Automated image analysis of atomic force microscopy images of rotavirus particles.

A variety of biological samples can be imaged by the atomic force microscope (AFM) under environments that range from vacuum to ambient to liquid. Generally imaging is pursued to evaluate structural features of the sample or perhaps identify some structural changes in the sample that are induced by the investigator. In many cases, AFM images of sample features and induced structural changes are interpreted in general qualitative terms such as markedly smaller or larger, rougher, highly irregular, or smooth. Various manual tools can be used to analyze images and extract more quantitative data, but this is usually a cumbersome process. To facilitate quantitative AFM imaging, automated image analysis routines are being developed. Viral particles imaged in water were used as a test case to develop an algorithm that automatically extracts average dimensional information from a large set of individual particles. The extracted information allows statistical analyses of the dimensional characteristics of the particles and facilitates interpretation related to the binding of the particles to the surface. This algorithm is being extended for analysis of other biological samples and physical objects that are imaged by AFM.

[1]  Linda G. Shapiro,et al.  Computer and Robot Vision , 1991 .

[2]  W. Häberle,et al.  In situ investigations of single living cells infected by viruses. , 1992, Ultramicroscopy.

[3]  T. Thundat,et al.  Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[4]  C. le Grimellec,et al.  Imaging of the membrane surface of MDCK cells by atomic force microscopy. , 1994, Biophysical journal.

[5]  M. Radmacher,et al.  Protein tracking and detection of protein motion using atomic force microscopy. , 1996, Biophysical journal.

[6]  J. Castle,et al.  Enhanced morphological reconstruction of SPM images , 1998 .

[7]  P K Hansma,et al.  Direct observation of enzyme activity with the atomic force microscope. , 1994, Science.

[8]  C. Siegerist,et al.  Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope , 1992, Science.

[9]  J. Hoh,et al.  Slow cellular dynamics in MDCK and R5 cells monitored by time-lapse atomic force microscopy. , 1994, Biophysical journal.

[10]  M. Miles,et al.  Atomic force microscopy of the myosin molecule. , 1995, Biophysical journal.

[11]  Paul K. Hansma,et al.  Imaging adhesion forces and elasticity of lysozyme adsorbed on mica with the atomic force microscope , 1994 .

[12]  C. Bustamante,et al.  Circular DNA molecules imaged in air by scanning force microscopy. , 1992, Biochemistry.

[13]  M. Radmacher,et al.  From molecules to cells: imaging soft samples with the atomic force microscope. , 1992, Science.

[14]  Patrick A. Gerin,et al.  Direct Probing of the Surface Ultrastructure and Molecular Interactions of Dormant and Germinating Spores ofPhanerochaete chrysosporium , 1999, Journal of bacteriology.

[15]  Stuart M. Lindsay,et al.  Imaging DNA molecules chemically bound to a mica surface , 1992, Photonics West - Lasers and Applications in Science and Engineering.

[16]  M. Radmacher,et al.  Bacterial turgor pressure can be measured by atomic force microscopy. , 2000, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.

[17]  Pierre Soille,et al.  Morphological Image Analysis , 1999 .

[18]  J. Patton,et al.  Rotavirus RNA replication: single-stranded RNA extends from the replicase particle. , 1990, The Journal of general virology.

[19]  M J Doktycz,et al.  AFM imaging of bacteria in liquid media immobilized on gelatin coated mica surfaces. , 2003, Ultramicroscopy.

[20]  P K Hansma,et al.  Escherichia coli RNA polymerase activity observed using atomic force microscopy. , 1997, Biochemistry.

[21]  Luc Vincent,et al.  Watersheds in Digital Spaces: An Efficient Algorithm Based on Immersion Simulations , 1991, IEEE Trans. Pattern Anal. Mach. Intell..

[22]  M. Radmacher,et al.  Granula motion and membrane spreading during activation of human platelets imaged by atomic force microscopy. , 1994, Biophysical journal.

[23]  C Rotsch,et al.  Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study. , 2000, Biophysical journal.

[24]  J. Hoh,et al.  Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy , 1996 .

[25]  P. Haydon,et al.  Actin filament dynamics in living glial cells imaged by atomic force microscopy. , 1992, Science.

[26]  T. Thundat,et al.  Imaging isolated strands of DNA molecules by atomic force microscopy. , 1992, Ultramicroscopy.

[27]  Job Ubbink,et al.  Imaging of lactic acid bacteria with AFM--elasticity and adhesion maps and their relationship to biological and structural data. , 2003, Ultramicroscopy.

[28]  L. Björck,et al.  A scanning force microscopy study of human serum albumin and porcine pancreas trypsin adsorption on mica surfaces , 1995 .