Temporal variation of hydroxyl radical generation and 8-hydroxy-2′-deoxyguanosine formation by coarse and fine particulate matter

Aims: To determine the induction of 8-hydroxy-2′-deoxyguanosine (8-OHdG) by fine (<2.5 μm) and coarse (10–2.5 μm) particulate matter (PM) sampled over time at one sampling location, and to relate the observed effects to the hydroxyl radical (•OH) generating activities and transition metal content of these samples, and to meteorological parameters. Methods: Weekly samples of coarse and fine PM were analysed for H2O2 dependent •OH formation using electron spin resonance (ESR) and formation of 8-OHdG in calf thymus DNA using an immuno-dotblot assay. Immunocytochemistry was used to determine 8-OHdG formation in A549 human epithelial lung cells. To determine temporal effects, samples from six weeks in summer and six weeks in autumn/winter were compared using ESR and the dotblot assay. Concentrations of leachable V, Cr, Fe, Ni, and Cu were determined by inductively coupled plasma mass spectrometry. Results: Both PM fractions elicited •OH generation as well as 8-OHdG formation in calf thymus DNA and in A549 cells. 8-OHdG formation in the naked DNA was significantly related to •OH generation, but not to metal concentrations except for copper. A significantly higher •OH generation was observed for coarse PM, but not fine PM collected during the autumn/winter season; this was not due to differences in sampled mass or metal content. Specific weather conditions under which increased •OH formation in the coarse mode was observed suggest that other, as yet unknown, anthropogenic components might affect the radical generating capacity of PM. Conclusions: Both coarse and fine PM are able to generate •OH, and induce formation of 8-OHdG. When considered at equal mass, •OH formation shows considerable variability with regard to the fraction of PM, as well as the sampling season. The toxicological implications of this heterogeneity in •OH formation by PM, as can be easily determined by ESR, need further investigation.

[1]  R M Harrison,et al.  Particulate matter in the atmosphere: which particle properties are important for its effects on health? , 2000, The Science of the total environment.

[2]  J. Carter,et al.  Effects of aqueous extracts of PM(10) filters from the Utah valley on human airway epithelial cells. , 1999, The American journal of physiology.

[3]  J Inmon,et al.  Air pollution particles mediated oxidative DNA base damage in a cell free system and in human airway epithelial cells in relation to particulate metal content and bioreactivity. , 2001, Chemical research in toxicology.

[4]  R. Burnett,et al.  Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. , 2002, JAMA.

[5]  H. Ochi,et al.  Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. , 1997, Laboratory investigation; a journal of technical methods and pathology.

[6]  Tingming Shi,et al.  Soluble metals as well as the insoluble particle fraction are involved in cellular DNA damage induced by particulate matter , 2004, Molecular and Cellular Biochemistry.

[7]  B. Finlayson‐Pitts,et al.  Tropospheric air pollution: ozone, airborne toxics, polycyclic aromatic hydrocarbons, and particles. , 1997, Science.

[8]  A. Cohen,et al.  Lung cancer and air pollution. , 1995, Environmental health perspectives.

[9]  D. Dockery,et al.  An association between air pollution and mortality in six U.S. cities. , 1993, The New England journal of medicine.

[10]  C. Monn,et al.  Cytotoxicity and induction of proinflammatory cytokines from human monocytes exposed to fine (PM2.5) and coarse particles (PM10-2.5) in outdoor and indoor air. , 1999, Toxicology and applied pharmacology.

[11]  D. Phillips,et al.  Comparison of the formation of 8-hydroxy-2'-deoxyguanosine and single- and double-strand breaks in DNA mediated by fenton reactions. , 1998, Chemical research in toxicology.

[12]  W. Pryor,et al.  Role of free radicals in the toxicity of airborne fine particulate matter. , 2001, Chemical research in toxicology.

[13]  T. Hei,et al.  Role of oxyradicals in mutagenicity and DNA damage induced by crocidolite asbestos in mammalian cells. , 1999, Cancer research.

[14]  H. Kasai,et al.  Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. , 1997, Mutation research.

[15]  J. Musarrat,et al.  Quantitative immunoanalysis of promutagenic 8-hydroxy-2'-deoxyguanosine in oxidized DNA. , 1994, Carcinogenesis.

[16]  Alfred Wiedensohler,et al.  Atmospheric particle number size distribution in central Europe: Statistical relations to air masses and meteorology , 2001 .

[17]  J. Crapo,et al.  Hydrogen peroxide production by alveolar type II cells, alveolar macrophages, and endothelial cells. , 1991, The American journal of physiology.

[18]  P. S. Gilmour,et al.  Free radical activity and pro-inflammatory effects of particulate air pollution (PM10) in vivo and in vitro. , 1996, Thorax.

[19]  L. Dunemann,et al.  New horizons in human biomonitoring of environmentally and occupationally relevant metals—sector-field ICP-MS versus electrothermal AAS , 2000 .

[20]  P. Borm,et al.  In vitro effects of coal fly ashes: hydroxyl radical generation, iron release, and DNA damage and toxicity in rat lung epithelial cells. , 1999, Inhalation toxicology.

[21]  P. Borm,et al.  Ambient Particulate Matter Induces Oxidative Dna Damage in Lung Epithelial Cells , 2000, Inhalation toxicology.

[22]  J. Carter,et al.  Cytokine production by human airway epithelial cells after exposure to an air pollution particle is metal-dependent. , 1997, Toxicology and applied pharmacology.

[23]  J. Carter,et al.  Effects of aqueous extracts of PM10 filters from the Utah Valley on human airway epithelial cells. , 1999, American journal of physiology. Lung cellular and molecular physiology.

[24]  M. Hermes-Lima.  ROLE OF FREE RADICALS , 2004 .

[25]  W. MacNee,et al.  Free radical activity of PM10: iron-mediated generation of hydroxyl radicals. , 1997, Environmental health perspectives.

[26]  P. Borm,et al.  Cytotoxic and Inflammatory Effects of Coarse and Fine Particulate Matter in Macrophages and Epithelial Cells , 2002 .

[27]  R. Stull.  An Introduction to Boundary Layer Meteorology , 1988 .

[28]  A. Ghio,et al.  Enhancement of 2'-deoxyguanosine hydroxylation and DNA damage by coal and oil fly ash in relation to particulate metal content and availability. , 2000, Chemical research in toxicology.

[29]  R. Devlin,et al.  Inflammatory lung injury after bronchial instillation of air pollution particles. , 2001, American journal of respiratory and critical care medicine.

[30]  K. Donaldson,et al.  Nuclear Factor Kappa-B Activation by Particles and Fibers , 2000, Inhalation toxicology.

[31]  H. Kasai,et al.  Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues , 1987, Nature.

[32]  L. Marnett,et al.  Oxyradicals and DNA damage. , 2000, Carcinogenesis.