Evaluation of PCL/chitosan electrospun nanofibers for liver tissue engineering

ABSTRACT In this investigation, a nanofibrous scaffold was fabricated through electrospinning of polycaprolactone (PCL) and chitosan (CS) using a novel collector to make better orientation and pore size for cell infiltration. PCL/CS nanofibers with 90-rpm collector speed and 40° angle between collector wires of the new collector have fewer diameters with better pore, size and nanofibers orientation. Mechanical properties, roughness parameters, topology, structure, hydrophilicity, and cell growing were considered for liver tissue engineering. The cell culture was done using epithelial liver mouse cells. The developed electrospun PCL/CS scaffold using novel collector would be an excellent matrix for biomedical applications especially liver tissue engineering. GRAPHICAL ABSTRACT

[1]  C. Wykes.  Handbook of Surface and Nanometrology, 2nd edn., by David J. Whitehouse , 2012 .

[2]  Nava P. Rijal,et al.  Fabrication and Characterization of Electrospun PCL-MgO-Keratin-Based Composite Nanofibers for Biomedical Applications , 2015, Materials.

[3]  David J. Whitehouse,et al.  Handbook of Surface and Nanometrology, Second Edition , 2011 .

[4]  L. Ghasemi‐Mobarakeh,et al.  A novel method for porosity measurement of various surface layers of nanofibers mat using image analysis for tissue engineering applications , 2007 .

[5]  K J Stout,et al.  Development of methods for the characterisation of roughness in three dimensions , 2000 .

[6]  B. D. Favis,et al.  Poly(ε-caprolactone) scaffolds of highly controlled porosity and interconnectivity derived from co-continuous polymer blends: model bead and cell infiltration behavior , 2014, Journal of Materials Science: Materials in Medicine.

[7]  S. Nair,et al.  Single step electrospinning of chitosan/poly(caprolactone) nanofibers using formic acid/acetone solvent mixture , 2010 .

[8]  D. Hay,et al.  Liver tissue engineering and cell sources: issues and challenges , 2013, Liver international : official journal of the International Association for the Study of the Liver.

[9]  T. Maekawa,et al.  POLYMERIC SCAFFOLDS IN TISSUE ENGINEERING APPLICATION: A REVIEW , 2011 .

[10]  Richard G. Ellenbogen,et al.  Natural‐Synthetic Polyblend Nanofibers for Biomedical Applications , 2009 .

[11]  Antonios G Mikos,et al.  Influence of the porosity of starch-based fiber mesh scaffolds on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in a flow perfusion bioreactor. , 2006, Tissue engineering.

[12]  M. Abedalwafa,et al.  BIODEGRADABLE POLY-EPSILON-CAPROLACTONE (PCL) FOR TISSUE ENGINEERING APPLICATIONS: A REVIEW , 2012 .

[13]  Miqin Zhang,et al.  Fabrication and cellular compatibility of aligned chitosan–PCL fibers for nerve tissue regeneration , 2011 .

[14]  O. Kwon,et al.  Electrospinning of chitosan dissolved in concentrated acetic acid solution. , 2005, Biomaterials.

[15]  S. Bertoldi,et al.  Nano/Micro Hybrid Scaffold of PCL or P3HB Nanofibers Combined with Silk Fibroin for Tendon and Ligament Tissue Engineering , 2014, Journal of applied biomaterials & functional materials.

[16]  Antonios G Mikos,et al.  Biomimetic materials for tissue engineering. , 2003, Biomaterials.

[17]  D. Semnani.  A novel model of optimum nanofibre distribution in nanofibre scaffold structure by genetic algorithm method , 2014 .

[18]  Changyou Gao,et al.  Polycaprolactone scaffold modified with galactosylated chitosan for hepatocyte culture , 2012, Macromolecular Research.

[19]  Dariush Semnani,et al.  A novel method for the determination of cell infiltration into nanofiber scaffolds using image analysis for tissue engineering applications , 2009 .

[20]  David J. Whitehouse,et al.  Handbook of Surface and Nanometrology , 2002 .

[21]  Dariush Semnani,et al.  Silk Fibroin Nano-Coated Textured Silk Yarn by Electrospinning Method for Tendon and Ligament Scaffold Application , 2014 .

[22]  Yong-fang Qian,et al.  Fabrication and characterization of electrospun polycaprolactone blended with chitosan-gelatin complex nanofibrous mats , 2014 .

[23]  Feng Chen,et al.  Preparation and characterization of oxidized alginate covalently cross-linked galactosylated chitosan scaffold for liver tissue engineering , 2012 .

[24]  M. Torbenson,et al.  Chitosan-DNA nanoparticles delivered by intrabiliary infusion enhance liver-targeted gene delivery , 2006, International journal of nanomedicine.

[25]  Forrest M Kievit,et al.  Aligned Chitosan‐Polycaprolactone Polyblend Nanofibers Promote the Migration of Glioblastoma Cells , 2013, Advanced healthcare materials.

[26]  Chaozong Liu,et al.  Design and Development of Three-Dimensional Scaffolds for Tissue Engineering , 2007 .