Isometric force and endurance in soleus muscle of thyroid hormone receptor-α1- or -β-deficient mice

The specific role of each subtype of thyroid hormone receptor (TR) on skeletal muscle function is unclear. We have therefore studied kinetics of isometric twitches and tetani as well as fatigue resistance in isolated soleus muscles of R-α1- or -β-deficient mice. The results show 20–40% longer contraction and relaxation times of twitches and tetani in soleus muscles from TR-α1-deficient mice compared with their wild-type controls. TR-β-deficient mice, which have high thyroid hormone levels, were less fatigue resistant than their wild-type controls, but contraction and relaxation times were not different. Western blot analyses showed a reduced concentration of the fast-type sarcoplasmic reticulum Ca2+-ATPase (SERCa1) in TR-α1-deficient mice, but no changes were observed in TR-β-deficient mice compared with their respective controls. We conclude that in skeletal muscle, both TR-α1 and TR-β are required to get a normal thyroid hormone response.

[1]  F. Haddad,et al.  Thyroid receptor plasticity in striated muscle types: effects of altered thyroid state. , 1998, American journal of physiology. Endocrinology and metabolism.

[2]  A. Harrison,et al.  Thyroid hormone-induced upregulation of Na+ channels and Na+-K+pumps: implications for contractility. , 1998, American journal of physiology. Regulatory, integrative and comparative physiology.

[3]  Frank Baas,et al.  Abnormal heart rate and body temperature in mice lacking thyroid hormone receptor α1 , 1998, The EMBO journal.

[4]  H. Westerblad,et al.  Increased tetanic force and reduced myoplasmic [P(i)] following a brief series of tetani in mouse soleus muscle. , 1997, The American journal of physiology.

[5]  D. Allen,et al.  Slowed Relaxation in Fatigued Skeletal Muscle Fibers of Xenopus and Mouse , 1997, The Journal of general physiology.

[6]  K. Nair,et al.  Hormonal regulation of human muscle protein metabolism. , 1997, Annual review of nutrition.

[7]  L. Larsson,et al.  Thyroid hormone effects on contractility and myosin composition of soleus muscle and single fibres from young and old rats. , 1996, The Journal of physiology.

[8]  D. Forrest,et al.  Recessive resistance to thyroid hormone in mice lacking thyroid hormone receptor beta: evidence for tissue‐specific modulation of receptor function. , 1996, The EMBO journal.

[9]  M. Everts.  Effects of thyroid hormones on contractility and cation transport in skeletal muscle. , 1996, Acta physiologica Scandinavica.

[10]  L. Larsson,et al.  Effects of thyroid hormone on fast‐ and slow‐twitch skeletal muscles in young and old rats. , 1994, The Journal of physiology.

[11]  C. van Hardeveld,et al.  Differential effects of thyroid hormone on the expression of sarcoplasmic reticulum Ca(2+)-ATPase isoforms in rat skeletal muscle fibers. , 1994, Biochemical and biophysical research communications.

[12]  S. Swoap,et al.  Single fiber analyses of type IIA myosin heavy chain distribution in hyper- and hypothyroid soleus. , 1993, The American journal of physiology.

[13]  P. Hespel,et al.  Glucose transport and transporters in muscle giant vesicles: differential effects of insulin and contractions. , 1993, The American journal of physiology.

[14]  M. R. Sayen,et al.  Thyroid hormone response of slow and fast sarcoplasmic reticulum Ca2+ ATPase mRNA in striated muscle , 1992, Molecular and Cellular Endocrinology.

[15]  N. Alpert,et al.  Effect of thyroid hormone on the expression of mRNA encoding sarcoplasmic reticulum proteins. , 1991, Circulation research.

[16]  D. Forrest,et al.  Distinct functions for thyroid hormone receptors alpha and beta in brain development indicated by differential expression of receptor genes. , 1991, The EMBO journal.

[17]  C. Glass,et al.  Regulation of gene expression by the thyroid hormone receptor. , 1990, Biochimica et biophysica acta.

[18]  C. van Hardeveld,et al.  Thyroid hormone differentially affects mRNA levels of Ca‐ATPase isozymes of sarcoplasmic reticulum in fast and slow skeletal muscle , 1990, FEBS letters.

[19]  D. Forrest,et al.  Contrasting developmental and tissue‐specific expression of alpha and beta thyroid hormone receptor genes. , 1990, The EMBO journal.

[20]  J. Lingrel,et al.  Tissue distribution of mRNAs encoding the α isoforms and β subunit of rat Na+,K+-ATPase , 1987 .

[21]  P. Hasselgren,et al.  Protein turnover in different types of skeletal muscle during experimental hyperthyroidism in rats. , 1985, Acta endocrinologica.

[22]  R. Fitts,et al.  Contractile, biochemical, and histochemical properties of thyrotoxic rat soleus muscle. , 1980, The American journal of physiology.

[23]  T. Clausen,et al.  Thyroid hormones and the energetics of active sodium‐potassium transport in mammalian skeletal muscles. , 1979, The Journal of physiology.

[24]  D. Jones,et al.  Muscle relaxation rate, fibre-type composition and energy turnover in hyper- and hypo-thyroid patients. , 1979, Clinical science.

[25]  J. Oppenheimer.  Thyroid hormone action at the cellular level. , 1979, Science.

[26]  B. Fanburg,et al.  Calcium Transport in Isolated Sarcoplasmic Reticulum during Muscle Maturation , 1968, Nature.