Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode

Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 Ω cm2 and sustains a "resting" potential, the nucleoplasm being about 15 mv negative with respect to the cytoplasm. Both the resistance and potential appear to be associated with the nuclear membrane: the potential declines to zero and the resistance to a fraction of its original value, when the membrane is perforated experimentally.

[1]  S. Wischnitzer.  An electron microscope study of the nuclear envelope of amphibian oocytes. , 1958, Journal of ultrastructure research.

[2]  R. Merriam.  ON THE FINE STRUCTURE AND COMPOSITION OF THE NUCLEAR ENVELOPE , 1961, The Journal of biophysical and biochemical cytology.

[3]  R. H. Adrian,et al.  The effect of internal and external potassium concentration on the membrane potential of frog muscle , 1956, The Journal of physiology.

[4]  Kenneth S. Cole,et al.  PERMEABILITY AND IMPERMEABILITY OF CELL MEMBRANES FOR IONS , 1940 .

[5]  J. Gall.  Observations on the nuclear membrane with the electron microscope. , 1954, Experimental cell research.

[6]  M. L. Watson.  Further Observations on the Nuclear Envelope of the Animal Cell , 1959, The Journal of biophysical and biochemical cytology.

[7]  W. Loewenstein,et al.  THE ELECTRICAL CONDUCTANCE AND POTENTIAL ACROSS THE MEMBRANE OF SOME CELL NUCLEI , 1963, The Journal of cell biology.

[8]  K. Cole.  Some physical aspects of bioelectric phenomena. , 1949, Proceedings of the National Academy of Sciences of the United States of America.

[9]  G. Pappas.  THE FINE STRUCTURE OF THE NUCLEAR ENVELOPE OF AMOEBA PROTEUS , 1956, The Journal of biophysical and biochemical cytology.

[10]  W. Loewenstein,et al.  Some Electrical Properties of the Membrane of a Cell Nucleus , 1962, Nature.

[11]  W. Loewenstein,et al.  A STUDY OF THE NUCLEUS AND CELL MEMBRANES OF OOCYTES WITH AN INTRA-CELLULAR ELECTRODE. , 1963, Experimental cell research.

[12]  S. Palay,et al.  THE FINE STRUCTURE OF NEURONS , 1955, The Journal of biophysical and biochemical cytology.

[13]  H. G. Callan,et al.  Experimental studies on amphibian oocyte nuclei I. Investigation of the structure of the nuclear membrane by means of the electron microscope , 1950, Proceedings of the Royal Society of London. Series B - Biological Sciences.

[14]  G. Bahr,et al.  The fine structure of the nuclear membrane in the larval salivary gland and midgut of Chironomus. , 1954, Experimental cell research.

[15]  A. Hodgkin,et al.  The electrical constants of a crustacean nerve fibre , 1946, Proceedings of the Royal Society of London. Series B - Biological Sciences.

[16]  A. Lundberg,et al.  The electrophysiology of the submaxillary gland of the cat. , 1955, Acta physiologica Scandinavica.

[17]  A. Mirsky,et al.  The Interphase Nucleus , 1961 .

[18]  R W GERARD,et al.  The normal membrane potential of frog sartorius fibers. , 1949, Journal of cellular and comparative physiology.

[19]  H. Gay.  CHROMOSOME-NUCLEAR MEMBRANE-CYTOPLASMIC INTERRELATIONS IN DROSOPHILA , 1956, The Journal of biophysical and biochemical cytology.

[20]  M. L. Watson.  THE NUCLEAR ENVELOPE: ITS STRUCTURE AND RELATION TO CYTOPLASMIC MEMBRANES , 1955 .

[21]  B. Katz,et al.  An analysis of the end‐plate potential recorded with an intra‐cellular electrode , 1951, The Journal of physiology.

[22]  A. Mirsky,et al.  Sodium-dependent "transport" reactions in the cell nucleus and their role in protein and nucleic acid synthesis. , 1961, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. D. Robertson,et al.  The ultrastructure of cell membranes and their derivatives. , 1959, Biochemical Society symposium.

[24]  M. Kopac,et al.  MALIGNANT NUCLEOLI: CYTOLOGICAL STUDIES AND PERSPECTIVES , 1958, Annals of the New York Academy of Sciences.

[25]  B. Afzelius.  The ultrastructure of the nuclear membrane of the sea urchin oocyte as studied with the electron microscope. , 1955, Experimental cell research.