Enhanced phytoextraction of Cu, Pb, Zn and Cd with EDTA and EDDS.

Chemically enhanced phytoextraction has been proposed as an effective approach to removing heavy metals from contaminated soil through the use of high biomass plants. Using pot experiments, the effects of the application of EDTA, EDDS and citric acid on the uptake of Cu, Pb, Zn and Cd by corn (Zea mays L. cv. Nongda 108) and bean (Phaseolus vulgaris L. white bean) plants were studied. The results showed that EDDS was more effective than EDTA at increasing the concentration of Cu in corn and beans. The application of 5 mmol kg-1 soil EDDS to soil significantly increased concentrations of Cu in shoots, with maximum levels of 2060 and 5130 mg kg-1 DW in corn and beans, respectively, which were 45- and 135-fold higher than that in the corresponding control plants to which chelate had not been applied. Concentrations of Zn in shoots were also higher in the plants treated with EDDS than in those treated with EDTA. For Pb and Cd, EDDS was less effective than EDTA. The maximum Cu phytoextraction was found with the EDDS treatment. The application of EDTA and EDDS also significantly increased the shoot-to-root ratios of the concentrations of Cu, Pb, Zn and Cd in both plant species. The results of metal extraction with chelates showed that EDDS was more efficient at solubilizing Cu and Zn than EDTA, and that EDTA was better at solubilizing Pb and Cd than EDDS.

[1]  S. Gupta,et al.  The role of nitrilotriacetate in copper uptake by tobacco. , 2003, Journal of environmental quality.

[2]  W. R. Berti,et al.  Chelate-assisted phytoextraction of lead from contaminated soils , 1999 .

[3]  S. D. Cunningham,et al.  Chelate-Assisted Pb Phytoextraction: Pb Availability, Uptake, and Translocation Constraints , 1999 .

[4]  Environmental fate and microbial degradation of aminopolycarboxylic acids. , 2001, FEMS microbiology reviews.

[5]  Chi Sun Poon,et al.  Heavy metal contamination of urban soils and street dusts in Hong Kong , 2001 .

[6]  M. B. Kirkham,et al.  EDTA-assisted heavy-metal uptake by poplar and sunflower grown at a long-term sewage-sludge farm , 2003, Plant and Soil.

[7]  Ilya Raskin,et al.  Enhanced Accumulation of Pb in Indian Mustard by Soil-Applied Chelating Agents , 1997 .

[8]  Bernd Nowack,et al.  Extraction of heavy metals from soils using biodegradable chelating agents. , 2004, Environmental science & technology.

[9]  G. Bañuelos,et al.  Phytoremediation of Contaminated Soil and Water , 1999 .

[10]  B. Kos,et al.  Influence of a biodegradable ([S,S]-EDDS) and nondegradable (EDTA) chelate and hydrogel modified soil water sorption capacity on Pb phytoextraction and leaching , 2003, Plant and Soil.

[11]  T C Feijtel,et al.  Environmental risk assessment for trisodium [S,S]-ethylene diamine disuccinate, a biodegradable chelator used in detergent applications. , 1999, Chemosphere.

[12]  Zhenguo Shen,et al.  The copper tolerance mechanisms of Elsholtzia haichowensis, a plant from copper-enriched soils , 2004 .

[13]  P. Römkens,et al.  Potentials and drawbacks of chelate-enhanced phytoremediation of soils. , 2002, Environmental pollution.

[14]  Catherine N. Mulligan,et al.  Remediation technologies for metal-contaminated soils and groundwater: an evaluation , 2001 .

[15]  B. Lothenbach,et al.  The influence of nitrilotriacetate on heavy metal uptake of lettuce and ryegrass , 1999 .

[16]  Alan J. M. Baker,et al.  Metal Hyperaccumulator Plants: A Review of the Ecology and Physiology of a Biological Resource for Phytoremediation of Metal-Polluted Soils , 2000 .

[17]  S. K. Gupta,et al.  Enhancement of phytoextraction of Zn, Cd, and Cu from calcareous soil: The use of NTA and sulfur amendments , 2000 .

[18]  Scott D. Cunningham,et al.  Phytoremediation of Lead-Contaminated Soils: Role of Synthetic Chelates in Lead Phytoextraction , 1997 .

[19]  D. Lestan,et al.  Ethylenediaminedissuccinate as a new chelate for environmentally safe enhanced lead phytoextraction. , 2003, Journal of environmental quality.

[20]  Raskin,et al.  The role of EDTA in lead transport and accumulation by indian mustard , 1998, Plant physiology.

[21]  E. Mückenhausen.  Avery, B.W.: Soil Classification for England and Wales (Higher Categories). Soil Survey, Technical Monograph No. 14, Rothamsted Experimental Station, Harpenden/England 1980. Preis£ 1,— , 1981 .

[22]  Zhenguo Shen,et al.  Lead phytoextraction from contaminated soil with high-biomass plant species. , 2002, Journal of environmental quality.

[23]  Stephen D. Ebbs,et al.  Phytoextraction of Zinc by Oat (Avena sativa), Barley (Hordeum vulgare), and Indian Mustard (Brassica juncea) , 1998 .

[24]  P. W. Jones,et al.  Chemical speciation used to assess [S,S']-ethylenediaminedisuccinic acid (EDDS) as a readily-biodegradable replacement for EDTA in radiochemical decontamination formulations. , 2001, Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine.

[25]  Xiangdong Li,et al.  Leaching and uptake of heavy metals by ten different species of plants during an EDTA-assisted phytoextraction process. , 2004, Chemosphere.

[26]  Lawrence A. Smith,et al.  Remedial options for metals-contaminated sites , 1995 .

[27]  B. W. Avery,et al.  Soil survey laboratory methods , 1974 .

[28]  E. Lombi,et al.  Phytoextraction of heavy metal contaminated soils with Thlaspi goesingense and Amaranthus hybridus: Rhizosphere manipulation using EDTA and ammonium sulfate , 2001 .

[29]  Alan J. M. Baker,et al.  TERRESTRIAL HIGHER PLANTS WHICH HYPERACCUMULATE METALLIC ELEMENTS. A REVIEW OF THEIR DISTRIBUTION, ECOLOGY AND PHYTOCHEMISTRY , 1989 .

[30]  Bernd Nowack,et al.  Environmental chemistry of aminopolycarboxylate chelating agents. , 2002, Environmental science & technology.

[31]  R. Chaney,et al.  Free metal activity and total metal concentrations as indices of micronutrient availability to barley [Hordeum vulgare (L.) ‘Klages’] , 2004, Plant and Soil.

[32]  Zhenguo Shen,et al.  The use of vetiver grass (Vetiveria zizanioides) in the phytoremediation of soils contaminated with heavy metals , 2004 .

[33]  B. Kos,et al.  Induced phytoextraction/soil washing of lead using biodegradable chelate and permeable barriers. , 2003, Environmental science & technology.

[34]  W Verstraete,et al.  Biodegradation of metal-[S,S]-EDDS complexes. , 2001, Environmental science & technology.

[35]  V. Römheld,et al.  Effect of Fe stress on utilization of Fe chelates by efficient and inefficient plant species , 1981 .

[36]  A. Pahlsson,et al.  Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants , 1989 .

[37]  Uri Yermiyahu,et al.  EDTA and Pb—EDTA accumulation in Brassica juncea grown in Pb—amended soil , 1999, Plant and Soil.

[38]  C Garbisu,et al.  Phytoextraction: a cost-effective plant-based technology for the removal of metals from the environment. , 2001, Bioresource technology.

[39]  Battelle Memorial In.  Remedial Options for Metals-Contaminated Sites , 1995 .

[40]  S. McGrath,et al.  Phytoremediation of heavy metal-contaminated soils: natural hyperaccumulation versus chemically enhanced phytoextraction. , 2001, Journal of environmental quality.