Tunable narrow band filter for CARS microscopy

In this letter we present an approach to CARS microscopy, which compromises between fast acquisition rates and the amount of chemical information obtained. By using a light modulator as tunable filter in concert with narrowband pump and broadband Stokes pulses, we demonstrate an experimental arrangement, which allows for fast electronic switching between CARS images recorded at different Raman resonances without the need for any optical adjustment.

[1]  David Y. Smith.  Dispersion relations for complex reflectivities , 1977 .

[2]  Yaochun Shen.  Principles of nonlinear optics , 1984 .

[3]  Hiro-o Hamaguchi,et al.  Broadband (1000 cm−1) multiplex CARS spectroscopy: Application to polarization sensitive and time-resolved measurements , 1994 .

[4]  Keith A. Nelson,et al.  Analysis of programmable ultrashort waveform generation using liquid-crystal spatial light modulators , 1995 .

[5]  George Turrell,et al.  Raman microscopy : developments and applications , 1996 .

[6]  Pierre Tournois,et al.  Acousto-optic programmable dispersive filter for adaptive compensation of group delay time dispersion in laser systems , 1997 .

[7]  V Laude,et al.  Amplitude and phase control of ultrashort pulses by use of an acousto-optic programmable dispersive filter: pulse compression and shaping. , 2000, Optics letters.

[8]  G. Gerber,et al.  Photoselective adaptive femtosecond quantum control in the liquid phase , 2001, Nature.

[9]  Jürgen Popp,et al.  Confocal Raman investigations on hybrid polymer coatings , 2002 .

[10]  Marcus Motzkus,et al.  Quantum control of energy flow in light harvesting , 2002, Nature.

[11]  Jürgen Popp,et al.  Raman spectroscopy--a prospective tool in the life sciences. , 2003, Chemphyschem : a European journal of chemical physics and physical chemistry.

[12]  Yaron Silberberg,et al.  Femtosecond phase-and-polarization control for background-free coherent anti-Stokes Raman spectroscopy. , 2003, Physical review letters.

[13]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[14]  M. A. Strehle,et al.  Chemotaxonomic characterisation of essential oil plants by vibrational spectroscopy measurements , 2004 .

[15]  All-optical processing in coherent nonlinear spectroscopy , 2004 .

[16]  Andreas Volkmer,et al.  Vibrational imaging and microspectroscopies based on coherent anti-Stokes Raman scattering microscopy , 2005 .

[17]  H. A. Rinia,et al.  Imaging orientational order and lipid density in multilamellar vesicles with multiplex CARS microscopy , 2005, Journal of microscopy.

[18]  H. Hamaguchi,et al.  In-vivo multi-nonlinear optical imaging of a living cell using a supercontinuum light source generated from a photonic crystal fiber. , 2006, Optics express.

[19]  Jürgen Popp,et al.  Raman spectroscopy at the beginning of the twenty-first century , 2006 .

[20]  Marcus Motzkus,et al.  Actively shaped supercontinuum from a photonic crystal fiber for nonlinear coherent microspectroscopy. , 2006, Optics letters.

[21]  Annika Enejder,et al.  Dual-pump coherent anti-Stokes-Raman scattering microscopy. , 2006, Optics letters.

[22]  Alexander Marx,et al.  Immuno‐Raman microspectroscopy: In situ detection of antigens in tissue specimens by surface‐enhanced Raman scattering , 2006 .

[23]  Jürgen Popp,et al.  On-line monitoring and identification of bioaerosols. , 2006, Analytical chemistry.

[24]  Benjamin Dietzek,et al.  Mechanisms of molecular response in the optimal control of photoisomerization. , 2006, Physical review letters.

[25]  Mischa Bonn,et al.  Quantitative CARS spectroscopy using the maximum entropy method: the main lipid phase transition. , 2007, Chemphyschem : a European journal of chemical physics and physical chemistry.

[26]  Benjamin Dietzek,et al.  Pump-shaped dump optimal control reveals the nuclear reaction pathway of isomerization of a photoexcited cyanine dye. , 2007, Journal of the American Chemical Society.

[27]  Marcus Motzkus,et al.  Molecular discrimination of a mixture with single-beam Raman control. , 2007, The Journal of chemical physics.

[28]  Benjamin Dietzek,et al.  Tracking ultrafast excited-state bond-twisting motion in solution close to the Franck-Condon point. , 2007, The journal of physical chemistry. B.

[29]  Andreas Zumbusch,et al.  Coherent anti-Stokes Raman scattering microscopy , 1999 .

[30]  I. M. Vlasova,et al.  Raman spectroscopy in investigations of mechanism of binding of human serum albumin to molecular probe fluorescein , 2008 .

[31]  Beate Bornschein,et al.  Dynamic Raman spectroscopy of hydrogen isotopomer mixtures in-line at TILO , 2008 .

[32]  Jürgen Popp,et al.  Three-dimensional molecular mapping of a multiple emulsion by means of CARS microscopy. , 2008, The journal of physical chemistry. B.

[33]  Ota Samek,et al.  Raman spectroscopy for rapid discrimination of Staphylococcus epidermidis clones related to medical device-associated infections , 2008 .

[34]  J P Korterik,et al.  Background free CARS imaging by phase sensitive heterodyne CARS. , 2008, Optics express.

[35]  Max Diem,et al.  New ways of imaging uptake and intracellular fate of liposomal drug carrier systems inside individual cells, based on Raman microscopy. , 2008, Molecular pharmaceutics.

[36]  Jürgen Popp,et al.  Tunable light source for narrowband laser excitation: application to Raman spectroscopy , 2009 .

[37]  Jürgen Popp,et al.  Quantitative CARS microscopic detection of analytes and their isotopomers in a two-channel microfluidic chip. , 2009, Small.

[38]  Maxim E. Darvin,et al.  Influence of two different IR radiators on the antioxidative potential of the human skin , 2009 .

[39]  Wei Min,et al.  Triple-resonance coherent anti-stokes Raman scattering microspectroscopy. , 2009, Chemphyschem : a European journal of chemical physics and physical chemistry.

[40]  B. Dietzek,et al.  Raman and CARS microspectroscopy of cells and tissues. , 2009, The Analyst.

[41]  Jürgen Popp,et al.  Different contrast information obtained from CARS and nonresonant FWM images , 2009 .

[42]  Jürgen Popp,et al.  A comparative Raman and CARS imaging study of colon tissue , 2009, Journal of biophotonics.

[43]  Maxim E. Darvin,et al.  In vivo Raman spectroscopy detects increased epidermal antioxidative potential with topically applied carotenoids , 2008 .

[44]  B. Dietzek,et al.  Tunable optical setup with high flexibility for spectrally resolved coherent anti-Stokes Raman scattering microscopy , 2011 .