Micro-Nanostructured Polyaniline Assembled in Cellulose Matrix via Interfacial Polymerization for Applications in Nerve Regeneration.

Conducting polymers have emerged as frontrunners to be alternatives for nerve regeneration, showing a possibility of the application of polyaniline (PANI) as the nerve guidance conduit. In the present work, the cellulose hydrogel was used as template to in situ synthesize PANI via the limited interfacial polymerization method, leading to one conductive side in the polymer. PANI sub-micrometer dendritic particles with mean diameter of ∼300 nm consisting of the PANI nanofibers and nanoparticles were uniformly assembled into the cellulose matrix. The hydrophobic PANI nanoparticles were immobilized in the hydrophilic cellulose via the phytic acid as "bridge" at presence of water through hydrogen bonding interaction. The PANI/cellulose composite hydrogels exhibited good mechanical properties and biocompatibility as well as excellent guiding capacity for the sciatic nerve regeneration of adult Sprague-Dawley rats without any extra treatment. On the basis of the fact that the pure cellulose hydrogel was an inert material for the neural repair, PANI played an indispensable role on the peripheral nerve regeneration. The hierarchical micro-nanostructure and electrical conductivity of PANI could remarkably induce the adhesion and guiding extension of neurons, showing its great potential in biomedical materials.

[1]  Zhangqi Feng,et al.  Soft Graphene Nanofibers Designed for the Acceleration of Nerve Growth and Development , 2015, Advanced materials.

[2]  Jiang Peng,et al.  Recellularized nerve allografts with differentiated mesenchymal stem cells promote peripheral nerve regeneration , 2012, Neuroscience Letters.

[3]  D. Schade,et al.  Small molecules targeting in vivo tissue regeneration. , 2014, ACS chemical biology.

[4]  M. Dadsetan,et al.  Development of electrically conductive oligo(polyethylene glycol) fumarate-polypyrrole hydrogels for nerve regeneration. , 2010, Biomacromolecules.

[5]  Jie Cai,et al.  A Hierarchical N/S‐Codoped Carbon Anode Fabricated Facilely from Cellulose/Polyaniline Microspheres for High‐Performance Sodium‐Ion Batteries , 2016 .

[6]  A. Nakao,et al.  Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer , 2014, Nature Communications.

[7]  Jerry Silver,et al.  Regeneration beyond the glial scar , 2004, Nature Reviews Neuroscience.

[8]  Lina Zhang,et al.  Highly biocompatible nanofibrous microspheres self-assembled from chitin in NaOH/urea aqueous solution as cell carriers. , 2015, Angewandte Chemie.

[9]  Maurizio Ventre,et al.  Engineering Cell Instructive Materials To Control Cell Fate and Functions through Material Cues and Surface Patterning. , 2016, ACS applied materials & interfaces.

[10]  Yan Liu,et al.  Joint Use of a Chitosan/PLGA Scaffold and MSCs to Bridge an Extra Large Gap in Dog Sciatic Nerve , 2012, Neurorehabilitation and neural repair.

[11]  Jingyan Dong,et al.  Photocured biodegradable polymer substrates of varying stiffness and microgroove dimensions for promoting nerve cell guidance and differentiation. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[12]  Elise M. Stewart,et al.  A Single Component Conducting Polymer Hydrogel as a Scaffold for Tissue Engineering , 2012 .

[13]  J. Mano,et al.  Micro/nano-structured superhydrophobic surfaces in the biomedical field: part II: applications overview. , 2015, Nanomedicine.

[14]  Shanfeng Wang,et al.  Molecularly Engineered Biodegradable Polymer Networks with a Wide Range of Stiffness for Bone and Peripheral Nerve Regeneration , 2015 .

[15]  Lina Zhang,et al.  Highly rate and cycling stable electrode materials constructed from polyaniline/cellulose nanoporous microspheres , 2015 .

[16]  Lina Zhang,et al.  Electromechanical polyaniline–cellulose hydrogels with high compressive strength , 2013 .

[17]  S. Cartmell,et al.  Conductive polymers: towards a smart biomaterial for tissue engineering. , 2014, Acta biomaterialia.

[18]  Zhidan Lin,et al.  New Bacterial Cellulose/Polyaniline Nanocomposite Film with One Conductive Side through Constrained Interfacial Polymerization , 2013 .

[19]  Mohammad Reza Abidian,et al.  Conducting Polymers for Neural Prosthetic and Neural Interface Applications , 2015, Advanced materials.

[20]  Ilsoo Kim,et al.  Enhanced Neurite Outgrowth by Intracellular Stimulation. , 2015, Nano letters.

[21]  Jianhe Liang,et al.  Effect of Octacalcium-Phosphate-Modified Micro/Nanostructured Titania Surfaces on Osteoblast Response. , 2015, ACS applied materials & interfaces.

[22]  T. Zhao,et al.  Bioapplications of hyperbranched polymers. , 2015, Chemical Society reviews.

[23]  Jin-Ye Wang,et al.  Nanostructured Polyaniline Coating on ITO Glass Promotes the Neurite Outgrowth of PC 12 Cells by Electrical Stimulation. , 2015, Langmuir : the ACS journal of surfaces and colloids.

[24]  Jagdish Singh,et al.  Amino acid grafted chitosan for high performance gene delivery: comparison of amino acid hydrophobicity on vector and polyplex characteristics. , 2013, Biomacromolecules.

[25]  Ki-Bum Lee,et al.  Nanotechnology-Based Approaches for Guiding Neural Regeneration. , 2016, Accounts of chemical research.

[26]  B. Fabry,et al.  Biphasic response of cell invasion to matrix stiffness in three-dimensional biopolymer networks. , 2015, Acta biomaterialia.

[27]  Zhongyang Liu,et al.  Electrical regulation of olfactory ensheathing cells using conductive polypyrrole/chitosan polymers. , 2013, Biomaterials.

[28]  David C. Martin,et al.  Effect of Immobilized Nerve Growth Factor on Conductive Polymers: Electrical Properties and Cellular Response , 2007 .

[29]  James B Phillips,et al.  Engineered neural tissue with aligned, differentiated adipose-derived stem cells promotes peripheral nerve regeneration across a critical sized defect in rat sciatic nerve. , 2014, Biomaterials.

[30]  Larry L Hench,et al.  Third-Generation Biomedical Materials , 2002, Science.

[31]  Hedi Mattoussi,et al.  The state of nanoparticle-based nanoscience and biotechnology: progress, promises, and challenges. , 2012, ACS nano.

[32]  M. Abidian,et al.  A Review of Organic and Inorganic Biomaterials for Neural Interfaces , 2014, Advanced materials.

[33]  Lei Tao,et al.  An Injectable, Self‐Healing Hydrogel to Repair the Central Nervous System , 2015, Advanced materials.

[34]  Tal Dvir,et al.  Gold Nanoparticle-Decorated Scaffolds Promote Neuronal Differentiation and Maturation. , 2016, Nano letters.

[35]  Nic D. Leipzig,et al.  Promoting neuron adhesion and growth , 2008 .

[36]  Peter X. Ma,et al.  Conductive PPY/PDLLA conduit for peripheral nerve regeneration. , 2014, Biomaterials.

[37]  Weihua Tang,et al.  Bacterial Cellulose Nanofiber-Supported Polyaniline Nanocomposites with Flake-Shaped Morphology as Supercapacitor Electrodes , 2012 .

[38]  Daryl R. Kipke,et al.  Conducting-polymer nanotubes improve electrical properties, mechanical adhesion, neural attachment, and neurite outgrowth of neural electrodes. , 2010, Small.

[39]  Masami Okamoto,et al.  Synthetic biopolymer nanocomposites for tissue engineering scaffolds , 2013 .

[40]  G. Mali,et al.  Insight into the short-range structure of amorphous iron inositol hexaphosphate as provided by (31)P NMR and Fe X-ray absorption spectroscopy. , 2006, The journal of physical chemistry. B.

[41]  Jun‐Jie Zhu,et al.  Highly Enhanced Fluorescence of CdSeTe Quantum Dots Coated with Polyanilines via In-Situ Polymerization and Cell Imaging Application. , 2015, ACS applied materials & interfaces.

[42]  Lina Zhang,et al.  A bioplastic with high strength constructed from a cellulose hydrogel by changing the aggregated structure , 2013 .

[43]  U. Bora,et al.  In vivo studies of silk based gold nano-composite conduits for functional peripheral nerve regeneration. , 2015, Biomaterials.

[44]  Giada Cellot,et al.  Graphene-Based Interfaces Do Not Alter Target Nerve Cells. , 2016, ACS nano.

[45]  G. Wallace,et al.  Organic conducting polymer-protein interactions , 2012 .

[46]  Jae Young Lee,et al.  Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. , 2009, Biomaterials.

[47]  Xiaojun Yu,et al.  Polycaprolactone and bovine serum albumin based nanofibers for controlled release of nerve growth factor. , 2009, Biomacromolecules.

[48]  A. Harvey,et al.  Hierarchical patterning of multifunctional conducting polymer nanoparticles as a bionic platform for topographic contact guidance. , 2015, ACS nano.

[49]  Lina Zhang,et al.  Dynamic Self-Assembly Induced Rapid Dissolution of Cellulose at Low Temperatures , 2008 .

[50]  Alan P. Koretsky,et al.  TRANSCRANIAL AMELIORATION OF INFLAMMATION AND CELL DEATH FOLLOWING BRAIN INJURY , 2013, Nature.

[51]  Yadong Wang,et al.  Materials for central nervous system regeneration: bioactive cues , 2011 .

[52]  Lina Zhang,et al.  A Facile Construction of Supramolecular Complex from Polyaniline and Cellulose in Aqueous System , 2011 .

[53]  Fabrizio Gelain,et al.  Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections , 2008, BMC biotechnology.

[54]  A. Lloyd,et al.  Macrophage-Induced Blood Vessels Guide Schwann Cell-Mediated Regeneration of Peripheral Nerves , 2015, Cell.

[55]  Lina Zhang,et al.  Effects of Chitin Whiskers on Physical Properties and Osteoblast Culture of Alginate Based Nanocomposite Hydrogels. , 2015, Biomacromolecules.

[56]  Bai-Shuan Liu,et al.  Effects of large-area irradiated laser phototherapy on peripheral nerve regeneration across a large gap in a biomaterial conduit. , 2013, Journal of biomedical materials research. Part A.