Ultrahigh-resolution full-field optical coherence microscopy using InGaAs camera.

Full-field optical coherence microscopy (FFOCM) is an interferometric technique for obtaining wide-field microscopic images deep within scattering biological samples. FFOCM has primarily been implemented in the 0.8 mum wavelength range with silicon-based cameras, which may limit penetration when imaging human tissue. In this paper, we demonstrate FFOCM at the wavelength range of 0.9 - 1.4 mum, where optical penetration into tissue is presumably greater owing to decreased scattering. Our FFOCM system, comprising a broadband spatially incoherent light source, a Linnik interferometer, and an InGaAs area scan camera, provided a detection sensitivity of 86 dB for a 2 sec imaging time and an axial resolution of 1.9 mum in water. Images of phantoms, tissue samples, and Xenopus Laevis embryos were obtained using InGaAs and silicon camera FFOCM systems, demonstrating enhanced imaging penetration at longer wavelengths.

[1]  Lingfeng Yu,et al.  Full-color three-dimensional microscopy by wide-field optical coherence tomography. , 2004, Optics express.

[2]  S M Bentzen,et al.  Evaluation of the spatial resolution of a CT scanner by direct analysis of the edge response function. , 1983, Medical physics.

[3]  Kate Grieve,et al.  In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography. , 2005, Optics express.

[4]  M. Akiba,et al.  Full-field optical coherence tomography by two-dimensional heterodyne detection with a pair of CCD cameras. , 2003, Optics letters.

[5]  A C Boccara,et al.  Stroboscopic ultrahigh-resolution full-field optical coherence tomography. , 2005, Optics letters.

[6]  S L Jacques,et al.  Optical properties of intralipid: A phantom medium for light propagation studies , 1992, Lasers in surgery and medicine.

[7]  H. J. van Staveren,et al.  Light scattering in Intralipid-10% in the wavelength range of 400-1100 nm. , 1991, Applied optics.

[8]  J. Schmitt,et al.  Optical-coherence tomography of a dense tissue: statistics of attenuation and backscattering. , 1994, Physics in medicine and biology.

[9]  Renu Tripathi,et al.  Spectral shaping for non-Gaussian source spectra in optical coherence tomography. , 2002, Optics letters.

[10]  C. Boccara,et al.  Ultrahigh-resolution full-field optical coherence tomography. , 2004, Applied optics.

[11]  Harald Sattmann,et al.  A thermal light source technique for optical coherence tomography , 2000 .

[12]  Antonello De Martino,et al.  Full-field optical coherence tomography with thermal light. , 2002, Applied optics.

[13]  D T Delpy,et al.  Evaluation of spatial resolution as a function of thickness for time-resolved optical imaging of highly scattering media. , 1997, Medical physics.

[14]  A. Boccara,et al.  Thermal-light full-field optical coherence tomography. , 2002, Optics letters.

[15]  H Saint-Jalmes,et al.  Full-field optical coherence microscopy. , 1998, Optics letters.

[16]  A. Boccara,et al.  High-resolution full-field optical coherence tomography with a Linnik microscope. , 2002, Applied optics.

[17]  Yuuki Watanabe,et al.  Full-field optical coherence tomography by achromatic phase shifting with a rotating polarizer. , 2005, Applied optics.

[18]  R. Anderson,et al.  The optics of human skin. , 1981, The Journal of investigative dermatology.

[19]  G S Kino,et al.  Mirau correlation microscope. , 1990, Applied optics.

[20]  N Tsurumachi,et al.  Wide-field optical coherence tomography: imaging of biological tissues. , 2002, Applied optics.

[21]  S L Jacques,et al.  Optical properties of rat liver between 350 and 2200 nm. , 1989, Applied optics.