论文信息 - Differentiation of collagens I, II, and III by second order susceptibility imaging

Differentiation of collagens I, II, and III by second order susceptibility imaging

Second order susceptibility (SOS) microscopy is used as a contrast mechanism for distinguishing types I and III collagen in tissues. In addition, relative proportion of collagen type was determined by analyzing the histograms of SOS ratios. Our results show that second order susceptibility is an effective metric for differentiating types I and III collagen without extrinsic labeling.

Sheng-Lin Lee | Chen-Yuan Dong | Hsuan-Shu Lee | Chiu-Mei Hsueh | Ya-Der Huang

[1] R. Jaenisch,et al. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[2] P. Howard,et al. Molecular analysis of collagens in bladder fibrosis. , 1998, The Journal of urology.

[3] Guy Cox,et al. 3-dimensional imaging of collagen using second harmonic generation. , 2003, Journal of structural biology.

[4] Chen-Yuan Dong,et al. Multiphoton imaging and quantitative analysis of collagen production by chondrogenic human mesenchymal stem cells cultured in chitosan scaffold. , 2010, Tissue engineering. Part C, Methods.

[5] R. Poertner,et al. Characterization of fibrillar collagen types using multi‐dimensional multiphoton laser scanning microscopy , 2012, International journal of cosmetic science.

[6] Chen-Yuan Dong,et al. The discrimination of type I and type II collagen and the label-free imaging of engineered cartilage tissue. , 2010, Biomaterials.

[7] F. Follis,et al. Osteogenesis imperfecta and cardiovascular diseases. , 1995, The Annals of thoracic surgery.