Development and Validation of RP-HPLC Method for the Determination of Moxifloxacin in Presence of Its Degradation Products
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A simple, specific and isocratic reversed phase-high performance liquid chromatography (RP-HPLC) method with UV detection at 294 nm was developed and validated for analysis of Moxifloxacin Hydrochloride (MOXI) in presence of its degradation products. Retention time of the MOXI was found to be 7.8 min. A mobile phase consisting of 10mM sodium phosphate buffer and methanol (60:40 v/v) pH 4.4 at flow rate of 1mL/min was employed in this study. The calibration curves were linear with regression coefficient (r2) of 0.999. The method was validated in accordance with International conference on harmonization (ICH) guidelines. The proposed method was found sensitive, specific and was successfully applied for the estimation of MOXI in pharmaceutical formulations (eye drop).
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