Pathological and immunocytochemical changes in chronic calcium oxalate nephrolithiasis in the rat.

In the present study, we exposed rats to a crystal-inducing diet (CID) consisting of vitamin D3 and 0.5% ethylene glycol (EG), and we investigated histologically the kidney damage induced by the deposition of calcium oxalate (CaOx) crystals. After 28 days, 50% of the animals had renal CaOx crystals, of which 60% also had small papillary stones. Most crystals were present in the cortex. The occurrence of these crystals coincided with morphological and cytochemical changes: glomerular damage, tubular dilatation and necrosis, and an enlargement of the interstitium. The number of epithelial and interstitial cells positive for the proliferating cell nuclear antigen (PCNA) was increased. Tamm-Horsfall protein (THP) was not only demonstrable in the thick ascending limb of the loop of Henle (TAL), but also frequently in glomeruli, in the proximal tubular epithelium, and in the papilla. In the lumen of the tubular system, it was associated with urinary casts. Reflection contrast microscopy (RCM) showed that the crystals were coated with a thin layer of THP. In spite of the high urinary oxalate concentrations, the above described cellular changes were not observed in CID-fed rats without renal crystals. We conclude, therefore, that in the kidney, the retained CaOx crystals rather than the urinary oxalate ions are responsible for the observed morphological and immunocytochemical changes.

[1]  T. Stijnen,et al.  Experimental nephrolithiasis in rats: the effect of ethylene glycol and vitamin D3 on the induction of renal calcium oxalate crystals. , 1996, Scanning microscopy.

[2]  J. Lieske,et al.  Adhesion of calcium oxalate monohydrate crystals to renal epithelial cells is inhibited by specific anions. , 1995, The American journal of physiology.

[3]  D. Assimos,et al.  Genetic factors in calcium oxalate stone disease. , 1995, The Journal of urology.

[4]  P. Singhal,et al.  Matrix modulates uptake of calcium oxalate crystals and cell growth of renal epithelial cells. , 1995, The Journal of urology.

[5]  E. Worcester.  Urinary calcium oxalate crystal growth inhibitors. , 1994, Journal of the American Society of Nephrology : JASN.

[6]  T. Kurita,et al.  Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone. , 1994, Biochemistry and molecular biology international.

[7]  J. Bates,et al.  Uromodulin (Tamm-Horsfall protein) is a leukocyte adhesion molecule. , 1994, Biochemical and biophysical research communications.

[8]  M. D. de Broe,et al.  Hyperplasia, hypertrophy, and phenotypic alterations in the distal nephron after acute proximal tubular injury in the rat. , 1994, Laboratory investigation; a journal of technical methods and pathology.

[9]  F. Schröder,et al.  Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats. , 1994, Scanning microscopy.

[10]  B. Hess.  Tamm-Horsfall glycoprotein and calcium nephrolithiasis. , 1994, Mineral and electrolyte metabolism.

[11]  D. Denhardt,et al.  Osteopontin: a protein with diverse functions , 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[12]  K. Yoshida,et al.  Sequencing of a Urinary Stone Protein, Identical to α-1 Antitrypsin, Which Lacks 22 Amino Acids , 1993 .

[13]  K. Yoshida,et al.  Sequencing of a urinary stone protein, identical to alpha-one antitrypsin, which lacks 22 amino acids. , 1993, Biochemical and biophysical research communications.

[14]  J. Lieske,et al.  Regulation of renal epithelial cell endocytosis of calcium oxalate monohydrate crystals. , 1993, The American journal of physiology.

[15]  F. Schröder,et al.  An ultrastructural study of experimentally induced microliths in rat proximal and distal tubules. , 1993, The Journal of urology.

[16]  隆宣 山本,et al.  モノクロナール抗体によるTamm-Horsfall glycoproteinの抗原性の解析 , 1993 .

[17]  Saeed R. Khan,et al.  Hyperoxaluria, enzymuria and nephrolithiasis. , 1993, Contributions to nephrology.

[18]  J. Lieske,et al.  Endocytosis of calcium oxalate crystals and proliferation of renal tubular epithelial cells in a patient with type 1 primary hyperoxaluria. , 1992, The Journal of urology.

[19]  R. Wevers,et al.  Measuring urinary glycosaminoglycans in the presence of protein: an improved screening procedure for mucopolysaccharidoses based on dimethylmethylene blue. , 1992, Clinical chemistry.

[20]  E. Hawkins,et al.  Urinary Tamm-Horsfall glycoprotein concentrations in normal and urolithiasis-affected male cats determined by an ELISA. , 1992, Zentralblatt fur Veterinarmedizin. Reihe A.

[21]  R. Hackett,et al.  Urinary enzymes and calcium oxalate urolithiasis. , 1989, The Journal of urology.

[22]  C. Cornelisse,et al.  Nuclear distribution of the Ki-67 antigen during the cell cycle: comparison with growth fraction in human breast cancer cells. , 1989, Cancer research.

[23]  R. Verani,et al.  Granulomatous interstitial nephritis after a jejunoileal bypass: an ultrastructural and histochemical study. , 1989, American journal of nephrology.

[24]  B. Stillman,et al.  Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein , 1987, Nature.

[25]  C. Choi,et al.  Calcium oxalate crystals in the thyroid. Their identification, prevalence, origin, and possible significance. , 1987, American journal of clinical pathology.

[26]  C. Langman,et al.  Increased serum concentrations of 1,25(OH)2 vitamin D in children with fasting hypercalciuria. , 1987, The Journal of pediatrics.

[27]  J. Fransen,et al.  Ultrastructural localization of bronchial antileukoprotease in central and peripheral human airways by a gold-labeling technique using monoclonal antibodies. , 1986, The American review of respiratory disease.

[28]  A. Broadus,et al.  Elevated production rate of 1,25-dihydroxyvitamin D in patients with absorptive hypercalciuria. , 1985, The Journal of clinical endocrinology and metabolism.

[29]  A. Dawnay,et al.  Human Tamm-Horsfall glycoprotein: urinary and plasma levels in normal subjects and patients with renal disease determined by a fully validated radioimmunoassay. , 1985, Clinical science.

[30]  B. Finlayson,et al.  Stone matrix as proteins adsorbed on crystal surfaces: a microscopic study. , 1983, Scanning electron microscopy.

[31]  R. D. Marshall,et al.  Factors affecting excretion of human urinary Tamm-Horsfall glycoprotein. , 1982, Clinical science.

[32]  R. Cotran,et al.  Pathologic localization of Tamm-Horsfall protein in interstitial deposits in renal disease. , 1978, Laboratory investigation; a journal of technical methods and pathology.

[33]  B. Finlayson,et al.  Adsorption of naturally occurring polymers onto calcium oxalate crystal surfaces. , 1977, Investigative urology.

[34]  A. Michael,et al.  Ontogeny of Tamm-Horsfall urinary glycoprotein. , 1974, Laboratory investigation; a journal of technical methods and pathology.

[35]  J. Roberts,et al.  Ethylene glycol toxicity in the monkey. , 1969, Toxicology and applied pharmacology.

[36]  H. David,et al.  [Electronmicroscopic changes of kidney structure after chronical injections of sodium oxalate]. , 1968, Beitrage zur pathologischen Anatomie und zur allgemeinen Pathologie.