Ethoxyresorufin-O-deethylase (EROD) and porphyriainduction in chicken embryo hepatocyte cultures — A new bioassay of PCB, PCDD, and related chemical contamination in wildlife

Abstract Ethoxyresorufin-O-deethylase (EROD) and porphyria induction potencies of extracts from polychlorinated biphenyl (PCB) and 2,3,7,8-tetrachloro dibenzo-p-dioxin (TCDD) contaminated eggs of Herring Gulls, Larus argentatus from the Great Lakes of North America, and Great Blue Herons, Ardea herodias from British Columbia were measured in primary cultures of chicken embryo hepatocytes. The results show that EROD and porphyria induction in these cells have potential for determining the toxic potencies of complex mixtures of halogenated aromatic hydrocarbons (HAHs) extracted from wildlife tissue.

[1]  G. S. Marks,et al.  Chick embryo liver cells maintained in serum-free waymouth MD 705/1 medium , 1976 .

[2]  S. Safe,et al.  Comparative analysis of polychlorinated dibenzo-'p'-dioxin and dibenzofuran congeners in Great Lakes fish extracts by gas chromatography-mass spectrometry and in-vitro enzyme induction activities , 1989 .

[3]  D. Muir,et al.  Polychlorinated dibenzo-p-dioxins and dibenzofurans in marine mammals in the Canadian North. , 1990, Environmental pollution.

[4]  S. Kennedy,et al.  Highly carboxylated porphyrins as a biomarker of polyhalogenated aromatic hydrocarbon exposure in wildlife: Confirmation of their presence in Great Lakes Herring Gull chicks in the early 1970s and important methodological details , 1990 .

[5]  J. Giesy,et al.  Characterization of the H4IIE rat hepatoma cell bioassay as a tool for assessing toxic potency of planar halogenated hydrocarbons in environmental samples , 1991 .

[6]  S. Granick,et al.  Effects by heme, insulin, and serum albumin on heme and protein synthesis in chick embryo liver cells cultured in a chemically defined medium, and a spectrofluorometric assay for porphyrin composition. , 1975, The Journal of biological chemistry.

[7]  J. Giesy,et al.  H4IIE rat hepatoma cell bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents in colonial fish-eating waterbird eggs from the Great Lakes , 1991, Archives of environmental contamination and toxicology.

[8]  S. Safe,et al.  Polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and related compounds: environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs). , 1990, Critical reviews in toxicology.

[9]  R. Norstrom,et al.  A Gel-Permeation/Column Chromatography Cleanup Method for the Determination of CDDs in Animal Tissue , 1986 .

[10]  R. Mayer,et al.  Characteristics of a microsomal cytochrome P-448-mediated reaction. Ethoxyresorufin O-de-ethylation. , 1977, Drug metabolism and disposition: the biological fate of chemicals.

[11]  S. Kennedy,et al.  Porphyria in herring gulls: A biochemical response to chemical contamination of great lakes food chains , 1988 .