Dynamic diffusion tensor imaging of spinal cord contusion: A canine model

This study aimed to explore the dynamic diffusion tensor imaging (DTI) of changes in spinal cord contusion using a canine model of injury involving rostral and caudal levels. In this study, a spinal cord contusion model was established in female dogs using a custom‐made weight‐drop lesion device. DTI was performed on dogs with injured spinal cords (n=7) using a Siemens 3.0T MRI scanner at pre‐contusion and at 3 h, 24 h, 6 weeks and 12 weeks post‐injury. The tissue sections were stained for immunohistochemical analysis. Canine models of spinal cord contusion were created successfully using the weight‐drop lesion device. The fractional anisotropy (FA) value of lesion epicenter decreased, while the apparent diffusion coefficient (ADC), mean diffusivity (MD), and radial diffusivity (RD) values increased, and the extent of the curve was apparent gradually. The site and time affected the DTI parameters significantly in the whole spinal cord, ADC (site, P < 0.001 and time, P = 0.077, respectively); FA (site, P < 0.001 and time, P = 0.002, respectively). Immunohistological analysis of GFAP and NF revealed the pathologic changes of reactive astrocytes and axons, as well as the cavity and glial scars occurring during chronic SCI. DTI is a sensitive and noninvasive imaging tool useful to assess edema, hemorrhage, cavity formation, structural damage and reconstruction of axon, and myelin in dogs. The DTI parameters after contusion vary. However, the curves of ADC, MD, and RD were nearly similar and the FA curve was distinct. All the DTI parameters were affected by distance and time.

[1]  Giovanni Schifitto,et al.  Simulation of changes in diffusion related to different pathologies at cellular level after traumatic brain injury , 2016, Magnetic resonance in medicine.

[2]  Alexander Sasha Rabchevsky,et al.  Serial Diffusion Tensor Imaging In Vivo Predicts Long-Term Functional Recovery and Histopathology in Rats following Different Severities of Spinal Cord Injury. , 2016, Journal of neurotrauma.

[3]  L. Du,et al.  Intramedullary pressure changes in rats after spinal cord injury , 2016, Spinal Cord.

[4]  J. Rao,et al.  Longitudinal study on diffusion tensor imaging and diffusion tensor tractography following spinal cord contusion injury in rats , 2016, Neuroradiology.

[5]  Allan R. Martin,et al.  Translating state-of-the-art spinal cord MRI techniques to clinical use: A systematic review of clinical studies utilizing DTI, MT, MWF, MRS, and fMRI , 2015, NeuroImage: Clinical.

[6]  John C Gore,et al.  Multiparametric MRI reveals dynamic changes in molecular signatures of injured spinal cord in monkeys , 2015, Magnetic resonance in medicine.

[7]  L. White,et al.  Correlation of Diffusion Tensor Imaging Parameters in the Canine Brain , 2015, The neuroradiology journal.

[8]  F. Wang,et al.  Determination of the ideal rat model for spinal cord injury by diffusion tensor imaging , 2014, Neuroreport.

[9]  Sejong Oh,et al.  A Machine Learning Approach for Specification of Spinal Cord Injuries Using Fractional Anisotropy Values Obtained from Diffusion Tensor Images , 2014, Comput. Math. Methods Medicine.

[10]  S. Kurpad,et al.  Ex vivo diffusion tensor imaging of spinal cord injury in rats of varying degrees of severity. , 2013, Journal of neurotrauma.

[11]  P. Dziallas,et al.  Evaluation of normal appearing spinal cord by diffusion tensor imaging, fiber tracking, fractional anisotropy, and apparent diffusion coefficient measurement in 13 dogs , 2013, Acta Veterinaria Scandinavica.

[12]  A. Tipold,et al.  Quantitative Magnetic Resonance Imaging Characteristics: Evaluation of Prognostic Value in the Dog as a Translational Model for Spinal Cord Injury , 2012, Journal of spinal disorders & techniques.

[13]  M. Schachner,et al.  Transplanted L1 expressing radial glia and astrocytes enhance recovery after spinal cord injury. , 2011, Journal of neurotrauma.

[14]  J. H. Kim,et al.  Diffusion tensor imaging and fiber tractography in cervical compressive myelopathy: preliminary results , 2011, Skeletal Radiology.

[15]  X. Bian,et al.  Glial scar and neuroregeneration: histological, functional, and magnetic resonance imaging analysis in chronic spinal cord injury. , 2010, Journal of neurosurgery. Spine.

[16]  Brian D. Schmit,et al.  Lesion growth and degeneration patterns measured using diffusion tensor 9.4-T magnetic resonance imaging in rat spinal cord injury. , 2010, Journal of neurosurgery. Spine.

[17]  A. Webb,et al.  Spinal cord injury II: Prognostic indicators, standards of care, and clinical trials. , 2010, The Canadian veterinary journal = La revue veterinaire canadienne.

[18]  M. Xiangshui,et al.  3 T magnetic resonance diffusion tensor imaging and fibre tracking in cervical myelopathy. , 2010, Clinical radiology.

[19]  C. Vorhees,et al.  Effect of a neurotoxic dose regimen of (+)-methamphetamine on behavior, plasma corticosterone, and brain monoamines in adult C57BL/6 mice. , 2010, Neurotoxicology and teratology.

[20]  A. Mayer,et al.  A prospective diffusion tensor imaging study in mild traumatic brain injury , 2010, Neurology.

[21]  W. Chiu,et al.  Review Paper: Epidemiology of Traumatic Spinal Cord Injury: Comparisons Between Developed and Developing Countries , 2010, Asia-Pacific journal of public health.

[22]  W. Masri,et al.  A review of published reports on neuroprotection in spinal cord injury , 2009, Spinal Cord.

[23]  B. Ellingson,et al.  Ex vivo diffusion tensor imaging and quantitative tractography of the rat spinal cord during long‐term recovery from moderate spinal contusion , 2008, Journal of magnetic resonance imaging : JMRI.

[24]  P. Fillard,et al.  Diffusion Tensor Imaging Characteristics of the Corpus Callosum in Mild, Moderate, and Severe Traumatic Brain Injury , 2008, American Journal of Neuroradiology.

[25]  H. Levin,et al.  Diffusion tensor imaging of acute mild traumatic brain injury in adolescents , 2008, Neurology.

[26]  G. Johnson,et al.  Short-term DTI predictors of cognitive dysfunction in mild traumatic brain injury , 2008, Brain injury.

[27]  D. Holtzman,et al.  Diffusion Tensor Imaging Reliably Detects Experimental Traumatic Axonal Injury and Indicates Approximate Time of Injury , 2007, The Journal of Neuroscience.

[28]  Derick R. Peterson,et al.  Diffusion tensor imaging detects clinically important axonal damage after mild traumatic brain injury: a pilot study. , 2007, Journal of neurotrauma.

[29]  Joong Hee Kim,et al.  Diffusion tensor imaging predicts hyperacute spinal cord injury severity. , 2007, Journal of neurotrauma.

[30]  K. Hasan,et al.  In vivo serial diffusion tensor imaging of experimental spinal cord injury , 2006, Journal of neuroscience research.

[31]  R. Franklin,et al.  Clinical canine spinal cord injury provides an opportunity to examine the issues in translating laboratory techniques into practical therapy , 2006, Spinal Cord.

[32]  Jun Yoshino,et al.  Demyelination increases radial diffusivity in corpus callosum of mouse brain , 2005, NeuroImage.

[33]  T. Azuma,et al.  New canine spinal cord injury model free from laminectomy. , 2005 .

[34]  A. Krassioukov,et al.  A global perspective on spinal cord injury epidemiology. , 2004, Journal of neurotrauma.

[35]  W. Frawley,et al.  Percutaneous translumbar spinal cord compression injury in dogs from an angioplasty balloon: MR and histopathologic changes with balloon sizes and compression times. , 2004, AJNR. American journal of neuroradiology.

[36]  Mark Ellisman,et al.  Absence of Glial Fibrillary Acidic Protein and Vimentin Prevents Hypertrophy of Astrocytic Processes and Improves Post-Traumatic Regeneration , 2004, The Journal of Neuroscience.

[37]  A. Sorensen,et al.  Diffusion tensor imaging as potential biomarker of white matter injury in diffuse axonal injury. , 2004, AJNR. American journal of neuroradiology.

[38]  A. Privat,et al.  Axonal plasticity and functional recovery after spinal cord injury in mice deficient in both glial fibrillary acidic protein and vimentin genes , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[39]  D. Graham,et al.  Post-acute alterations in the axonal cytoskeleton after traumatic axonal injury. , 2003, Journal of neurotrauma.

[40]  Phil M E Waite,et al.  Axonal injury in children after motor vehicle crashes: extent, distribution, and size of axonal swellings using beta-APP immunohistochemistry. , 2002, Journal of neurotrauma.

[41]  V. Haughton,et al.  Diffusion tensor MR imaging in diffuse axonal injury. , 2002, AJNR. American journal of neuroradiology.

[42]  Alexander Sasha Rabchevsky,et al.  Efficacy of methylprednisolone therapy for the injured rat spinal cord , 2002, Journal of neuroscience research.

[43]  J. Povlishock,et al.  Pathobiology of traumatically induced axonal injury in animals and man. , 1993, Annals of emergency medicine.

[44]  M. Kulkarni,et al.  Acute spinal cord injury: MR imaging at 1.5 T. , 1987, Radiology.

[45]  R. Griebel,et al.  Acute Spinal Cord Injury in the Rat: Comparison of Three Experimental Techniques , 1983, Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques.

[46]  M. Spatz.  Past and recent BBB studies with particular emphasis on changes in ischemic brain edema: dedicated to the memory of Dr. Igor Klatzo. , 2010, Acta neurochirurgica. Supplement.

[47]  T. Nagaoka,et al.  Diffusion tensor feature in vasogenic brain edema in cats. , 2006, Acta neurochirurgica. Supplement.

[48]  J. Noth,et al.  Gradual loss of myelin and formation of an astrocytic scar during Wallerian degeneration in the human spinal cord. , 2004, Brain : a journal of neurology.

[49]  Wise Young,et al.  Spinal cord contusion models. , 2002, Progress in brain research.

[50]  P. Basser,et al.  MR diffusion tensor spectroscopy and imaging. , 1994, Biophysical journal.