Transitory mobile phase environments for rapid selectivity changes in liquid chromatography: application to organic dyestuffs

Transient mobile phase environments created by injecting solvent plugs of varying composition are shown to selectively elute compounds according to their chemical properties. With several organic dyestuffs as model solutes, weakly acidic, strongly acidic, and neutral substances were sequentially eluted in a series of subchromatograms based on pH, ion-pair competition, and hydrophobic interaction mechanisms, respectively. Elution with both isocratic plugs and with plug-generated gradients is described. The use of transparent columns allowed solute bands to be viewed during the separation process, and the potential for on-column detection and laser scanning is discussed.

[1]  C. Enke,et al.  Whole column detection chromatography: computer simulations , 1986 .

[2]  K. Sˇlais,et al.  Trace analysis by injection-generated gradient ion-pair chromatography with micro-bore column high-performance liquid chromatography , 1986 .

[3]  S. Folestad,et al.  Polymerized stationary phases in 12 to 50 μm open tubular fused silica columns for LC and GC , 1986 .

[4]  J. Jorgenson,et al.  Scanning on-column voltammetric detector for open-tubular liquid chromatography. , 1986, Analytical chemistry.

[5]  Norman J. Dovichi,et al.  Sub-picoliter detection with the sheath flow cuvette , 1985 .

[6]  H. Billiet,et al.  Effect of the type of ion-pairing reagent in reversed-phase ion-pair chromatography , 1985 .

[7]  M. P. Maskarinec,et al.  Dynamically modified capillary columns for liquid chromatographic separations of biological compounds. , 1985, Journal of chromatographic science.

[8]  D. Westerlund,et al.  Peak compression effects in ion-pair reversed-phase liquid chromatography of substituted benzamides , 1985 .

[9]  W. Niessen,et al.  Studies on external peak broadening in open-tubular liquid chromatography systems using the exponentially modified Gaussian model , 1985 .

[10]  J. Jorgenson,et al.  Characterization of an on-column electrochemical detector for open-tubular liquid chromatography , 1985 .

[11]  W. Niessen,et al.  Open-tubular liquid chromatography—mass spectrometry using direct liquid introduction , 1985 .

[12]  H. Poppe,et al.  The performance of some cell designs for laser-induced fluorescence detection in open-tubular liquid chromatography , 1985 .

[13]  V. Berry.  Universal Liquid Chromatography Methods : V. “Pulsing” with weak eluent to resolve peaks☆ , 1985 .

[14]  J. E. Bailey.  Determination of the lower sulfonated subsidiary colors in FD&C Yellow No. 6 by reversed-phase high-performance liquid chromatography. , 1985, Journal of chromatography.

[15]  M. Novotny,et al.  Dead-volume free termination for packed columns in microcapillary liquid chromatography. , 1984, Analytical chemistry.

[16]  J. Foley,et al.  Clarification of the limit of detection in chromatography , 1984 .

[17]  V. Berry.  Universal liquid chromatography methods : IV. Use of 190-mm full gradients by adjustment of eluent ultraviolet absorbance and pH with gases , 1984 .

[18]  J. Jorgenson,et al.  On-Column Fluorescence Detector for Open-Tubular Capillary Liquid Chromatography , 1984 .

[19]  H. Billiet,et al.  Studies in reversed-phase ion-pair chromatography : IV. The rôle of the chain length of the pairing ion , 1984 .

[20]  Vern V. Berry,et al.  Universal liquid chromatographic methods : III. Sensitive, low-wavelength gradients with a novel injection-loading ion-pairing technique☆ , 1984 .

[21]  Joe M. Davis,et al.  Statistical theory of component overlap in multicomponent chromatograms , 1983 .

[22]  K. Šlais,et al.  Electrochemical cell with effective volume less than 1 nl for liquid chromatography , 1982 .

[23]  W. Simon,et al.  Neutral carrier based ion-selective electrode for intracellular magnesium activity studies , 1980 .

[24]  P. Kucera.  Design and use of short microbore columns in liquid chromatography , 1980 .

[25]  P. Jandera,et al.  Reversed-phase liquid chromatography of aromatic sulfonic and carboxylic acids using inorganic electrolyte solutions as the mobile phase , 1980 .

[26]  P. Jandera,et al.  Liquid chromatography separation of organic acidic compounds , 1980 .

[27]  H. Engelhardt.  High performance liquid chromatography , 1997 .

[28]  S. Deming,et al.  Retention mechamism for reversed-phase ion-pair liquid chromatography , 1979 .

[29]  R. S. Deelder,et al.  Retention behaviour of carboxylic acids in reversed-phase column liquid chromatography , 1978 .

[30]  J. Crommen,et al.  Ion-pair chromatography in the low concentration range by use of highly absorbing counter ions , 1977 .

[31]  R. Gloor,et al.  Practical Aspects off Reverse Phase Ion Pair Chromatography , 1977 .

[32]  J. Knox,et al.  Soap chromatography—a new high-performance liquid chromatographic technique for separation of ionizable materials , 1976 .

[33]  W. G. Haney,et al.  Simultaneous analysis of tartrazine and its intermediates by reversed phase liquid chromatography. , 1975, Analytical chemistry.

[34]  H. Engelhardt,et al.  Influence of the water content of the solvent on chromatographic separations , 1974 .