Improving biohydrogen production in a carrier-induced granular sludge bed by altering physical configuration and agitation pattern of the bioreactor

Abstract Our newly developed carrier-induced granular sludge bed (CIGSB) bioreactor was shown to be very effective in hydrogen production. However, since mechanical agitation was not employed to enable sludge granulation, the CIGSB system might encounter problems with poor mass transfer efficiency during prolonged operations. This work was undertaken to improve mixing efficiency of CIGSB for better biomass-substrate contact by adjusting the height to diameter (H/D) ratios of the reactor and by implementing appropriate agitation device. Three H/D ratios (4, 8, and 12) resulting in liquid upflow velocities ( v up ) of 0.057–1.32 m/h were examined as the CIGSB reactor was carried out at a descending hydraulic retention time (HRT) from 4 to 0.5 h. The results show that decreasing HRT resulted in increases in the H 2 production rate, regardless of the H/D ratios. Reactors with a H/D ratio of 8 gave better H 2 production performance with a H 2 production rate of 6.87 l/h/l and a H 2 yield of 3.88 mol H 2 /mol sucrose, suggesting that the effectiveness of H 2 production in the CIGSB system can be enhanced by using a proper v up and physical configuration of the reactor. Supply of additional mechanical agitation for CIGSB reactor (H/D=12) alleviated the phenomena of sludge piston floatation, leading to further increases in the H 2 production rate and H 2 yield to 9.31 l/h/l and 4.02 mol H 2 /mol sucrose, respectively. The major soluble metabolite was butyric acid, followed by acetic acid, propionic acid, and ethanol. The former two accounted for nearly 67–76% of total soluble microbial products, indicating the presence of favorable pathways in the CIGSB culture from the aspect of H 2 -producing metabolism.

[1]  G. Lettinga,et al.  Anaerobic digestion and wastewater treatment systems , 2004, Antonie van Leeuwenhoek.

[2]  Gatze Lettinga,et al.  Sustainable integrated biological wastewater treatment , 1996 .

[3]  G Lettinga,et al.  Feasibility of expanded granular sludge bed reactors for the anaerobic treatment of low‐strength soluble wastewaters , 1994, Biotechnology and bioengineering.

[4]  Han-Qing Yu,et al.  Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures , 2002 .

[5]  F. Siñeriz,et al.  High upflow velocity and organic loading rate improve granulation in upflow anaerobic sludge blanket reactors , 1998 .

[6]  Bruno Fabiano,et al.  Process development of continuous hydrogen production by Enterobacter aerogenes in a packed column reactor , 2000 .

[7]  Heguang Zhu,et al.  Hydrogen production from tofu wastewater by Rhodobacter sphaeroides immobilized in agar gels , 1999 .

[8]  Tong Zhang,et al.  Characterization of a hydrogen-producing granular sludge. , 2002, Biotechnology and bioengineering.

[9]  John R. Benemann,et al.  Biological hydrogen production , 1995 .

[10]  Akiko Miya,et al.  Studies on hydrogen production by continuous culture system of hydrogen-producing anaerobic bacteria , 1997 .

[11]  Jo‐Shu Chang,et al.  Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge , 2005 .

[12]  Y. Asada,et al.  Photobiological hydrogen production. , 1999, Journal of bioscience and bioengineering.

[13]  Grietje Zeeman,et al.  A REVIEW: THE ANAEROBIC TREATMENT OF SEWAGE IN UASB AND EGSB REACTORS , 1998 .

[14]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[15]  Jun Hirose,et al.  Hydrogen production by immobilized cells of aciduric Enterobacter aerogenes strain HO-39☆ , 1997 .

[16]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[17]  Patrick C. Hallenbeck,et al.  Biological hydrogen production; fundamentals and limiting processes , 2002 .

[18]  Jo-Shu Chang,et al.  Operation strategies for biohydrogen production with a high-rate anaerobic granular sludge bed bioreactor , 2004 .

[19]  Shigeharu Tanisho,et al.  Continuous hydrogen production from molasses by fermentation using urethane foam as a support of flocks , 1995 .

[20]  Debabrata Das,et al.  Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. , 2001 .

[21]  Chiu-Yue Lin,et al.  Hydrogen production during the anaerobic acidogenic conversion of glucose , 1999 .

[22]  Lawrence Pitt,et al.  Biohydrogen production: prospects and limitations to practical application , 2004 .

[23]  H. Yokoi,et al.  H2 production from starch by a mixed culture of Clostridium butyricum and Enterobacter aerogenes , 1998, Biotechnology Letters.

[24]  Grietje Zeeman,et al.  Advanced anaerobic wastewater treatment in the near future , 1997 .

[25]  Chiu-Yue Lin,et al.  Biohydrogen production using an up-flow anaerobic sludge blanket reactor , 2004 .

[26]  T. Matsunaga,et al.  Microaerobic hydrogen production by photosynthetic bacteria in a double-phase photobioreactor. , 2000, Biotechnology and bioengineering.

[27]  Rolando Chamy,et al.  Comparison of the Behaviour of Expanded Granular Sludge Bed (EGSB) and Upflow Anaerobic Sludge Blanket (UASB) Reactors in Dilute and Concentrated Wastewater Treatment , 1999 .

[28]  Jo-Shu Chang,et al.  Anaerobic hydrogen production with an efficient carrier‐induced granular sludge bed bioreactor , 2004, Biotechnology and bioengineering.

[29]  David F. Ollis,et al.  Biochemical Engineering Fundamentals , 1976 .

[30]  Grietje Zeeman,et al.  Solids removal in upflow anaerobic reactors, a review. , 2003, Bioresource technology.

[31]  G. Lettinga,et al.  Physicochemical and biological performance of expanded granular sludge bed reactors treating long-chain fatty acids , 1998 .

[32]  G. K. Anderson,et al.  The Effect of the Liquid Upflow Velocity and the Substrate Concentration on the Start-Up and Steady-State Periods of Lab-Scale UASB Reactors , 1992 .

[33]  Akashah Majizat,et al.  Hydrogen gas production from glucose and its microbial kinetics in anaerobic systems , 1997 .

[34]  N. Nishio,et al.  Hydrogen production with high yield and high evolution rate by self-flocculated cells of Enterobacter aerogenes in a packed-bed reactor , 1998, Applied Microbiology and Biotechnology.

[35]  D. L. Hawkes,et al.  Sustainable fermentative hydrogen production: challenges for process optimisation , 2002 .

[36]  Jo-Shu Chang,et al.  H2 production with anaerobic sludge using activated-carbon supported packed-bed bioreactors , 2004, Biotechnology Letters.

[37]  Jo-Shu Chang,et al.  Biohydrogen production with fixed-bed bioreactors , 2002 .

[38]  J. Lay,et al.  Modeling and optimization of anaerobic digested sludge converting starch to hydrogen , 2000, Biotechnology and bioengineering.

[39]  Chin-Chao Chen,et al.  Acid–base enrichment enhances anaerobic hydrogen production process , 2001, Applied Microbiology and Biotechnology.

[40]  Serge R. Guiot,et al.  Advantages of Fluidization on Granule Size and Activity Development in Upflow Anaerobic Sludge Bed Reactors , 1992 .