1] Anil Kumar. Two-dimensional spin-echo NMR spectroscopy: A general method for calculation of spectra , 1978 .

[2] K Wüthrich,et al. Two-dimensional J-resolved 1H n.m.r. spectroscopy for studies of biological macromolecules. , 1977, Biochemical and biophysical research communications.

[3] K. W. Cattermole. The Fourier Transform and its Applications , 1965 .

[4] R. R. Ernst,et al. Phase separation in two-dimensional spectroscopy , 1977 .

[5] Richard R. Ernst,et al. Homonuclear broad band decoupling and two-dimensional J-resolved NMR spectroscopy , 1976 .

[6] D. C. Champeney,et al. Fourier Transforms and Their Physical Applications , 1973 .

[7] K. Wüthrich,et al. Individual assignments of the methyl resonances in the 1H nuclear magnetic resonance spectrum of the basic pancreatic trypsin inhibitor. , 1978, Biochemistry.

[8] K. Wüthrich,et al. Two-dimensional spin echo correlated spectroscopy (SECSY) for 1H NMR studies of biological macromolecules. , 1979, Biochemical and biophysical research communications.

[9] High-sensitivity two-dimensional carbon-13 NMR spectroscopy , 1979 .

[10] R. R. Ernst,et al. Two‐dimensional spectroscopy. Application to nuclear magnetic resonance , 1976 .