Enhanced response to antibody binding in engineered beta-galactosidase enzymatic sensors.
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Antonio Villaverde | Neus Ferrer-Miralles | N. Ferrer-Miralles | A. Villaverde | F. Sobrino | Esther Blanco | Francisco Sobrino | E. Blanco | Jordi X Feliu | Daniel Cazorla | J. X. Feliu | D. Cazorla
[1] A. Villaverde,et al. Enhanced production of pL-controlled recombinant proteins and plasmid stability in Escherichia coli RecA+ strains. , 1993, Journal of biotechnology.
[2] Miguel Ángel Martínez,et al. A single amino acid substitution affects multiple overlapping epitopes in the major antigenic site of foot-and-mouth disease virus of serotype C. , 1990, The Journal of general virology.
[3] J. Sambrook,et al. Molecular Cloning: A Laboratory Manual , 2001 .
[4] Patrice Soumillion,et al. Engineering a regulatable enzyme for homogeneous immunoassays , 1999, Nature Biotechnology.
[5] Miguel Ángel Martínez,et al. Evolution of the capsid protein genes of foot-and-mouth disease virus: antigenic variation without accumulation of amino acid substitutions over six decades , 1992, Journal of virology.
[6] Jeffrey H. Miller. Experiments in molecular genetics , 1972 .
[7] K. Steimer,et al. Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2). , 1985, Science.
[8] A. Villaverde,et al. Distinct mechanisms of antibody‐mediated enzymatic reactivation in β‐galactosidase molecular sensors , 1998, FEBS letters.
[9] A. Villaverde,et al. Optimized release of recombinant proteins by ultrasonication of E. coli cells. , 1998, Biotechnology and bioengineering.
[10] A. Cheung,et al. Comparison of the major antigenic determinants of different serotypes of foot-and-mouth disease virus , 1983, Journal of virology.
[11] J. Saiz,et al. Heterotypic lymphoproliferative response in pigs vaccinated with foot-and-mouth disease virus. Involvement of isolated capsid proteins. , 1992, The Journal of general virology.
[12] R. Tsien,et al. Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin , 1997, Nature.
[13] J. Nelson,et al. Diagnosis of AIDS by using a 12-amino acid peptide representing an immunodominant epitope of the human immunodeficiency virus. , 1987, The Journal of infectious diseases.
[14] J. Corchero,et al. The position of the heterologous domain can influence the solubility and proteolysis of beta-galactosidase fusion proteins in E. coli. , 1996, Journal of biotechnology.
[15] N. Doi,et al. Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution , 1999, FEBS letters.
[16] A. Villaverde,et al. Engineering of solvent‐exposed loops in Escherichia coli β‐galactosidase , 1998 .
[17] Detection of molecular interactions by using a new peptide-displaying bacteriophage biosensor. , 1999, Biochemical and biophysical research communications.
[18] F. Studier,et al. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. , 1986, Journal of molecular biology.
[19] A. Martinez-Arias,et al. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. , 1983, Methods in enzymology.
[20] B. Matthews,et al. Three-dimensional structure of β-galactosidase from E. coli. , 1994, Nature.
[21] A. Villaverde,et al. β-Galactosidase Enzymatic Activity as a Molecular Probe to Detect Specific Antibodies* , 1996, The Journal of Biological Chemistry.
[22] E. Domingo,et al. Multiple genetic variants arise in the course of replication of foot-and-mouth disease virus in cell culture. , 1983, Virology.
[23] F. Sobrino,et al. Foot-and-mouth disease virus: a long known virus, but a current threat. , 2001, Veterinary research.
[24] H. Blöcker,et al. Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region. , 1987, Gene.
[25] J. Marvin,et al. Protein engineering and the development of generic biosensors. , 1998, Trends in biotechnology.
[26] J. Corchero,et al. Reversible activation of a cryptic cleavage site within E. coli beta-galactosidase in beta-galactosidase fusion proteins. , 1997, Biochimica et biophysica acta.
[27] A. Villaverde,et al. Successful mimicry of a complex viral antigen by multiple peptide insertions in a carrier protein , 2000, FEBS letters.
[28] D. Stuart,et al. Flexibility of the major antigenic loop of foot-and-mouth disease virus bound to a Fab fragment of a neutralising antibody: structure and neutralisation. , 1999, Virology.
[29] A. Ullmann. One-step purification of hybrid proteins which have β-galactosidase activity , 1984 .
[30] Insertion of a 27 amino acid viral peptide in different zones of Escherichia coli beta-galactosidase: effects on the enzyme activity. , 1994, FEMS microbiology letters.
[31] Mark L. Pearson,et al. Complete nucleotide sequence of the AIDS virus, HTLV-III , 1985, Nature.