论文信息 - Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly.

Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly.

The pre-mRNA splicing pathway is highly conserved from yeast (S. cerevisiae) to mammals. Of the four snRNPs involved in splicing three (U1, U2 and U4/U6) have been shown to be essential for in vitro splicing. To examine the remaining snRNP, we utilized our previously described genetic procedures (Seraphin and Rosbash, 1989) to prepare yeast extracts depleted of U5 snRNP. The results show that U5 snRNP is necessary for both steps of pre- mRNA splicing and for proper spliceosome assembly, i.e., addition of the U4/U5/U6 triple snRNP. The prior steps of U1 and U2 snRNP addition occur normally in the absence of U5 snRNP.

B. Séraphin | M. Rosbash | N. Abovich

[1] P. Legrain,et al. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding , 1990, Molecular and cellular biology.

[2] B. Séraphin,et al. Early commitment of yeast pre-mRNA to the spliceosome pathway , 1988, Molecular and cellular biology.

[3] J D Beggs,et al. Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing , 1988, Molecular and cellular biology.

[4] A. Krämer,et al. Different small nuclear ribonucleoprotein particles are involved in different steps of splicing complex formation. , 1987, Cold Spring Harbor symposia on quantitative biology.