Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Biotec, Bergisch Gladbach, Germany), PLTMax Human Platelet Lysate (Sigma-Aldrich, St. Louis, MO, USA), and MesenCult-hPL media (Stemcell Technologies, Vancouver, BC, Canada). All xeno-free media showed promise as a feasible replacement for animal-derived growth serums. The xeno-free media expanded MSCs more quickly than the FBS-containing medium and also showed great similarity in cell viability and phenotypic expression. In fact, each xeno-free media produced a greater viable cell yield than the standard FBS-containing medium.
[1]
M. Potgieter,et al.
Making the Switch: Alternatives to Fetal Bovine Serum for Adipose-Derived Stromal Cell Expansion
,
2016,
Front. Cell Dev. Biol..
[2]
Z. Tan,et al.
Age-related BMAL1 change affects mouse bone marrow stromal cell proliferation and osteo-differentiation potential
,
2012,
Archives of medical science : AMS.
[3]
C. Jorgensen,et al.
Mesenchymal stem cells in osteoarticular pediatric diseases: an update
,
2012,
Pediatric Research.
[4]
R. Suuronen,et al.
The Potential of Adipose Stem Cells in Regenerative Medicine
,
2011,
Stem Cell Reviews and Reports.
[5]
R. Tuan,et al.
Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis
,
2010,
Journal of cellular physiology.
[6]
J. Goh,et al.
Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: implications for tissue engineering.
,
2010,
Tissue engineering. Part A.
[7]
M. Vemuri,et al.
Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro.
,
2009,
Cytotherapy.
[8]
M. Hedrick,et al.
Fat tissue: an underappreciated source of stem cells for biotechnology.
,
2006,
Trends in biotechnology.
[9]
V. Zachar,et al.
Enabling Technologies for Cell-Based Clinical Translation Comparative Analysis of Media and Supplements on Initiation and Expansion of Adipose-Derived Stem Cells
,
2016
.
[10]
W. Liu,et al.
BMC Cell Biology BioMed Central
,
2007
.