OLED-based DNA biochip for Campylobacter spp. detection in poultry meat samples.

Integrated biochips are the ideal solution for producing portable diagnostic systems that uncouple diagnosis from centralized laboratories. These portable devices exploit a multi-disciplinary approach, are cost effective and have several advantages including broader accessibility, high sensitivity, quick test results and ease of use. The application of such a device in food safety is considered in this paper. Fluorescence detection of a specific biological probe excited by an optical source is one of the most commonly used methods for quantitative analysis on biochips. In this study, we designed and characterized a miniaturized, highly-sensitive DNA biochip based on a deep-blue organic light-emitting diode. The molecular design of the diode was optimized to excite a fluorophore-conjugated DNA probe and tested using real meat samples to obtain a high sensitivity and specificity against one of the most common poultry meat contaminants: Campylobacter spp. Real samples were analyzed also by classical plate methods and molecular methods to validate the results obtained by the new DNA-biochip. The high sensitivity obtained by the OLED based biochip (0.37ng/μl) and the short time required for the results (about 24h) indicate the usefulness of the system.

[1]  Ian Papautsky,et al.  High-sensitivity, disposable lab-on-a-chip with thin-film organic electronics for fluorescence detection. , 2008, Lab on a chip.

[2]  Lucilla Iacumin,et al.  Rapid detection and differentiation of important Campylobacter spp. in poultry samples by dot blot and PCR. , 2014, Food microbiology.

[3]  R. Haugland,et al.  Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates , 1999, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[4]  S. Jun,et al.  Evaluation of a microwire sensor functionalized to detect Escherichia coli bacterial cells. , 2012, Biosensors & bioelectronics.

[5]  Daniele Sblattero,et al.  A deep-blue OLED-based biochip for protein microarray fluorescence detection. , 2013, Biosensors & bioelectronics.

[6]  S. Loeffler.  Artist's Statement , 2011 .

[7]  Marisa Manzano,et al.  Chemiluminescent DNA optical fibre sensor for Brettanomyces bruxellensis detection. , 2012, Journal of biotechnology.

[8]  L. Cocolin,et al.  Bacillus cereus, Bacillus thuringiensis and Bacillus mycoides differentiation using a PCR-RE technique. , 2003, International journal of food microbiology.

[9]  A. Sinai,et al.  The Differential Effect of Toxoplasma Gondii Infection on the Stability of BCL2-Family Members Involves Multiple Activities , 2011, Front. Microbio..

[10]  Ian Papautsky,et al.  Concentration dependence of fluorescence signal in a microfluidic fluorescence detector , 2010 .

[11]  M. Ramuz,et al.  OLED and OPD-based mini-spectrometer integrated on a single-mode planar waveguide chip , 2009 .

[12]  Liduo Wang,et al.  A microfluidic device using a green organic light emitting diode as an integrated excitation source. , 2005, Lab on a chip.

[13]  Vittorio M. N. Passaro,et al.  Guided-Wave Optical Biosensors , 2007, Sensors (Basel, Switzerland).

[14]  J. Oliver.  The viable but nonculturable state in bacteria. , 2005, Journal of microbiology.

[15]  K. Towner,et al.  Evaluation of a real-time PCR hybridization assay for rapid detection of Legionella pneumophila in hospital and environmental water samples. , 2003, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[16]  Xuhua Wang,et al.  Towards microalbuminuria determination on a disposable diagnostic microchip with integrated fluorescence detection based on thin-film organic light emitting diodes. , 2005, Lab on a chip.