JCB_200811005 1209..1226

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 185 No. 7 1209–1225 www.jcb.org/cgi/doi/10.1083/jcb.200811005 JCB 1209 C. Kuijl and R. van der Kant contributed equally to this paper. Correspondence to Wilbert Zwart: w.zwart@nki.nl; or Jacques Neefjes: j.neefjes@nki.nl Abbreviations used in this paper: BP, band pass; CLSM, confocal laser-scanning microscopy; DIC, dynein intermediate chain; FLIM, fluorescence lifetime imaging microscopy; FRET, fluorescence resonance energy transfer; IMDM, Iscove’s modified Dulbecco’s medium; LE, late endosome; LUT, look up table; MBP, maltose-binding protein; MCS, membrane contact site; mRFP, monomeric RFP; MTOC, microtubule-organizing center; ORD, OSBP-related domain; OSBP, oxysterol-binding protein; PH, pleckstrin homology; qPCR, quantitative PCR; RILP, Rab7-interacting lysosomal protein; VAP, VAMP-associated ER protein. Introduction

[1]  S. Humbert,et al.  Huntingtin phosphorylation acts as a molecular switch for anterograde/retrograde transport in neurons , 2008, The EMBO journal.

[2]  P. Low,et al.  Cholesterol level regulates endosome motility via Rab proteins. , 2008, Biophysical journal.

[3]  Elina Ikonen,et al.  Cellular cholesterol trafficking and compartmentalization , 2008, Nature Reviews Molecular Cell Biology.

[4]  M. Fivaz,et al.  Late Endosomal Cholesterol Accumulation Leads to Impaired Intra-Endosomal Trafficking , 2007, PloS one.

[5]  C. Hoogenraad,et al.  Motor Neuron Disease-Associated Mutant Vesicle-Associated Membrane Protein-Associated Protein (VAP) B Recruits Wild-Type VAPs into Endoplasmic Reticulum-Derived Tubular Aggregates , 2007, The Journal of Neuroscience.

[6]  V. Olkkonen,et al.  The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket. , 2007, The Biochemical journal.

[7]  W. Zwart,et al.  Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor βlll spectrin , 2007, The Journal of cell biology.

[8]  N. Ohgami,et al.  Roles of Endogenously Synthesized Sterols in the Endocytic Pathway* , 2006, Journal of Biological Chemistry.

[9]  H. Hauri,et al.  Morphogenesis of the Endoplasmic Reticulum: Beyond Active Membrane Expansion , 2006, Traffic.

[10]  浜本 いつき.  Human VAP-B Is Involved in Hepatitis C Virus Replication through Interaction with NS5A and NS5B , 2006 .

[11]  T. Cover,et al.  The oxysterol-binding protein homologue ORP1L interacts with Rab7 and alters functional properties of late endocytic compartments. , 2005, Molecular biology of the cell.

[12]  F. Maxfield,et al.  Role of cholesterol and lipid organization in disease , 2005, Nature.

[13]  I. Vernos,et al.  Kinesin‐2 is a Motor for Late Endosomes and Lysosomes , 2005, Traffic.

[14]  I. Jordens,et al.  Rab Proteins, Connecting Transport and Vesicle Fusion , 2005, Traffic.

[15]  James H. Hurley,et al.  Structural mechanism for sterol sensing and transport by OSBP-related proteins , 2005, Nature.

[16]  S. Walkley,et al.  Protein transduction of Rab9 in Niemann‐Pick C cells reduces cholesterol storage , 2005, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[17]  Bianca Habermann,et al.  Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis , 2005, Nature.

[18]  B. Habermann,et al.  Modulation of Receptor Recycling and Degradation by the Endosomal Kinesin KIF16B , 2005, Cell.

[19]  Christopher J. R. Loewen,et al.  A Highly Conserved Binding Site in Vesicle-associated Membrane Protein-associated Protein (VAP) for the FFAT Motif of Lipid-binding Proteins* , 2005, Journal of Biological Chemistry.

[20]  J. Holthuis,et al.  Lipid traffic: floppy drives and a superhighway , 2005, Nature Reviews Molecular Cell Biology.

[21]  W. Zwart,et al.  Spatial separation of HLA-DM/HLA-DR interactions within MIIC and phagosome-induced immune escape. , 2005, Immunity.

[22]  F. Maxfield,et al.  Lipid and cholesterol trafficking in NPC. , 2004, Biochimica et biophysica acta.

[23]  I. Jordens,et al.  Dynein-mediated vesicle transport controls intracellular Salmonella replication. , 2004, Molecular biology of the cell.

[24]  Kees Jalink,et al.  Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission. , 2004, Biophysical journal.

[25]  J. Hamilton.  Fast flip-flop of cholesterol and fatty acids in membranes: implications for membrane transport proteins. , 2003, Current opinion in lipidology.

[26]  Borivoj Vojnovic,et al.  Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy. , 2003, The Biochemical journal.

[27]  Christopher J. R. Loewen,et al.  A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP , 2003, The EMBO journal.

[28]  I. Vernos,et al.  Dynactin is required for bidirectional organelle transport , 2003, The Journal of cell biology.

[29]  N. Ridgway,et al.  Vesicle-associated Membrane Protein-associated Protein-A (VAP-A) Interacts with the Oxysterol-binding Protein to Modify Export from the Endoplasmic Reticulum* , 2002, The Journal of Biological Chemistry.

[30]  H. Goodson,et al.  Late endosome motility depends on lipids via the small GTPase Rab7 , 2002, The EMBO journal.

[31]  S. Pfeffer,et al.  Rab GTPases: specifying and deciphering organelle identity and function. , 2001, Trends in cell biology.

[32]  Jacques Neefjes,et al.  The Rab7 effector protein RILP controls lysosomal transport by inducing the recruitment of dynein-dynactin motors , 2001, Current Biology.

[33]  Marino Zerial,et al.  Rab proteins as membrane organizers , 2001, Nature Reviews Molecular Cell Biology.

[34]  M. Lemmon,et al.  Signal-dependent membrane targeting by pleckstrin homology (PH) domains. , 2000, The Biochemical journal.

[35]  J. Neefjes,et al.  The major substrates for TAP in vivo are derived from newly synthesized proteins , 2000, Nature.

[36]  C. Echeverri,et al.  Opposing motor activities of dynein and kinesin determine retention and transport of MHC class II-containing compartments. , 1999, Journal of cell science.

[37]  Rainer Pepperkok,et al.  Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy , 1999, Current Biology.

[38]  P. Bastiaens,et al.  Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. , 1999, Trends in cell biology.

[39]  Y. Nishimura,et al.  Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins. , 1999, Biochemical and biophysical research communications.

[40]  L. Liscum,et al.  Evidence for a Cholesterol Transport Pathway from Lysosomes to Endoplasmic Reticulum That Is Independent of the Plasma Membrane* , 1998, The Journal of Biological Chemistry.

[41]  B. Goud,et al.  Interaction of a Golgi-associated kinesin-like protein with Rab6. , 1998, Science.

[42]  C. Echeverri,et al.  Overexpression of the Dynamitin (p50) Subunit of the Dynactin Complex Disrupts Dynein-dependent Maintenance of Membrane Organelle Distribution , 1997, The Journal of cell biology.

[43]  J. Calafat,et al.  Human monocytes and neutrophils store transforming growth factor-alpha in a subpopulation of cytoplasmic granules. , 1997, Blood.

[44]  K. G. Coleman,et al.  Niemann-Pick C1 disease gene: homology to mediators of cholesterol homeostasis. , 1997, Science.

[45]  N. Hirokawa,et al.  Point mutation of adenosine triphosphate-binding motif generated rigor kinesin that selectively blocks anterograde lysosome membrane transport , 1995, The Journal of cell biology.

[46]  M. Brenner,et al.  Interaction with newly synthesized and retained proteins in the endoplasmic reticulum suggests a chaperone function for human integral membrane protein IP90 (calnexin). , 1993, The Journal of biological chemistry.

[47]  R. Vale,et al.  Directional instability of microtubule transport in the presence of kinesin and dynein, two opposite polarity motor proteins , 1992, The Journal of cell biology.

[48]  P. Hollenbeck,et al.  Radial extension of macrophage tubular lysosomes supported by kinesin , 1990, Nature.

[49]  L. Liscum,et al.  The intracellular transport of low density lipoprotein-derived cholesterol is inhibited in Chinese hamster ovary cells cultured with 3-beta-[2-(diethylamino)ethoxy]androst-5-en-17-one. , 1989, The Journal of biological chemistry.

[50]  L. Liscum,et al.  The intracellular transport of low density lipoprotein-derived cholesterol is defective in Niemann-Pick type C fibroblasts , 1989, The Journal of cell biology.

[51]  A. Kolk,et al.  Biochemical characterization and cellular localization of a formalin‐resistant melanoma‐associated antigen reacting with monoclonal antibody NKI/C‐3 , 1985, International journal of cancer.

[52]  H. Brewer,et al.  The ABCA1 Transporter Modulates Late Endocytic Trafficking INSIGHTS FROM THE CORRECTION OF THE GENETIC DEFECT IN TANGIER DISEASE* □ S , 2004 .

[53]  E. Ikonen,et al.  Mobilization of late-endosomal cholesterol is inhibited by Rab guanine nucleotide dissociation inhibitor , 2000, Current Biology.

[54]  R. Palmiter,et al.  Late endosomal membranes rich in lysobisphosphatidic acid regulate cholesterol transport , 1999, Nature Cell Biology.

[55]  R. Brady,et al.  Type C Niemann-Pick disease: use of hydrophobic amines to study defective cholesterol transport. , 1991, Developmental neuroscience.

[56]  L. Haimo,et al.  Lysed chromatophores: a model system for the study of bidirectional organelle transport. , 1989, Methods in cell biology.

[57]  M. Brown,et al.  Receptor-mediated endocytosis of low-density lipoprotein in cultured cells. , 1983, Methods in enzymology.