Ventrolateral medullary respiratory network participation in the expiration reflex in the cat.

The expiration reflex is a distinct airway defensive response characterized by a brief, intense expiratory effort and coordinated adduction and abduction of the laryngeal folds. This study addressed the hypothesis that the ventrolateral medullary respiratory network participates in the reflex. Extracellular neuron activity was recorded with microelectrode arrays in decerebrated, neuromuscular-blocked, ventilated cats. In 32 recordings (17 cats), 232 neurons were monitored in the rostral (including Bötzinger and pre-Bötzinger complexes) and caudal ventral respiratory group. Neurons were classified by firing pattern, evaluated for spinal projections, functional associations with recurrent laryngeal and lumbar nerves, and firing rate changes during brief, large increases in lumbar motor nerve discharge (fictive expiration reflex, FER) elicited during mechanical stimulation of the vocal folds. Two hundred eight neurons were respiratory modulated, and 24 were nonrespiratory; 104 of the respiratory and 6 of the nonrespiratory-modulated neurons had altered peak firing rates during the FER. Increased firing rates of bulbospinal neurons and expiratory laryngeal premotor and motoneurons during the expiratory burst of FER were accompanied by changes in the firing patterns of putative propriobulbar neurons proposed to participate in the eupneic respiratory network. The results support the hypothesis that elements of the rostral and caudal ventral respiratory groups participate in generating and shaping the motor output of the FER. A model is proposed for the participation of the respiratory network in the expiration reflex.

[1]  J. Lipski,et al.  Extensive monosynaptic inhibition of ventral respiratory group neurons by augmenting neurons in the Bötzinger complex in the cat , 2006, Experimental Brain Research.

[2]  P. Calverley,et al.  Control of breathing , 2005 .

[3]  N. N. Preobrazhenskii,et al.  Responses of bulbospinal neurons during expiration reflex in cats , 2005, Neurophysiology.

[4]  Ulysses J. Balis,et al.  Simulations of a ventrolateral medullary neural network for respiratory rhythmogenesis inferred from spike train cross-correlation , 1994, Biological Cybernetics.

[5]  D. Richter,et al.  Studies on the synaptic interconnection between bulbar respiratory neurones of cats , 1979, Pflügers Archiv.

[6]  Z. Tomori,et al.  Activity of the laryngeal abductor and adductor muscles during cough, expiration and aspiration reflexes in cats. , 2003, Physiological research.

[7]  K. Morris,et al.  Medullary respiratory neurones and control of laryngeal motoneurones during fictive eupnoea and cough in the cat , 2001, The Journal of physiology.

[8]  B G Lindsey,et al.  Functional connectivity among ventrolateral medullary respiratory neurones and responses during fictive cough in the cat , 2000, The Journal of physiology.

[9]  J. Korpáš,et al.  The expiration reflex from the vocal folds. , 2000, Acta physiologica Hungarica.

[10]  I. Poliaček,et al.  Kainic acid lesions to the lateral tegmental field of medulla: effects on cough, expiration and aspiration reflexes in anesthetized cats. , 2000, Physiological research.

[11]  A. Bianchi,et al.  Activity of respiratory laryngeal motoneurons during fictive coughing and swallowing , 2000, Experimental Brain Research.

[12]  K. Shiba,et al.  Multifunctional Laryngeal Motoneurons: an Intracellular Study in the Cat , 1999, The Journal of Neuroscience.

[13]  E. Fetz,et al.  Response patterns and force relations of monkey spinal interneurons during active wrist movement. , 1998, Journal of neurophysiology.

[14]  E. Fetz,et al.  Activity of spinal interneurons and their effects on forearm muscles during voluntary wrist movements in the monkey. , 1998, Journal of neurophysiology.

[15]  B G Lindsey,et al.  Ventrolateral medullary respiratory network and a model of cough motor pattern generation. , 1998, Journal of applied physiology.

[16]  G. Fontana,et al.  Discharge patterns of Bötzinger complex neurons during cough in the cat. , 1998, American journal of physiology. Regulatory, integrative and comparative physiology.

[17]  I. Poliaček,et al.  Effects of medullary midline lesions on cough and other airway reflexes in anaesthetized cats. , 1998, Physiological research.

[18]  K. Morris,et al.  Brainstem respiratory networks and cough. , 1996, Pulmonary pharmacology.

[19]  B G Lindsey,et al.  Long‐term facilitation of phrenic nerve activity in cats: responses and short time scale correlations of medullary neurones. , 1996, The Journal of physiology.

[20]  I. Poliaček,et al.  Laryngeal patency and expiration reflex following focal cold block of the medulla in the cat. , 1996, Physiological research.

[21]  J C Smith,et al.  Pre-Bötzinger complex in the cat. , 1995, Journal of neurophysiology.

[22]  J. Champagnat,et al.  Central control of breathing in mammals: neuronal circuitry, membrane properties, and neurotransmitters. , 1995, Physiological reviews.

[23]  C. Christakos,et al.  Analysis of recurrent laryngeal inspiratory discharges in relation to fast rhythms. , 1994, Journal of neurophysiology.

[24]  B G Lindsey,et al.  Functional connectivity between brain stem midline neurons with respiratory-modulated firing rates. , 1992, Journal of neurophysiology.

[25]  N. N. Preobrazhenskii,et al.  [Responses of bulbo-spinal neurons during the expiration reflex in cats]. , 1991, Neirofiziologiia = Neurophysiology.

[26]  D'iachenko IuE,et al.  [Responses of bulbo-spinal neurons during the expiration reflex in cats]. , 1991 .

[27]  K. Ezure,et al.  Synaptic connections between medullary respiratory neurons and considerations on the genesis of respiratory rhythm , 1990, Progress in Neurobiology.

[28]  M. Corda,et al.  Expiration-related neurons in the caudal ventral respiratory group of the cat: influences of the activation of Bo¨tzinger complex neurons , 1990, Brain Research.

[29]  L. Grélot,et al.  Discharge patterns of laryngeal motoneurones in the cat: an intracellular study , 1990, Brain Research.

[30]  P. Cheney,et al.  Corticomotoneuronal postspike effects in averages of unrectified EMG activity. , 1989, Journal of neurophysiology.

[31]  L. Ayvazian.  Handbook of Physiology. The Respiratory System, Volume IV: Gas Exchange , 1988 .

[32]  B G Lindsey,et al.  Functional associations among simultaneously monitored lateral medullary respiratory neurons in the cat. II. Evidence for inhibitory actions of expiratory neurons. , 1987, Journal of neurophysiology.

[33]  Z Tomori,et al.  Bulbar respiratory activity during defensive airways reflexes in cats. , 1987, Acta physiologica Hungarica.

[34]  J. Dostrovsky,et al.  Projections of the internal branch of the superior laryngeal nerve of the cat , 1986, Brain Research Bulletin.

[35]  T. Sears,et al.  The distribution of monosynaptic connexions from inspiratory bulbospinal neurones to inspiratory motoneurones in the cat. , 1985, The Journal of physiology.

[36]  Activity of bulbar respiratory neurones during cough and other respiratory tract reflexes in cats. , 1985, Physiologia Bohemoslovaca.

[37]  H. Arita.  [Neural control of the respiratory muscles]. , 1984, Kokyu to junkan. Respiration & circulation.

[38]  T. Dick,et al.  Consistency and signal strength of respiratory neuronal activity. , 1983, Journal of neurophysiology.

[39]  S. Sorokin,et al.  The Respiratory System , 1983 .

[40]  M. Mesulam,et al.  Brain stem projections of sensory and motor components of the vagus complex in the cat: II. Laryngeal, tracheobronchial, pulmonary, cardiac, and gastrointestinal branches , 1980, The Journal of comparative neurology.

[41]  J. Korpáš,et al.  Cough and Other Respiratory Reflexes , 1979 .

[42]  Z. Tomori,et al.  Changes in laryngeal motoneurone activity and in laryngeal calibre during the expiration reflex. , 1979, Physiologia Bohemoslovaca.

[43]  E. Kirsten,et al.  A feline decerebration technique with low mortality and long-term homeostasis☆ , 1978 .

[44]  J. Korpáš,et al.  Intraepithelial nerve fibres of the cat larynx and the expiration reflex [proceedings]. , 1978, The Journal of physiology.

[45]  M. I. Cohen,et al.  Synaptic connections between medullary inspiratory neurons and phrenic motoneurons as revealed by cross-correlation. , 1974, Brain research.

[46]  Z. Tomori,et al.  Electroneurographic and pneumotachographic analysis of the expiration reflex. , 1973, Physiologia Bohemoslovaca.

[47]  J. Korpáš.  Differentiation of the expiration and the cough reflex. , 1972, Physiologia Bohemoslovaca.