A novel approach to prepare extended DNA fibers in plants

The extended DNA fiber preparation procedure is still imperfect in plants due to the existence of a hard cell wall; thus, high quality of extended DNA fibers for fluorescence in situ hybridization (FISH) analysis is often difficult to be obtained rapidly and efficiently. In this study we have developed a fast and widely effective method to prepare DNA fibers from various plant species and the fibers are suitable for fiber FISH mapping.

[1]  X. Zhong,et al.  High-resolution mapping on pachytene chromosomes and extended DNA fibres by fluorescencein-situ hybridisation , 1996, Plant Molecular Biology Reporter.

[2]  E. Earle,et al.  Estimation of nuclear DNA content of plants by flow cytometry , 1991, Plant Molecular Biology Reporter.

[3]  Steven Henikoff,et al.  Maize Centromeres: Organization and Functional Adaptation in the Genetic Background of Oat , 2004, The Plant Cell Online.

[4]  G. Martin,et al.  Chromosome 2-specific DNA clones from flow-sorted chromosomes of tomato , 1994, Molecular and General Genetics MGG.

[5]  A. Aurias,et al.  Color bar coding the BRCA1 gene on combed DNA: A useful strategy for detecting large gene rearrangements , 2001, Genes, chromosomes & cancer.

[6]  D. Sandhu,et al.  Flow cytometric sorting of maize chromosome 9 from an oat-maize chromosome addition line , 2001, Theoretical and Applied Genetics.

[7]  H. Goodman,et al.  Comparative fluorescence in situ hybridization mapping of a 431-kb Arabidopsis thaliana bacterial artificial chromosome contig reveals the role of chromosomal duplications in the expansion of the Brassica rapa genome. , 2000, Genetics.

[8]  K. Fukui,et al.  Quantification of total genomic DNA and selected repetitive sequences reveals concurrent changes in different DNA families in indica and japonica rice , 2000, Molecular and General Genetics MGG.

[9]  C. Dean,et al.  Integrated Cytogenetic Map of Chromosome Arm 4S of A. thaliana Structural Organization of Heterochromatic Knob and Centromere Region , 2000, Cell.

[10]  C. Coutelle,et al.  Analysis of ribosomal and alphoid repetitive DNA by fiber-FISH. , 1997, Cytogenetics and cell genetics.

[11]  A. Peeters,et al.  High-resolution physical mapping in Arabidopsis thaliana and tomato by fluorescence in situ hybridization to extended DNA fibres. , 1996, The Plant journal : for cell and molecular biology.

[12]  A. Palotie,et al.  Head to tail organization of the human COL6A1 and COL6A2 genes by fiber-FISH. , 1995, Genomics.

[13]  J. Mullikin,et al.  Quantitative DNA fiber mapping. , 1995, Human molecular genetics.

[14]  Y. G. Liu,et al.  Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads. , 1994, Nucleic acids research.

[15]  B. Windle,et al.  High resolution visual mapping of stretched DNA by fluorescent hybridization , 1993, Nature Genetics.

[16]  D. Galbraith,et al.  Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues , 1983, Science.