Differences in adrenergic recognition by pancreatic A and B cells.

The adrenergic control of glucose homeostasis is mediated in part through variations in the release of pancreatic hormones. In this study, purified pancreatic A and B cells were used to identify the recognition and messenger units involved in the adrenergic regulation of glucagon and insulin release. Catecholamines induced beta-adrenergic receptor activity in A cells and alpha 2-adrenergic receptor activity in B cells. The two recognition units provoked opposite variations in the production of cellular cyclic adenosine monophosphate, the beta-adrenergic unit enhancing the nucleotide's permissive effect on amino acid-induced glucagon release and the alpha 2-adrenergic unit inhibiting that upon glucose-induced insulin release. In both cell types, catecholamines interact powerfully with the synergistic control of hormone release by nutrient- and (neuro)hormone-driven messenger systems.

[1]  D. Pipeleers,et al.  Regulation of adenosine 3',5'-monophosphate levels in the pancreatic B cell. , 1985, Endocrinology.

[2]  V. Rogiers,et al.  Pancreatic hormone receptors on islet cells. , 1985, Endocrinology.

[3]  E. Hooghe-Peters,et al.  Interplay of nutrients and hormones in the regulation of insulin release. , 1985, Endocrinology.

[4]  D. Pipeleers,et al.  A new in vitro model for the study of pancreatic A and B cells. , 1985, Endocrinology.

[5]  C. Wollheim,et al.  Islet cyclic AMP levels are not lowered during alpha 2-adrenergic inhibition of insulin release. , 1984, The Journal of biological chemistry.

[6]  J. Holly,et al.  The estimation of catecholamines in human plasma. , 1983, Analytical biochemistry.

[7]  D Pipeleers,et al.  Glucose-induced insulin release depends on functional cooperation between islet cells. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[8]  D. Pipeleers,et al.  Islet cell analysis and purification by light scatter and autofluorescence. , 1982, Biochemical and biophysical research communications.

[9]  L. Orci,et al.  Glucagon and the A cell: physiology and pathophysiology (second of two parts). , 1981, The New England journal of medicine.

[10]  T. Nakaki,et al.  Postsynaptic alpha-2 adrenergic receptors in isolated rat islets of Langerhans: inhibition of insulin release and cyclic 3':5'-adenosine monophosphate accumulation. , 1981, The Journal of pharmacology and experimental therapeutics.

[11]  P. Cryer Physiology and pathophysiology of the human sympathoadrenal neuroendocrine system. , 1980, The New England journal of medicine.

[12]  R. Elde,et al.  Effect of Norepinephrine on Insulin, Glucagon, and Somatostatin Secretion in Isolated Perifused Rat Islets , 1979, Diabetes.

[13]  G. Weir,et al.  Adrenergic modulation of pancreatic A, B, and D cells alpha-Adrenergic suppression and beta-adrenergic stimulation of somatostatin secretion, alpha-adrenergic stimulation of glucagon secretion in the perfused dog pancreas. , 1979, The Journal of clinical investigation.

[14]  E. Cerasi,et al.  Adenosine 3',5'-monophosphate-dependent and -independent inhibitory effects of epinephrine on insulin release in rat pancreatic islets. , 1978, Endocrinology.

[15]  R. Williams,et al.  Inhibition of Insulin Release by Scorpion Toxin in Rat Pancreatic Islets , 1976, Diabetes.

[16]  J. Gerich,et al.  Adrenergic modulation of pancreatic glucagon secretion in man. , 1974, The Journal of clinical investigation.

[17]  J. Iversen Adrenergic receptors and the secretion of glucagon and insulin from the isolated, perfused canine pancreas. , 1973, The Journal of clinical investigation.

[18]  V. J. Maglio Vertebrate Faunas from the Kubi Algi, Koobi Fora and Ileret Areas, East Rudolf, Kenya , 1971, Nature.

[19]  D. Kipnis,et al.  An adrenergic receptor mechanism for the control of cyclic 3'5' adenosine monophosphate synthesis in tissues. , 1967, Biochemical and biophysical research communications.

[20]  D. Porte,et al.  Inhibition of Insulin Release by Norepinephrine in Man , 1966, Science.

[21]  P. J. Randle,et al.  Regulation of insulin secretion studied with pieces of rabbit pancreas incubated in vitro. , 1964, The Biochemical journal.