Nanostructured electrode based on multi-wall carbon nanotubes/Pt microparticles nanocomposite for electrochemical determination of thiols in rat striatum by high performance liquid chromatography separation.

In this paper, multi-wall carbon nanotubes (MWNTs)/Pt microparticles nanocomposite was prepared by electrodepositing Pt microparticles onto the MWNTs matrix. The surface of glassy carbon electrode was modified with this kind of nanocomposite for measurement of thiols, such as L-cysteine (L-Cys) and glutathione (GSH). Compared with the MWNTs or Pt microparticles modified electrode, the nanocomposite modified electrode exhibited high sensitivity and good stability for detection of thiols. According to the results of experiments, the peak currents of L-Cys and GSH are linear with their concentrations and the detection limits (S/N=3) are 2.9 x 10(-8) mol/L and 4.5 x 10(-8) mol/L, respectively. Coupled with microdialysis, the method has been successfully applied to the determination of these two thiols in rat striatal microdialysates.

[1]  Jiye Jin,et al.  Amperometric determination of glutathione and cysteine on a Pd-IrO2 modified electrode with high performance liquid chromatography in rat brain microdialysate , 2002, Analytical and bioanalytical chemistry.

[2]  Xinjian Huang,et al.  Determination of thiols by capillary electrophoresis with electrochemical detection using a palladium field-decoupler and chemically modified electrodes , 1995 .

[3]  G. S. Owens,et al.  Pulsed electrochemical detection of thiocompounds following microchromatographic separations , 1995 .

[4]  G. Shi,et al.  Study of the interaction of 6-mercaptopurine with protein by microdialysis coupled with LC and electrochemical detection based on functionalized multi-wall carbon nanotubes modified electrode. , 2003, Journal of pharmaceutical and biomedical analysis.

[5]  M. Antonietti,et al.  Mesoporous alumina and aluminosilica with Pd and Pt nanoparticles: Structure and catalytic properties , 2003 .

[6]  J. Richie,et al.  Status of glutathione and other thiols and disulfides in human plasma. , 2000, Biochemical pharmacology.

[7]  D. Rabenstein,et al.  Mercury-based electrochemical detector of liquid chromatography for the detection of glutathione and other sulfur-containing compounds. , 1977, Analytical chemistry.

[8]  L. Pellegrino,et al.  stereotaxic atlas of the rat brain , 1967 .

[9]  L. Baratova,et al.  Determination of biologically active low-molecular-mass thiols in human blood. I. Fast qualitative and quantitative, gradient and isocratic reversed-phase high-performance liquid chromatography with photometric and fluorescence detection. , 2000, Journal of chromatography. A.

[10]  B. Casetta,et al.  Method for the determination of total homocysteine in plasma and urine by stable isotope dilution and electrospray tandem mass spectrometry. , 1999, Clinical chemistry.

[11]  V. Lordi,et al.  Method for Supporting Platinum on Single-Walled Carbon Nanotubes for a Selective Hydrogenation Catalyst , 2001 .

[12]  Hu-lin Li,et al.  Electrocatalytic and Analytical Responses of 10-Methylphenothiazine Toward Reduced Glutathione , 2000 .

[13]  M. MacCoss,et al.  Measurement of homocysteine concentrations and stable isotope tracer enrichments in human plasma. , 1999, Analytical chemistry.

[14]  Otto Zhou,et al.  Fabrication and Properties of Composites of Poly(ethylene oxide) and Functionalized Carbon Nanotubes , 2002 .

[15]  Jian Wang,et al.  Amperometric determination of (R)-salsolinol, (R)-N-methylsalsolinol and monoamine neurotransmitters with liquid chromatography using functionalized multi-wall carbon nanotube modified electrode in Parkinson's patients' cerebrospinal fluid , 2004 .

[16]  M. Biswas.  New polymerization techniques and synthetic methodologies , 2001 .

[17]  E. Gunter,et al.  Rapid and accurate HPLC assay for plasma total homocysteine and cysteine in a clinical laboratory setting. , 1999, Clinical chemistry.

[18]  Linda S. Schadler,et al.  LOAD TRANSFER IN CARBON NANOTUBE EPOXY COMPOSITES , 1998 .

[19]  M. El-Sayed,et al.  Characterization of Pt Nanoparticles Encapsulated in Al2O3 and Their Catalytic Efficiency in Propene Hydrogenation , 2002 .

[20]  Dennis C. Johnson,et al.  Electrocatalytic function of Bi(V) sites in heavily-doped PbO2-film electrodes applied for anodic detection of selected sulfur compounds , 1998 .

[21]  Song Zhang,et al.  Determination of thiocompounds by liquid chromatography with amperometric detection at a Nafion/indium hexacyanoferrate film modified electrode , 1999 .

[22]  Guy Denuault,et al.  Detection of hydrogen peroxide at mesoporous platinum microelectrodes. , 2002, Analytical chemistry.

[23]  W. LaCourse,et al.  Determination of sulfur‐containing antibiotics using high‐performance liquid chromatography with integrated pulsed amperometric detection , 2001 .

[24]  Jian Wang,et al.  Liquid chromatography with amperometric detection using functionalized multi-wall carbon nanotube modified electrode for the determination of monoamine neurotransmitters and their metabolites. , 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[25]  Dennis C. Johnson,et al.  Comparison of pulsed amperometric detection and integrated voltammetric detection for inorganic sulfur compounds in liquid chromatography , 1994 .

[26]  Jing Li,et al.  Nanoscale Electroless Metal Deposition in Aligned Carbon Nanotubes , 1998 .

[27]  C. Niemeyer.  REVIEW Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science , 2022 .

[28]  Jongman Park,et al.  Improved Performance of Unmodified and Cobalt Phthalocyanine‐Modified Carbon‐Kel‐F Composite Electrodes , 1994 .

[29]  V. Amarnath,et al.  A specific HPLC-UV method for the determination of cysteine and related aminothiols in biological samples. , 2003, Talanta.

[30]  Prashant V. Kamat,et al.  Photophysical, photochemical and photocatalytic aspects of metal nanoparticles , 2002 .

[31]  W. LaCourse,et al.  Assay for cephapirin and ampicillin in raw milk by high-performance liquid chromatography--integrated pulsed amperometric detection. , 1998, Analytical chemistry.

[32]  F. Kreuzig,et al.  Rapid automated determination of D-penicillamine in plasma and urine by ion-exchange high-performance liquid chromatography with electrochemical detection using a gold electrode. , 1981, Journal of chromatography.

[33]  T. Chou,et al.  Advances in the science and technology of carbon nanotubes and their composites: a review , 2001 .

[34]  M. Radosavljevic,et al.  Carbon nanotube composites for thermal management , 2002, cond-mat/0205418.

[35]  A Wang,et al.  Determination of thiols following their separation by CZE with amperometric detection at a carbon electrode. , 2000, Journal of pharmaceutical and biomedical analysis.

[36]  C. Mirkin,et al.  Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection. , 2002, Science.

[37]  N. Setoyama,et al.  Size‐Controlled Synthesis and Catalytic Performance of Pt Nanoparticles in Micro‐ and Mesoporous Silica Prepared Using Supercritical Solvents , 2003 .

[38]  R. Kennedy,et al.  In vivo monitoring of glutathione and cysteine in rat caudate nucleus using microdialysis on-line with capillary zone electrophoresis-laser induced fluorescence detection , 1997, Journal of Neuroscience Methods.

[39]  O. Fatibello‐Filho,et al.  L-Cysteine determination using a polyphenol oxidase-based inhibition flow injection procedure , 1999 .

[40]  Danila Moscone,et al.  Characterisation of Prussian blue modified screen-printed electrodes for thiol detection , 2004 .

[41]  I. Pogribny,et al.  A new HPLC method for the simultaneous determination of oxidized and reduced plasma aminothiols using coulometric electrochemical detection. , 1999, The Journal of nutritional biochemistry.

[42]  O. Gratzl,et al.  Journal of Cerebral Blood Flow and Metabolism Extracellular Antioxidants and Amino Acids in the Cortex of the Rat: Monitoring by Microdialysis of Early Ischemic Changes , 2022 .

[43]  R. Adams,et al.  Determination of reduced glutathione in guinea pig and rat tissue by HPLC with electrochemical detection. , 1978, Life sciences.

[44]  Angel Rubio,et al.  Single‐Walled Carbon Nanotube–Polymer Composites: Strength and Weakness , 2000 .

[45]  Stephen Mann,et al.  Directed Self‐Assembly of Nanoparticles into Macroscopic Materials Using Antibody–Antigen Recognition , 1999 .