Riboswitches exert genetic control through metabolite-induced conformational change.

Conserved RNA structures have traditionally been thought of as potential binding sites for protein factors and consequently are regarded as fulfilling relatively passive albeit important roles in cellular processes. With the discovery of riboswitches, RNA no longer takes a backseat to protein when it comes to affecting gene expression. Riboswitches bind directly to cellular metabolites with exceptional specificity and affinity, and exert control over gene expression through ligand-induced conformational changes in RNA structure. Riboswitches now represent a widespread mechanism by which cells monitor their metabolic state and facilely alter gene expression in response to changing conditions.

[1]  E. Nudler,et al.  The riboswitch control of bacterial metabolism. , 2004, Trends in biochemical sciences.

[2]  V. Méjean,et al.  The leader sequence of the Escherichia coli lysC gene is involved in the regulation of LysC synthesis. , 1998, FEMS Microbiology Letters.

[3]  Nobuo Yamashita,et al.  Thiamine‐regulated gene expression of Aspergillus oryzae thiA requires splicing of the intron containing a riboswitch‐like domain in the 5′‐UTR , 2003, FEBS letters.

[4]  R. Breaker,et al.  Adenine riboswitches and gene activation by disruption of a transcription terminator , 2004, Nature Structural &Molecular Biology.

[5]  Evgeny Nudler,et al.  Sensing Small Molecules by Nascent RNA A Mechanism to Control Transcription in Bacteria , 2002, Cell.

[6]  N. Majdalani,et al.  Regulatory roles for small RNAs in bacteria. , 2003, Current opinion in microbiology.

[7]  R. Breaker,et al.  An mRNA structure that controls gene expression by binding FMN , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Nielsen.  A conserved RNA structure element involved in the regulation of bacterial riboflavin synthesis genes , 2022 .

[9]  Ali Nahvi,et al.  Genetic control by a metabolite binding mRNA. , 2002, Chemistry & biology.

[10]  M Yarus,et al.  Diversity of oligonucleotide functions. , 1995, Annual review of biochemistry.

[11]  T. Henkin,et al.  Transcription termination control of the S box system: Direct measurement of S-adenosylmethionine by the leader RNA , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[12]  R. Breaker,et al.  Control of gene expression by a natural metabolite-responsive ribozyme , 2004, Nature.

[13]  Ali Nahvi,et al.  An mRNA structure that controls gene expression by binding S-adenosylmethionine , 2003, Nature Structural Biology.

[14]  Jeffrey E. Barrick,et al.  Riboswitches Control Fundamental Biochemical Pathways in Bacillus subtilis and Other Bacteria , 2003, Cell.

[15]  Margaret S. Ebert,et al.  An mRNA structure in bacteria that controls gene expression by binding lysine. , 2003, Genes & development.

[16]  Gary D. Stormo,et al.  Do mRNAs act as direct sensors of small molecules to control their expression? , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[17]  S. Kochhar,et al.  Lysine-induced premature transcription termination in the lysC operon of Bacillus subtilis. , 1996, Microbiology.

[18]  T. Henkin,et al.  The L box regulon: Lysine sensing by leader RNAs of bacterial lysine biosynthesis genes , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Andrey A Mironov,et al.  Regulation of the vitamin B12 metabolism and transport in bacteria by a conserved RNA structural element. , 2003, RNA.

[20]  M. Gelfand,et al.  A conserved RNA structure element involved in the regulation of bacterial riboflavin synthesis genes. , 1999, Trends in genetics : TIG.

[21]  Ronald R. Breaker,et al.  Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression , 2002, Nature.

[22]  D. Bartel.  MicroRNAs Genomics, Biogenesis, Mechanism, and Function , 2004, Cell.

[23]  R R Breaker,et al.  Relationship between internucleotide linkage geometry and the stability of RNA. , 1999, RNA.

[24]  R. Breaker,et al.  Genetic Control by Metabolite‐Binding Riboswitches , 2003, Chembiochem : a European journal of chemical biology.

[25]  D. Patel,et al.  Adaptive recognition by nucleic acid aptamers. , 2000, Science.

[26]  J. Miranda-Ríos,et al.  A conserved RNA structure (thi box) is involved in regulation of thiamin biosynthetic gene expression in bacteria , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R R Breaker,et al.  Allosteric nucleic acid catalysts. , 2000, Current opinion in structural biology.

[28]  M. Gelfand,et al.  Regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation. , 2002, Nucleic acids research.

[29]  Vitaly Epshtein,et al.  The riboswitch-mediated control of sulfur metabolism in bacteria , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[30]  T. Henkin,et al.  tRNA-mediated transcription antitermination in vitro: Codon–anticodon pairing independent of the ribosome , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[31]  R. Kadner,et al.  Adenosylcobalamin inhibits ribosome binding to btuB RNA. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[32]  Jeffrey E. Barrick,et al.  Metabolite-binding RNA domains are present in the genes of eukaryotes. , 2003, RNA.

[33]  M. Gelfand,et al.  Regulation of lysine biosynthesis and transport genes in bacteria: yet another RNA riboswitch? , 2003, Nucleic acids research.

[34]  P. Cossart,et al.  An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes , 2002, Cell.

[35]  Jeffrey E. Barrick,et al.  Coenzyme B12 riboswitches are widespread genetic control elements in prokaryotes. , 2004, Nucleic acids research.