Differential expression of histochemical characteristics in the developing olfactory receptor cells in a flatfish, barfin flounder (Verasper moseri).

Differentiation of the histochemical characteristics of the olfactory receptor cells (ORC) was examined by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and calretinin (CR) and lectin histochemistry for Phaseolus vulgaris agglutinin-L (PHA-L) in the developing olfactory epithelium (OE) of the barfin flounder. PGP 9.5 immunoreactivity was diffuse and CR immunoreactivity was restricted at day 7, but these immunoreactivities became intense in the OE toward day 91. Crypt cells were first identified at day 56. PHA-L staining was faint at day 28, but became intense toward day 91. These findings suggest that PGP 9.5-immunopositive cells, CR-immunopositive cells, crypt cells and PHA-L-reactive cells differentiate independently in the developing OE and constitute subsets of the ORC in the OE.

[1]  J. Vega,et al.  The crypt neurons in the olfactory epithelium of the adult zebrafish express TrkA-like immunoreactivity , 2003, Neuroscience Letters.

[2]  B. Zielinski,et al.  Morphology and histochemistry of the peripheral olfactory organ in the round goby, Neogobius melanostomus (Teleostei: Gobiidae) , 2003, Journal of morphology.

[3]  A. Hansen,et al.  Early development of the olfactory organ in sturgeons of the genus Acipenser: a comparative and electron microscopic study , 2003, Anatomy and Embryology.

[4]  H. Wakisaka,et al.  Lectin histochemical study on the olfactory organ of the newt, Cynops pyrrhogaster, revealed heterogeneous mucous environments in a single nasal cavity , 2003, Anatomy and Embryology.

[5]  S. Saito,et al.  Expression patterns of glycoconjugates in the three distinctive olfactory pathways of the clawed frog, Xenopus laevis. , 2000, The Journal of veterinary medical science.

[6]  Kazuaki W. Takahashi,et al.  Fine structure of three types of olfactory organs in Xenopus laevis , 1998, The Anatomical record.

[7]  J. G. Briñón,et al.  Calretinin immunoreactivity in the developing olfactory system of the rainbow trout. , 1997, Brain research. Developmental brain research.

[8]  David H. Evans,et al.  The Physiology of Fishes , 1994 .

[9]  K. Taniguchi,et al.  Immunohistochemical demonstration of protein gene product 9.5 (PGP 9.5) in the primary olfactory system of the rat , 1993, Neuroscience Letters.

[10]  M. Coogan,et al.  Vomeronasal and olfactory nerves of adult and larval bullfrogs: I. Axons and the distribution of their glomeruli , 1990, The Journal of comparative neurology.

[11]  M. Halpern.  The organization and function of the vomeronasal system. , 1987, Annual review of neuroscience.

[12]  A. Fleming,et al.  Vomeronasal and olfactory system modulation of maternal behavior in the rat. , 1979, Science.

[13]  P. Graziadei,et al.  Functional Anatomy of the Mammalian Chemoreceptor System , 1977 .