Vacuum-based preservation of surgical specimens: an environmentally-safe step towards a formalin-free hospital.

Formalin as a fixative has no practical substitutes, but is toxic and potentially carcinogenic, so caution of its use in hospitals and elsewhere is mandatory. In our hospital, preservation of surgical specimens into formalin to be transferred to pathology labs was replaced by under-vacuum sealing (UVS) tissues into plastic bags and preservation at 4 degrees C until transfer. Data analysis showed UVS processing to be superior in terms of staff satisfaction and of gross anatomic preservation; no problems in terms of technical feasibility or histopathologic preservation were encountered. Formalin was confined to pathology labs while its use on hospital premises was vastly reduced.

[1]  Anthony Rhodes,et al.  American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. , 2007, Archives of pathology & laboratory medicine.

[2]  N. Goldstein,et al.  Minimum formalin fixation time for consistent estrogen receptor immunohistochemical staining of invasive breast carcinoma. , 2003, American journal of clinical pathology.

[3]  M. Titford,et al.  Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. , 2005, Archives of pathology & laboratory medicine.

[4]  Jeffrey M. Woodbridge.  Econometric Analysis of Cross Section and Panel Data , 2002 .

[5]  Jeffrey M. Wooldridge,et al.  Solutions Manual and Supplementary Materials for Econometric Analysis of Cross Section and Panel Data , 2003 .

[6]  W. Fritz,et al.  WHO‐IARC‐Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. International Agency for Research on Cancer. Bd. 6 u. 7. Lyon 1974 , 1976 .

[7]  Gianni Bussolati,et al.  Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin , 2008, Virchows Archiv.

[8]  D. Dabbs.  Immunohistochemical protocols: back to the future. , 2008, American journal of clinical pathology.

[9]  P. Roller,et al.  Formaldehyde fixation. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  M. Plummer,et al.  International agency for research on cancer. , 2020, Archives of pathology.

[11]  M. C. Harris.  The practice of allergy. , 1954, California medicine.

[12]  Harris Mc.  Practice of Allergy , 1955 .

[13]  Yanxiang Cao,et al.  Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue. , 2009, Archives of pathology & laboratory medicine.

[14]  J. Harrington,et al.  Mortality study of British pathologists. , 1991, American journal of industrial medicine.

[15]  D J Lane,et al.  Formalin asthma in hospital staff. , 1975, British medical journal.

[16]  C. V. van Noesel,et al.  Effects of Processing Delay, Formalin Fixation, and Immunohistochemistry on RNA Recovery From Formalin-fixed Paraffin-embedded Tissue Sections , 2008, Diagnostic molecular pathology : the American journal of surgical pathology, part B.

[17]  Patricia A Stewart,et al.  Mortality from lymphohematopoietic malignancies among workers in formaldehyde industries: the National Cancer Institute Cohort. , 2009, Journal of the National Cancer Institute.