Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection

ObjectiveLoop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments.ResultsHere we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field.

[1]  Michael G. Mauk,et al.  Instrument-Free Point-of-Care Molecular Detection of Zika Virus , 2016, Analytical chemistry.

[2]  E. Corbett,et al.  The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification (LAMP) Assay for Tuberculosis Diagnosis in Adults with Chronic Cough in Malawi , 2016, PloS one.

[3]  Gregory L. Damhorst,et al.  Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip from Whole Blood , 2015, Engineering.

[4]  Aldrik H. Velders,et al.  Simple 3D Printed Scaffold‐Removal Method for the Fabrication of Intricate Microfluidic Devices , 2015, Advanced science.

[5]  M. T. McIntosh,et al.  Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses , 2012, Journal of Clinical Microbiology.

[6]  Abdullah S. Ali,et al.  Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar , 2015, Malaria Journal.

[7]  Yasuyoshi Mori,et al.  Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects , 2015, Journal of Microbiology.

[8]  A. Wolff,et al.  A Sensitive, Specific and Simple Loop Mediated Isothermal Amplification Method for Rapid Detection of Campylobacter spp. in Broiler Production , 2019, Front. Microbiol..

[9]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[10]  Y. Mori,et al.  Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases , 2009, Journal of Infection and Chemotherapy.

[11]  T. Notomi,et al.  Loop-mediated isothermal amplification of DNA. , 2000, Nucleic acids research.

[12]  R. Kundapur,et al.  Loop-mediated isothermal amplification: Beyond microbial identification , 2016 .

[13]  J. Gooding,et al.  Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). , 2014, Biosensors & bioelectronics.

[14]  Daniel Cressey,et al.  The DIY electronics transforming research , 2017, Nature.

[15]  M. A. Diallo,et al.  Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites , 2016, Scientific Reports.

[16]  Feng Shi,et al.  Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae , 2019, World journal of microbiology & biotechnology.

[17]  Min-Gon Kim,et al.  A Paper-Based Device for Performing Loop-Mediated Isothermal Amplification with Real-Time Simultaneous Detection of Multiple DNA Targets , 2017, Theranostics.

[18]  A. Wolff,et al.  Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting , 2019, Front. Microbiol..

[19]  N. Horita,et al.  Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis , 2016, Scientific Reports.

[20]  Sara W. Bird,et al.  A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses , 2017, Scientific Reports.