论文信息 - Short-term, high-efficiency expression of transfected DNA

Short-term, high-efficiency expression of transfected DNA

We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.

D. Sussman | G. Milman

[1] I. Pastan,et al. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[2] K. Danna,et al. Efficient infection of monkey cells with DNA of simian virus 40. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[3] L. Siminovitch,et al. Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA , 1980, Somatic Cell Genetics.

[4] A. van der Eb,et al. A new technique for the assay of infectivity of human adenovirus 5 DNA. , 1973, Virology.