Evaluation of Cotton Genotypes for Drought Tolerance using PEG-6000 Water Stress by Slanting Glass Plate Technique

Twelve Gossypium hirsutum genotypes viz., CPD-750, Sahana, ARB-9701, CNH-120MB, GIHV-218, BS-279, RAH-101, GJHV-477, F-2228, KH-155, L-761 and LH-2076 were evaluated for drought tolerance using PEG6000 water stress at germination stage. The genotypes were subjected to different osmotic potentials (0.0 MPa, 0.05 MPa, -0.148 MPa, -0.295 MPa, -0.491 MPa, -0.735 MPa and -0.846 MPa) by slanting glass plate technique. The genotypes were evaluated for percent seed germination and seedling vigour traits at 12 th and 18 th day showed maximum concentration of PEG-6000 for the germination is 25% (-0.735 MPa) and at this concentration the shoot growth was completely inhibited in all the genotypes. As the PEG concentration increases there was an increase in root to shoot ratio. In conclusion the genotypes which found tolerant to the increased osmotic potential were BS-279, CNH-120MB, GIHV-218 and ARB-9701 whereas, L-761, KH-155 and LH-2076 were found as drought sensitive genotypes.

[1]  W. A. Jatoi,et al.  Evaluation of Cotton Genotypes for Drought Tolerance and Their Correlation Study at Seedling Stage , 2020, Biomedical Journal of Scientific & Technical Research.

[2]  Syamsuddin,et al.  Effect of various polyethylene glycol concentrations on the growth of seedlings of Indigofera zollingeriana , 2019, IOP Conference Series: Earth and Environmental Science.

[3]  Asma,et al.  Polyethylene Glycol Mediated Osmotic Stress Impacts on Growth and Biochemical Aspects of Wheat (Triticum aestivum L.) , 2019, Journal of Crop Science and Biotechnology.

[4]  W. E. Pereira,et al.  Germination of cotton cultivar seeds under water stress induced by polyethyleneglycol-6000 , 2011 .

[5]  Zhang Xueyan,et al.  Drought-tolerance Evaluation of Cotton with PEG Water-stress Method , 2008 .

[6]  L. Radhouane Response of Tunisian autochthonous pearl millet (Pennisetum glaucum (L.) R. Br.) to drought stress induced by polyethylene glycol (PEG) 6000 , 2007 .

[7]  R. Bruno,et al.  Influência de sistemas de culturas, mucuna preta e adubação mineral sobre a qualidade fisiológica de sementes de milho , 2006 .

[8]  W. Pettigrew Physiological Consequences of Moisture Deficit Stress in Cotton , 2004 .

[9]  T. Rodrigues,et al.  Reação enzimática ao estresse salino durante a germinação de estilosantes , 2004 .

[10]  I. Bingham,et al.  The interaction between salinity stress and seed vigour during germination of soyabean seeds , 2003 .

[11]  H. Pirdashti,et al.  Effect of Water Stress on Seed Germination and Seedling Growth of Rice (Oryza sativa L.) Genotypes , 2003 .

[12]  S. C. J. G. A. Perez,et al.  Efeito do estresse hídrico e envelhecimento precoce na viabilidade de sementes osmocondicionadas de paineira (Chorisia speciosa) , 2003 .

[13]  J. Zanuncio,et al.  Water stress and accelerated aging effects on the viability of osmoconditioned Chorisia speciosa seeds , 2003 .

[14]  H. Bohnert,et al.  Plant stress adaptations--making metabolism move. , 1998, Current opinion in plant biology.

[15]  A. Leopold,et al.  Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. , 1992, Plant physiology.

[16]  M. Kaufmann,et al.  The osmotic potential of polyethylene glycol 6000. , 1973, Plant physiology.

[17]  H. M. Taylor,et al.  Water Uptake by Cotton Roots During an Irrigation Cycle , 1971 .

[18]  M. Kaufmann,et al.  The Osmotic Potential of Polyethylene Glycol 60001 , 2022 .