Genetic analysis of phosphoprotein and matrix protein of rabies viruses isolated in Brazil.

To investigate the genetic characteristics of phosphoprotein (P) and matrix protein (M) genes of variable rabies virus (RV) prevalent in Brazil, the authors genetically characterized the P and M genes from 30 Brazilian RV field isolates. Phylogenetic analysis based on the P and M genes revealed the presence of six RV variants that consisted primarily of three insectivorous bats, the vampire bat, dog and fox in Brazil. Specific amino acid substitutions corresponding to these phylogenetic lineages were observed, with Asp(42) and Glu(62) in the P protein found to be characteristic of Brazilian chiroptera- and carnivora-related RVs, respectively. Amino acid sequence motifs predicted to associate with a viral function in the P and M proteins were conserved among Brazilian RV variants.

[1]  Y. Kobayashi,et al.  Phylogenetic characterization of rabies virus isolates from Carnivora in Brazil. , 2007, Journal of Veterinary Medical Science.

[2]  N. Ito,et al.  Involvement of nucleoprotein, phosphoprotein, and matrix protein genes of rabies virus in virulence for adult mice. , 2007, Virus research.

[3]  I. Kotait,et al.  Bat-transmitted Human Rabies Outbreaks, Brazilian Amazon , 2006, Emerging infectious diseases.

[4]  Y. Kobayashi,et al.  Molecular epidemiology of rabies from Maranhão and surrounding states in the northeastern region of Brazil , 2006, Archives of Virology.

[5]  S. Nadin-Davis,et al.  The molecular epidemiology of rabies associated with chiropteran hosts in Mexico. , 2006, Virus research.

[6]  S. Finke,et al.  Inhibition of Interferon Signaling by Rabies Virus Phosphoprotein P: Activation-Dependent Binding of STAT1 and STAT2 , 2006, Journal of Virology.

[7]  Y. Kobayashi,et al.  Genetic and phylogenetic characterization of rabies virus isolates from wildlife and livestock in Paraiba, Brazil. , 2006, Acta virologica.

[8]  Y. Kobayashi,et al.  Molecular epidemiological analysis of bat rabies viruses in Brazil. , 2005, The Journal of veterinary medical science.

[9]  S. Finke,et al.  Identification of the Rabies Virus Alpha/Beta Interferon Antagonist: Phosphoprotein P Interferes with Phosphorylation of Interferon Regulatory Factor 3 , 2005, Journal of Virology.

[10]  A. Nishizono,et al.  Comparative sequence analysis of the M gene among rabies virus strains and its expression by recombinant vaccinia virus , 1993, Virus Genes.

[11]  Y. Ito,et al.  Rabies virus M protein expressed inEscherichia coli and its regulatory role in virion-associated transcriptase activity , 2005, Archives of Virology.

[12]  I. Kurane,et al.  Genetic characterization of rabies viruses isolated from frugivorous bat (Artibeus spp.) in Brazil. , 2004, The Journal of veterinary medical science.

[13]  I. Kurane,et al.  Genetic and phylogenetic analysis of glycoprotein of rabies virus isolated from several species in Brazil. , 2004, The Journal of veterinary medical science.

[14]  J. Estaquier,et al.  Lyssavirus Matrix Protein Induces Apoptosis by a TRAIL-Dependent Mechanism Involving Caspase-8 Activation , 2004, Journal of Virology.

[15]  A. Kawai,et al.  Characterization of a Slow‐Migrating Component of the Rabies Virus Matrix Protein Strongly Associated with the Viral Glycoprotein , 2003, Microbiology and immunology.

[16]  S. Finke,et al.  Dissociation of Rabies Virus Matrix Protein Functions in Regulation of Viral RNA Synthesis and Virus Assembly , 2003, Journal of Virology.

[17]  S. Finke,et al.  Rabies virus matrix protein regulates the balance of virus transcription and replication. , 2003, The Journal of general virology.

[18]  S. Nadin-Davis,et al.  Lyssavirus P gene characterisation provides insights into the phylogeny of the genus and identifies structural similarities and diversity within the encoded phosphoprotein. , 2002, Virology.

[19]  I. Kotait,et al.  Antigenic typing of Brazilian rabies virus samples isolated from animals and humans, 1989-2000. , 2002, Revista do Instituto de Medicina Tropical de Sao Paulo.

[20]  R. N. Harty,et al.  Rhabdoviruses and the Cellular Ubiquitin-Proteasome System: a Budding Interaction , 2001, Journal of Virology.

[21]  E. Réal,et al.  Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P. , 2001, The Journal of general virology.

[22]  E. Réal,et al.  Functional Interaction Map of Lyssavirus Phosphoprotein: Identification of the Minimal Transcription Domains , 2001, Journal of Virology.

[23]  D. Garcin,et al.  Longer and Shorter Forms of Sendai Virus C Proteins Play Different Roles in Modulating the Cellular Antiviral Response , 2001, Journal of Virology.

[24]  I. Kurane,et al.  Genetic characterization and geographic distribution of rabies virus isolates in Brazil: identification of two reservoirs, dogs and vampire bats. , 2001, Virology.

[25]  K. W. Lo,et al.  The 8-kDa Dynein Light Chain Binds to Its Targets via a Conserved (K/R)XTQT Motif* , 2001, The Journal of Biological Chemistry.

[26]  H. Raux,et al.  Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain , 2000, Journal of Virology.

[27]  N. Tordo,et al.  Cytoplasmic Dynein LC8 Interacts with Lyssavirus Phosphoprotein , 2000, Journal of Virology.

[28]  S. Nadin-Davis,et al.  A Panel of Monoclonal Antibodies Targeting the Rabies Virus Phosphoprotein Identifies a Highly Variable Epitope of Value for Sensitive Strain Discrimination , 2000, Journal of Clinical Microbiology.

[29]  D. Blondel,et al.  The Phosphoprotein of Rabies Virus Is Phosphorylated by a Unique Cellular Protein Kinase and Specific Isomers of Protein Kinase C , 2000, Journal of Virology.

[30]  R. N. Harty,et al.  A Proline-Rich Motif within the Matrix Protein of Vesicular Stomatitis Virus and Rabies Virus Interacts with WW Domains of Cellular Proteins: Implications for Viral Budding , 1999, Journal of Virology.

[31]  A. Kawai,et al.  Intracellular Behavior of Rabies Virus Matrix Protein (M) Is Determined by the Viral Glycoprotein (G) , 1999, Microbiology and immunology.

[32]  K. Conzelmann,et al.  Matrix Protein of Rabies Virus Is Responsible for the Assembly and Budding of Bullet-Shaped Particles and Interacts with the Transmembrane Spike Glycoprotein G , 1999, Journal of Virology.

[33]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[34]  K. Conzelmann,et al.  Mapping the Interacting Domains between the Rabies Virus Polymerase and Phosphoprotein , 1998, Journal of Virology.

[35]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[36]  Y. Nagai,et al.  The paramyxovirus, Sendai virus, V protein encodes a luxury function required for viral pathogenesis , 1997, The EMBO journal.

[37]  M. Lock,et al.  Identification of a set of proteins (C' and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase. , 1996, Virology.

[38]  伊藤祐司.  Rabies virus M protein expressed in Escherichia coli and its regulatory role in virion-associated transcriptase activity(狂犬病ウイルスM蛋白の大腸菌発現とそのウイルス転写における制御機能) , 1996 .

[39]  K. Chébli,et al.  Translation initiation at alternate in-frame AUG codons in the rabies virus phosphoprotein mRNA is mediated by a ribosomal leaky scanning mechanism , 1995, Journal of virology.

[40]  Y. Gaudin,et al.  In vivo interaction of rabies virus phosphoprotein (P) and nucleoprotein (N): existence of two N-binding sites on P protein. , 1994, The Journal of general virology.

[41]  Z. Fu,et al.  Both the N- and the C-terminal domains of the nominal phosphoprotein of rabies virus are involved in binding to the nucleoprotein. , 1994, Virology.

[42]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[43]  R. Lazzarini,et al.  Stereo images of vesicular stomatitis virus assembly , 1986, Journal of virology.

[44]  C. Hsu,et al.  Site-specific phosphorylation regulates the transcriptive activity of vesicular stomatitis virus NS protein , 1982, Journal of virology.