A MORPHOMETRIC INVESTIGATION OF PULMONARY SYMPATHETIC INNERVATION IN NITROFEN-INDUCED CONGENITAL DIAPHRAGMATIC HERNIA RATS

1) Abstract　Pulmonary hypoplasia influences prognosis in congenital diaphragmatic hernia (CDH) . However the abnormality of pulmonary innervation is not clearly understood. We immunohistochemically examined the lungs of CDH model rats with special reference to sympathetic innervation. Pregnant rats were received nitrofen on gestational day 9 and the fetuses were recovered on gestational day 22. Control animals received only olive oil. Fetal lungs were dissected and the weight was measured. Whole mount sections at the level of main bronchus were immunostained using anti‑tyrosine hydroxylase (TH) antibody as a sympathetic marker. The proportion of area of TH‑immunoreactive nerve fi bers relative to the total lung area was calculated. The mean lung weight of CDH aff ected side was signifi cantly lower than that of ipsilateral side of controls. The percentage of TH‑positive area of CDH affected side was significantly lower than that of unaffected side. The reduction of pulmonary sympathetic innervation may play an important role in respiratory morbidity in CDH.

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