Dielectrophoretically Aligned Carbon Nanotubes to Control Electrical and Mechanical Properties of Hydrogels to Fabricate Contractile Muscle Myofibers

Dielectrophoresis is used to align carbon nanotubes (CNTs) within gelatin methacrylate (GelMA) hydrogels in a facile and rapid manner. Aligned GelMA-CNT hydrogels show higher electrical properties compared with pristine and randomly distributed CNTs in GelMA hydrogels. The muscle cells cultured on these materials demonstrate higher maturation compared with cells cultured on pristine and randomly distributed CNTs in GelMA hydrogels.

[1]  F. Rawson,et al.  Tailoring 3D Single-Walled Carbon Nanotubes Anchored to Indium Tin Oxide for Natural Cellular Uptake and Intracellular Sensing , 2012, Nano letters.

[2]  J. Ramón‐Azcón,et al.  Detection of pesticide residues using an immunodevice based on negative dielectrophoresis. , 2009, Biosensors & bioelectronics.

[3]  S. Sirivisoot,et al.  Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds , 2011, International journal of nanomedicine.

[4]  Ali Khademhosseini,et al.  Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells. , 2012, Lab on a chip.

[5]  Ali Khademhosseini,et al.  Directed 3D cell alignment and elongation in microengineered hydrogels. , 2010, Biomaterials.

[6]  H. Bayley,et al.  Lipid-coated hydrogel shapes as components of electrical circuits and mechanical devices , 2012, Scientific Reports.

[7]  P. Caroni,et al.  Muscle LIM protein, a novel essential regulator of myogenesis, promotes myogenic differentiation , 1994, Cell.

[8]  A. Khademhosseini,et al.  Cell-laden microengineered gelatin methacrylate hydrogels. , 2010, Biomaterials.

[9]  H. Choi,et al.  Nanofibrous Membranes Prepared by Multiwalled Carbon Nanotube/Poly(methyl methacrylate) Composites , 2004 .

[10]  Seunghun Hong,et al.  Controlling the growth and differentiation of human mesenchymal stem cells by the arrangement of individual carbon nanotubes. , 2011, ACS nano.

[11]  A. Khademhosseini,et al.  Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation. , 2012, ACS nano.

[12]  B. V. Derjaguin,et al.  Effect of contact deformations on the adhesion of particles , 1975 .

[13]  Tomoyuki Yasukawa,et al.  Competitive multi-immunosensing of pesticides based on the particle manipulation with negative dielectrophoresis. , 2010, Biosensors & bioelectronics.

[14]  Tal Dvir,et al.  Nanotechnological strategies for engineering complex tissues. , 2020, Nature nanotechnology.

[15]  Hui Hu,et al.  Preparation of Single-Walled Carbon Nanotube Reinforced Polystyrene and Polyurethane Nanofibers and Membranes by Electrospinning , 2004 .

[16]  P. Schwille.  Bottom-Up Synthetic Biology: Engineering in a Tinkerer’s World , 2011, Science.

[17]  Ali Khademhosseini,et al.  Interdigitated array of Pt electrodes for electrical stimulation and engineering of aligned muscle tissue. , 2012, Lab on a chip.

[18]  Haihui Ye,et al.  Electrospinning of Continuous Carbon Nanotube‐Filled Nanofiber Yarns , 2003 .

[19]  R. Langer,et al.  The smartest materials: the future of nanoelectronics in medicine. , 2012, ACS nano.

[20]  Charles M. Lieber,et al.  Design and synthesis of diverse functional kinked nanowire structures for nanoelectronic bioprobes. , 2013, Nano letters.

[21]  Ali Khademhosseini,et al.  Engineered contractile skeletal muscle tissue on a microgrooved methacrylated gelatin substrate. , 2012, Tissue engineering. Part A.

[22]  A. Khademhosseini,et al.  Hydrogels in Regenerative Medicine , 2009, Advanced materials.

[23]  A. Khademhosseini,et al.  Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology , 2006 .

[24]  Jin-Oh You,et al.  Nanoengineering the heart: conductive scaffolds enhance connexin 43 expression. , 2011, Nano letters.

[25]  Tal Dvir,et al.  Nanowired three dimensional cardiac patches , 2011, Nature nanotechnology.

[26]  M. Gandhi,et al.  Carbon nanotube reinforced Bombyx mori silk nanofibers by the electrospinning process. , 2006, Biomacromolecules.

[27]  A. Khademhosseini,et al.  Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators. , 2013, ACS nano.

[28]  Ali Khademhosseini,et al.  Electrical stimulation as a biomimicry tool for regulating muscle cell behavior , 2013, Organogenesis.

[29]  Charles M. Lieber,et al.  Macroporous nanowire nanoelectronic scaffolds for synthetic tissues. , 2012, Nature materials.

[30]  Adam J Engler,et al.  Intrinsic extracellular matrix properties regulate stem cell differentiation. , 2010, Journal of biomechanics.

[31]  J. Stegemann,et al.  Directional conductivity in SWNT-collagen-fibrin composite biomaterials through strain-induced matrix alignment. , 2008, Journal of biomedical materials research. Part A.

[32]  Jacob T. Robinson,et al.  Nanowire-Mediated Delivery Enables Functional Interrogation of Primary Immune Cells: Application to the Analysis of Chronic Lymphocytic Leukemia , 2012, Nano letters.

[33]  Matthias P. Lutolf,et al.  Designing materials to direct stem-cell fate , 2009, Nature.

[34]  Eyal Zussman,et al.  Carbon Nanotubes Embedded in Oriented Polymer Nanofibers by Electrospinning , 2003 .

[35]  Nikhil Koratkar,et al.  Viscoelasticity in carbon nanotube composites , 2005, Nature materials.