Métabolisme sucre-citrate chez Leuconostoc mesenteroides

Sugar citrate cometabolism in Leuconostoc mesenteroides. Bacteria from the genus Leuconostocplay roles in the dairy industry. The most important functions of this bacteria are their ability to produce CO 2 and flavour compounds through lactose heterofermentation and citrate uti- lization. Although the biotechnological role of the citrate metabolism is very important and widely appreciated, little is known about the genetic properties of Leuconostoc spp. In our laboratory, we cloned the genes responsible for citrate metabolism ( clyR mae citCDEFGOPcluster), for D-lactate dehydrogenase (ldhD) and for phosphotransacetylase ( pta). In addition we have planned to con- struct new vectors and we have tried to improve a method to introduce recombinant DNA molecules into Leuconostoc as well. Characterization of the plasmid involved in this study is still in progress. The nucleotide sequence analysis of p22R revealed the presence of C5 cytosine methylase gene typ- ical of type II restriction/modification system. The low transformation efficiency of some strains of Leuconostoc may be due to the presence of that plasmid. The construction of the defective strain for restriction activity could be very useful to genetic manipulations of Leuconostocin the future. metabolic engineering / lactic acid bacteria / Leuconostoc mesenteroides / citrate / plasmid / restriction modification

[1]  C. Diviès,et al.  Genetic Organization of the citCDEFLocus and Identification of mae and clyR Genes from Leuconostoc mesenteroides , 1999, Journal of bacteriology.

[2]  W. Konings,et al.  Mechanism of the Citrate Transporters in Carbohydrate and Citrate Cometabolism in Lactococcus andLeuconostoc Species , 1998, Applied and Environmental Microbiology.

[3]  J. V. Van Beeumen,et al.  Purification of Leuconostoc mesenteroides Citrate Lyase and Cloning and Characterization of the citCDEFGGene Cluster , 1998, Journal of bacteriology.

[4]  C. Diviès,et al.  Citrate lyases of lactic acid bacteria , 1998 .

[5]  F. Vogensen,et al.  Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids. , 1997, Plasmid.

[6]  S. Bekal,et al.  Citrate permease gene expression in Lactococcus lactis subsp. lactis strains IL1403 and MG1363 , 1996 .

[7]  A. Schanck,et al.  13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum , 1996, Journal of bacteriology.

[8]  J. Richardson,et al.  Knockout of the two ldh genes has a major impact on peptidoglycan precursor synthesis in Lactobacillus plantarum , 1996, Journal of bacteriology.

[9]  C. Diviès,et al.  Instability of plasmid‐encoded citrate permease in Leuconostoc , 1996 .

[10]  D. J. Leblanc,et al.  Use of a novel mobilizable vector to inactivate the scrA gene of Streptococcus sobrinus by allelic replacement , 1995, Journal of bacteriology.

[11]  F. Vogensen,et al.  Characterization of the replicon from the lactococcal theta-replicating plasmid pJW563. , 1995, Plasmid.

[12]  F. Vogensen,et al.  Restriction-modification systems in Lactococcus lactis. , 1995, Gene.

[13]  V. Dartois,et al.  Purification, properties and DNA sequence of the D-lactate dehydrogenase from Leuconostoc mesenteroides subsp. cremoris. , 1995, Research in microbiology.

[14]  D. Garmyn,et al.  Use of homologous expression-secretion signals and vector-free stable chromosomal integration in engineering of Lactobacillus plantarum for alpha-amylase and levanase expression , 1994, Applied and environmental microbiology.

[15]  D. Garmyn,et al.  Lactobacillus plantarum ldhL gene: overexpression and deletion , 1994, Journal of bacteriology.

[16]  Jinping,et al.  Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate , 1994, Journal of bacteriology.

[17]  C. Diviès,et al.  Instability of plasmid encoding for β‐galactosidase in Leuconostoc mesenteroides subsp. mesenteroides , 1994 .

[18]  J. Hugenholtz.  Citrate metabolism in lactic acid bacteria , 1993 .

[19]  A. Ninfa,et al.  Is acetyl phosphate a global signal in Escherichia coli? , 1993, Journal of bacteriology.

[20]  E. Maguin,et al.  New thermosensitive plasmid for gram-positive bacteria , 1992, Journal of bacteriology.

[21]  T. Cogan.  Co‐metabolism of citrate and glucose by Leuconostoc spp.: effects on growth, substrates and products , 1987 .

[22]  S. Condon,et al.  Active Role of Oxygen and NADH Oxidase in Growth and Energy Metabolism of Leuconostoc , 1986 .

[23]  W. Sandine,et al.  Common occurrence of plasmid DNA and vancomycin resistance in Leuconostoc spp , 1984, Applied and environmental microbiology.

[24]  T. Cogan,et al.  Induction of some enzymes of citrate metabolism in Leuconostoc lactis and other heterofermentative lactic acid bacteria , 1981, Journal of Dairy Research.