Calcium mobilization and exocytosis after one mechanical stretch of lung epithelial cells.

Deep inflation of the lung stimulates surfactant secretion by unknown mechanisms. The hypothesis that mechanical distension directly stimulates type II cells to secrete surfactant was tested by stretching type II cells cultured on silastic membranes. The intracellular Ca2+ concentration was measured in single cells, before and after stretching. A single stretch of alveolar type II cells caused a transient (less than 60 seconds) increase in cytosolic Ca2+ followed by a sustained (15 to 30 minutes) stimulation of surfactant secretion. Both Ca2+ mobilization and exocytosis exhibited dose-dependence to the magnitude of the stretch-stimulus. Thus, mechanical factors can trigger complex cellular events in nonneuron, nonmuscle cells and may be involved in regulating normal lung functions.

[1]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[2]  J. Power,et al.  Surfactant homeostasis in the rat lung during swimming exercise. , 1982, Journal of applied physiology: respiratory, environmental and exercise physiology.

[3]  J. Wright,et al.  Pulmonary surfactant and its components inhibit secretion of phosphatidylcholine from cultured rat alveolar type II cells. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Whitsett,et al.  Surfactant-associated protein inhibits phospholipid secretion from type II cells. , 1987, Journal of applied physiology.

[5]  T. Nicholas,et al.  Control of release of surfactant phospholipids in the isolated perfused rat lung. , 1981, Journal of applied physiology: respiratory, environmental and exercise physiology.

[6]  N. Düzgüneş,et al.  Positive correlation between cytosolic free calcium and surfactant secretion in cultured rat alveolar type II cells. , 1988, Biochimica et biophysica acta.

[7]  E. Weibel,et al.  Alveolar volume-surface area relation in air- and saline-filled lungs fixed by vascular perfusion. , 1979, Journal of applied physiology: respiratory, environmental and exercise physiology.

[8]  P. Watson,et al.  Accumulation of cAMP and calcium in S49 mouse lymphoma cells following hyposmotic swelling. , 1989, The Journal of biological chemistry.

[9]  J. Goerke,et al.  Surfactant release in excised rat lung is stimulated by air inflation. , 1981, Journal of applied physiology: respiratory, environmental and exercise physiology.

[10]  T. Rink,et al.  Single stretch-activated ion channels in vascular endothelial cells as mechanotransducers? , 1987, Nature.

[11]  T. Nicholas,et al.  The release of surfactant in rat lung by brief periods of hyperventilation. , 1983, Respiration physiology.

[12]  J. Power,et al.  The pulmonary consequences of a deep breath. , 1982, Respiration physiology.

[13]  S. Hawgood,et al.  The role of calcium in the secretion of surfactant by rat alveolar type II cells. , 1986, Biochimica et biophysica acta.

[14]  R. Riley,et al.  Effect of ventilation on surface forces in excised dogs' lungs. , 1966, Journal of applied physiology.

[15]  L. Brown,et al.  Adrenergic and cholinergic regulation of lung surfactant secretion in the isolated perfused rat lung and in the alveolar type II cell in culture. , 1981, The Journal of biological chemistry.

[16]  K. Kyei-Aboagye,et al.  Surfactant inactivation by hyperventilation: conservation by end-expiratory pressure. , 1975, Journal of applied physiology.

[17]  J. Edmonds,et al.  Release of prostaglandin E2 and unidentified factors from ventilated lungs , 1971, The British journal of surgery.