List of contributors Preface Acknowledgements List of symbols Part I. Chlorophylls and Carotenoids: 1. Microalgal classes and their signature pigments S. W. Jeffrey, S. W. Wright and M. Zapata 2. Recent advances in chlorophyll and bacteriochlorophyll biosynthesis R. J. Porra, U. Oster and H. Scheer 3. Carotenoid metabolism in phytoplankton M. Lohr Part II. Methodology Guidance: 4. New HPLC separation techniques J. L. Garrido, R. L. Airs, F. Rodriguez, L. Van Heukelem and M. Zapata 5. The importance of a quality assurance plan for method validation and minimizing uncertainties in the HPLC analysis of phytoplankton pigments L. Van Heukelem and S. B. Hooker Appendix: a symbology and vocabulary for an HPLC lexicon S. B. Hooker and L. Van Heukelem 6. Quantitative interpretation of chemotaxonomic pigment data H. W. Higgins, S. W. Wright and L. Schluter 7. Liquid chromatography-mass spectrometry for pigment analysis R. L. Airs and J. L. Garrido 8. Multivariate analysis of extracted pigments using spectrophotometric and spectrofluorometric methods J. Neveux, J. Seppala and Y. Dandonneau Appendix: a proven simultaneous equation assay for chlorophylls a and b using aqueous acetone and similar assays for recalcitrant algae R. J. Porra Part III. Water-Soluble 'Pigments': 9. Phycobiliproteins K.-H. Zhao, R. J. Porra and H. Scheer 10. UV-absorbing 'pigments': mycosporine-like amino acids J. I. Carreto, S. Roy, K. Whitehead, C. Llewellyn and M. O. Carignan Part IV. Selected Pigment Applications in Oceanography: 11. Pigments and photoacclimation processes C. Brunet, G. Johnsen, J. Lavaud and S. Roy 12. Pigment-based measurements of phytoplankton rates A. Guttierez-Rodriguez and M. Latasa 13. In vivo bio-optical properties of phytoplankton pigments G. Johnsen, A. Bricaud, N. Nelson, B. B. Prezelin and R. R. Bidigare 14. Optical monitoring of phytoplankton bloom pigment signatures G. Johnsen, M. A. Moline, L. H. Pettersson, J. L. Pinckney, D. V. Pozdnyakov, E. S. Egeland and O. M. Schofield Appendix: harmful algae toxins and pigments E. S. Egeland Part V. Future Perspectives: 15. Perspectives on future directions C. Llewellyn, S. Roy, G. Johnsen, E. S. Egeland, M. Chauton, G. Hallegraeff, M. Lohr, U. Oster, R. J. Porra, H. Scheer and K.-H. Zhao Part VI. Aids for Practical Laboratory Work: Appendix A. Update on filtration, storage and extraction solvents J. L. Pinckney, D. F. Millie and L. Van Heukelem Appendix B. The pigment analyst's guide to HPLC hardware A. R. Neeley, C. S. Thomas, S. B. Hooker and L. Van Heukelem Appendix C. Minimum identification criteria for identifying phytoplankton pigments E. S. Egeland Appendix D. Phytoplankton cultures for standard pigments and their suppliers S. Roy, S. W. Wright and S. W. Jeffrey Appendix E. Commercial suppliers of phytoplankton pigments E. S. Egeland and L. Schluter Part VII. Phytoplankton Pigments Data Sheets E. S. Egeland Index.