论文信息 - Total Synthesis of Acetate from CO2 II. Purification and Properties of Formyltetrahydrofolate Synthetase from Clostridium thermoaceticum

Total Synthesis of Acetate from CO2 II. Purification and Properties of Formyltetrahydrofolate Synthetase from Clostridium thermoaceticum

Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. Km values were: adenosine triphosphate (ATP), 6.25 × 10−4m; formate, 1.67 × 10−3m; and tetrahydrofolate, 1.00 × 10−3m; at pH 8.0. There was a requirement for divalent metals and specifically for ATP.

L. Ljungdahl | H. Wood | A. Y. Sun

[1] E. Stadtman,et al. The conversion of carbon dioxide to acetate. 3. Demonstration of ferredoxin in the system converting Co-14Ch3-cobalamin to acetate. , 1967, Biochemical and biophysical research communications.

[2] E. Stadtman,et al. The conversion of carbon dioxide to acetate. I. The use of cobalt-methylcobalamin as a source of methyl groups for the synthesis of acetate by cell-free extracts of Clostridium thermoaceticum. , 1966, The Journal of biological chemistry.

[3] L. Ljungdahl,et al. Properties of Nicotinamide Adenine Dinucleotide Phosphate-Dependent Formate Dehydrogenase from Clostridium thermoaceticum , 1966, Journal of bacteriology.

[4] E. Stadtman,et al. Synthesis of Co-methyl cobalamin by cell-free extracts of Clostridium thermoaceticum. , 1966, Biochemical and biophysical research communications.

[5] L. Ljungdahl,et al. Total synthesis of acetate from CO2. I. Co-methylcobyric acid and CO-(methyl)-5-methoxybenzimidazolylcobamide as intermediates with Clostridium thermoaceticum. , 1965, Biochemistry.

[6] J. Rabinowitz,et al. Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme. , 1962, The Journal of biological chemistry.

[7] R. Himes,et al. Formyltetrahydrofolate synthetase. II. Characteristics of the enzyme and the enzymic reaction. , 1962, The Journal of biological chemistry.