Supportless electrochemical sensor based on molecularly imprinted polymer modified nanoporous microrod for determination of dopamine at trace level.

In this work, we developed a novel freestanding metallic microrod as working electrode for highly sensitive and selective electrochemical detection of trace dopamine (DA). The electrode was facilely fabricated via first dealloying smooth Au-Ag alloy microrod (AMR) into nanoporous Au-Ag alloy microrod (NPAMR) and further modifying with electro-polymerized molecularly imprinted polymer (MIP). Influencing factors during electro-polymerization process including pH value and molar ratio of monomer to template molecule were optimized. Under the optimal conditions, a linear range from 2 × 10(-13) to 2 × 10(-8)M for measuring DA was obtained with an ultralow detection limit of 7.63 × 10(-14)M (S/N=3). In addition, the MIP-modified electrode (MIP/NPAMR) was successfully employed to test DA in serum and brain samples.

[1]  Guobao Xu,et al.  Simultaneous electrochemical determination of uric acid, dopamine, and ascorbic acid at single-walled carbon nanohorn modified glassy carbon electrode. , 2009, Biosensors & bioelectronics.

[2]  B. Liu,et al.  Dopamine molecularly imprinted electrochemical sensor based on graphene–chitosan composite , 2012 .

[3]  Ying Wang,et al.  Application of graphene-modified electrode for selective detection of dopamine , 2009 .

[4]  Xin Tang,et al.  Conjugated Polymer Nanoparticles for Fluorescence Imaging and Sensing of Neurotransmitter Dopamine in Living Cells and the Brains of Zebrafish Larvae. , 2015, ACS applied materials & interfaces.

[5]  C. Lunte,et al.  End-column amperometric detection in capillary electrophoresis: influence of separation-related parameters on the observed half-wave potential for dopamine and catechol. , 1999, Analytical chemistry.

[6]  Eugenio Vilanova,et al.  A simple and rapid HPLC-MS method for the simultaneous determination of epinephrine, norepinephrine, dopamine and 5-hydroxytryptamine: application to the secretion of bovine chromaffin cell cultures. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[7]  Guohua Jiang,et al.  Preparation of N-doped carbon quantum dots for highly sensitive detection of dopamine by an electrochemical method , 2015 .

[8]  N. Alizadeh,et al.  Electrochemically controlled release of anticancer drug methotrexate using nanostructured polypyrrole modified with cetylpyridinium: Release kinetics investigation , 2014 .

[9]  Wen-Li Jia,et al.  Highly selective and sensitive determination of dopamine using a Nafion/carbon nanotubes coated poly(3-methylthiophene) modified electrode. , 2006, Biosensors & bioelectronics.

[10]  Yingchun Li,et al.  Molecularly imprinted polymer decorated nanoporous gold for highly selective and sensitive electrochemical sensors , 2015, Scientific Reports.

[11]  I. Balevičiūtė,et al.  Evaluation of Theophylline Imprinted Polypyrrole Film , 2015 .

[12]  Wei Xu,et al.  Electrochemical sensor using neomycin-imprinted film as recognition element based on chitosan-silver nanoparticles/graphene-multiwalled carbon nanotubes composites modified electrode. , 2013, Biosensors & bioelectronics.

[13]  N. Alizadeh,et al.  Catalytic Behaviour of Transition Metal Cations In Electrosynthesis and Growth of Nanostructure Conducting Polypyrrole Films On/Between the Passive Cu Interdigital Electrodes: Application In Gas Sensors , 2014 .

[14]  D. Choi,et al.  Simultaneous measurement of serotonin, dopamine and their metabolites in mouse brain extracts by high-performance liquid chromatography with mass spectrometry following derivatization with ethyl chloroformate. , 2013, Biological & pharmaceutical bulletin.

[15]  Weihong Tan,et al.  A ligation-triggered DNAzyme cascade for amplified fluorescence detection of biological small molecules with zero-background signal. , 2011, Journal of the American Chemical Society.

[16]  Liaochuan Jiang,et al.  A highly sensitive nonenzymatic glucose sensor based on CuO nanoparticles-modified carbon nanotube electrode. , 2010, Biosensors & bioelectronics.

[17]  F. Meng,et al.  Sub‐Micrometer‐Thick All‐Solid‐State Supercapacitors with High Power and Energy Densities , 2011, Advanced materials.

[18]  B. Sellergren,et al.  Molecularly Imprinted Polymers via High‐Throughput and Combinatorial Techniques , 2004 .

[19]  Hongying Liu,et al.  Electrogenerated chemiluminescence of Au nanoclusters for the detection of dopamine. , 2011, Analytical chemistry.

[20]  K. Mosbach,et al.  Molecularly imprinted polymers and their use in biomimetic sensors. , 2000, Chemical reviews.

[21]  B. Rezaei,et al.  Fabrication of DNA, o-phenylenediamine, and gold nanoparticle bioimprinted polymer electrochemical sensor for the determination of dopamine. , 2015, Biosensors & bioelectronics.

[22]  Dan Fei,et al.  Smart molecularly imprinted polymers: recent developments and applications. , 2013, Macromolecular rapid communications.

[23]  K. Ho,et al.  Enhancing dopamine detection using a glassy carbon electrode modified with MWCNTs, quercetin, and Nafion. , 2009, Biosensors & bioelectronics.

[24]  S. Jeon,et al.  Modified platinum electrode with phytic acid and single-walled carbon nanotube: Application to the selective determination of dopamine in the presence of ascorbic and uric acids , 2008 .

[25]  R. Wightman,et al.  Detection of dopamine dynamics in the brain. , 1988, Analytical chemistry.

[26]  Yadong Li,et al.  Nanostructuring gold wires as highly durable nanocatalysts for selective reduction of nitro compounds and azides with organosilanes , 2015, Nano Research.

[27]  Naader Alizadeh,et al.  A dual usage smart sorbent/recognition element based on nanostructured conducting molecularly imprinted polypyrrole for simultaneous potential-induced nanoextraction/determination of ibuprofen in biomedical samples by quartz crystal microbalance sensor , 2015 .

[28]  M. Chan-Park,et al.  Synthesis of graphene–carbon nanotube hybrid foam and its use as a novel three-dimensional electrode for electrochemical sensing , 2012 .

[29]  Danbi Tian,et al.  Dopamine sensor based on molecularly imprinted electrosynthesized polymers , 2010 .

[30]  Yang Wang,et al.  Amperometric detection of dopamine in human serum by electrochemical sensor based on gold nanoparticles doped molecularly imprinted polymers. , 2013, Biosensors & bioelectronics.

[31]  Xingyu Jiang,et al.  Gold nanoparticles for the colorimetric and fluorescent detection of ions and small organic molecules. , 2011, Nanoscale.

[32]  Arturo J. Miranda-Ordieres,et al.  Electrochemical sensors based on molecularly imprinted polymers , 2004 .

[33]  T. Chung,et al.  Synthesis of a graphene–carbon nanotube composite and its electrochemical sensing of hydrogen peroxide , 2012 .

[34]  Feng Yu,et al.  Electrochemical biosensor based on graphene oxide-Au nanoclusters composites for L-cysteine analysis. , 2012, Biosensors & bioelectronics.

[35]  Liu Yuan,et al.  Fabrication of highly sensitive and selective electrochemical sensor by using optimized molecularly imprinted polymers on multi-walled carbon nanotubes for metronidazole measurement , 2015 .

[36]  Jianping Li,et al.  A sensitive and selective sensor for dopamine determination based on a molecularly imprinted electropolymer of o-aminophenol , 2009 .

[37]  M. Hows,et al.  High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples , 2004, Journal of Neuroscience Methods.

[38]  Jing Chen,et al.  A novel sensitive electrochemical sensor based on in-situ polymerized molecularly imprinted membranes at graphene modified electrode for artemisinin determination. , 2015, Biosensors & bioelectronics.

[39]  Zhiwei Zhu,et al.  Selective detection of dopamine in the presence of ascorbic acid and uric acid by a carbon nanotubes-ionic liquid gel modified electrode. , 2005, Talanta.

[40]  Franz L Dickert,et al.  Bioimprinted QCM sensors for virus detection—screening of plant sap , 2004, Analytical and Bioanalytical Chemistry.

[41]  K. Mosbach,et al.  The Use of Immobilized Templates-A New Approach in Molecular Imprinting. , 2000, Angewandte Chemie.

[42]  Bang-Ce Ye,et al.  A novel molecular beacon-based method for isothermal detection of sequence-specific DNA via T7 RNA polymerase-aided target regeneration. , 2015, Biosensors & bioelectronics.

[43]  Chen Li,et al.  Imprinted electrochemical sensor for dopamine recognition and determination based on a carbon nanotube/polypyrrole film , 2012 .

[44]  Franz L. Dickert,et al.  The detection of halogenated hydrocarbons via host-guest chemistry―a mass-sensitive sensor study with QMB- and SAW-devices , 1991 .

[45]  N. Alizadeh,et al.  Gas Sensing Ability of a Nanostructured Conducting Polypyrrole Film Prepared by Catalytic Electropolymerization on Cu/Au Interdigital Electrodes , 2013 .

[46]  William R. Heineman,et al.  A nanotube array immunosensor for direct electrochemical detection of antigen–antibody binding , 2007 .

[47]  Langchong He,et al.  Uniformly sized molecularly imprinted polymers for on-line concentration, purification, and measurement of nimodipine in plasma , 2009 .

[48]  Arunas Ramanavicius,et al.  Copper nanoparticle modified carbon electrode for determination of dopamine , 2012 .

[49]  B. Ye,et al.  Lysine acetylproteome analysis suggests its roles in primary and secondary metabolism in Saccharopolyspora erythraea , 2014, Applied Microbiology and Biotechnology.

[50]  Yanbin Shen,et al.  Fabrication of a template-synthesized gold nanorod-modified electrode for the detection of dopamine in the presence of ascorbic acid , 2007 .

[51]  K. Mosbach,et al.  Synthesis of substrate‐selective polymers by host‐guest polymerization , 1981 .

[52]  Xingguo Chen,et al.  A sensitive biosensor for dopamine determination based on the unique catalytic chemiluminescence of metal–organic framework HKUST-1 , 2015 .

[53]  Yi Lu,et al.  Catalytic and molecular beacons for amplified detection of metal ions and organic molecules with high sensitivity. , 2010, Analytical chemistry.

[54]  X. Xia,et al.  Electrochemical sensor based on nitrogen doped graphene: simultaneous determination of ascorbic acid, dopamine and uric acid. , 2012, Biosensors & bioelectronics.

[55]  Aicheng Chen,et al.  Fabrication and electrochemical properties of novel nanoporous platinum network electrodes. , 2004, Chemical communications.

[56]  A. Turner,et al.  Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization. , 2003, Biotechnology and bioengineering.

[57]  Shen-Ming Chen,et al.  Palladium nanoparticles modified electrode for the selective detection of catecholamine neurotransmitters in presence of ascorbic acid. , 2009, Bioelectrochemistry.